اثرات ضد باکتریایی متابولیت خام آسپرژیلوس‌های جدا شده از مرکبات روی استافیلوکوک اورئوس (ATCC 13813)
محورهای موضوعی :
میکروب شناسی
اعظم قربان نیا دلاور
1
,
سید بنیامین مومینی
2
,
سید مسعود هاشمی کروئی
3
,
عیسی غلامپور عزیزی
4
1 - استادیار، گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران
2 - دکتری، دانشکده دامپزشکی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران
3 - استادیار، گروه قارچ‌شناسی، دانشکده دامپزشکی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران
4 - استادیار، گروه قارچ‌شناسی، دانشکده دامپزشکی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران
تاریخ دریافت : 1401/01/22
تاریخ پذیرش : 1401/03/07
تاریخ انتشار : 1401/04/01
کلید واژه:
باکتری,
آسپرژیلوس,
استافیلوکوک اورئوس,
قارچ,
متابولیت قارچی,
مرکبات,
چکیده مقاله :
هدف: کشف گونه‌هایی از میکروارگانیسم‌ها که دارای اثرات ضد میکروبی هستند، دریچه‌ای جدید برای مبارزه با میکروارگانیسم‌ها گشود. قارچ آسپرژیلوس یکی از این میکروارگانیسم‌ها بوده که در راه مبارزه علیه میکروب‌های بیماری‌زا، بسیار اثرگذار است. تمام گونه‌های آسپرژیلوس دارای اثرات ضد میکروبی مناسب و مشابه نیستند و شناسایی و جداسازی گونه‌های دارای اثرات ضد میکروبی، اهمیت دارد.مواد و روش‌ها: در این تحقیق از مرکبات کپک‌زده نمونه‌گیری صورت گرفته و جداسازی و شناسایی آسپرژیلوس‌ها از آنها انجام شد. سه گونه آسپرژیلوس فلاووس، آسپرژیلوس نایجر ایزوله اول و آسپرژیلوس نایجر ایزوله دوم جداسازی شدند. پس از آن متابولیت‌های خام این کپک‌ها در محیط کشت مایع سابرو دکستروز براث، در انکوباتور شیکردار تهیه گردیدند. در نهایت اثر این متابولیت‌ها روی استافیلوکوک اورئوس، از طریق انتشار دیسک با تعیین قطر هاله ممانعت از رشد هر یک از این متابولیت‌ها، روی این باکتری صورت گرفت و پس از آن MICو MBCمحاسبه گردیدند.یافته‌ها: میانگینMIC متابولیت آسپرژیلوس فلاووس روی استافیلوکوک اورئوس 33/208 μl/ml محاسبه شد و میانگین MBC آن روی این باکتری 66/416 μl/ml بدست آمد. میانگین MIC متابولیت آسپرژیلوس نایجر روی استافیلوکوک اورئوس 66/166 μl/ml محاسبه شد و میانگین MBC آن 33/333 μl/ml معین گردید. در اطراف دیسک‌های دارای 150، 165 و 180 میکرولیتر از متابولیت آسپرژیلوس نایجر، میانگین قطر هاله ممانعت از رشد، به ترتیب 8، 8 و 33/8 میلی متر بدست آمدند.نتیجه‌گیری: متابولیت هر آسپرژیلوس اثرات متفاوتی را در جلوگیری از رشد استافیلوکوک اورئوس ایجاد می‌کند.
چکیده انگلیسی:
Objective: Discovering species of microorganisms that have antimicrobial effects opened a new window to fight against microorganisms. Aspergillus fungus is one of these microorganisms that is very effective in fighting against pathogenic microbes. All species of Aspergillus do not have appropriate and similar antimicrobial effects, and it is important to identify and isolate species with antimicrobial effects.Materials and methods: In this research, moldy citrus fruits were sampled and Aspergillus was isolated and identified from them. 3 species of Aspergillus flavus, Aspergillus niger isolate 1 and Aspergillus niger isolate 2 were isolated. After that, the crude metabolites of these molds were prepared in the liquid culture medium of Sabro dextrose broth in Shikardar incubator. Finally, the effect of these metabolites on Staphylococcus aureus was done on this bacterium through disc diffusion by determining the diameter of the growth inhibition halo of each of these metabolites, and then MIC and MBC were calculated.Findings: The average MIC of Aspergillus flavus metabolite on Staphylococcus aureus was calculated to be 208.33 μl/ml and its average MBC on this bacterium was 416.66 μl/ml. The average MIC of Aspergillus niger metabolite on Staphylococcus aureus was calculated to be 166.66 μl/ml and its average MBC was determined to be 333.33 μl/ml. Around the disks with 150, 165 and 180 microliters of Aspergillus niger metabolite, the average diameter of growth inhibition halo was 8, 8 and 8.33 mm, respectively.Conclusion: The metabolite of each Aspergillus produces different effects in preventing the growth of Staphylococcus aureus.
منابع و مأخذ:
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Nacef HS, Belhattab R, Galvez J, Rodriguez SMJ & Vezza T. In Vitro Antimicrobial, Antiviral and Cytotoxicity Activities of Aspergillus oryzae Isolated from El-Baida Marsh in Algeria. Journal of Drug Delivery and Therapeutics. 2020; 10(4): 191-195.
Abdulkawi AA & Abdulgabbar A. Over view on antibacterial metabolites from terrestrial Aspergillus spp. Mycology. 2019; 10(4): 191-209.
Dhurgham AH, Alhasan Husein A & Husein Ahmed Q Dawood. In Vitro Antimicrobial Activity of The Filtrate Crude Extract Produced by Aspergillus niger. University Thi-Qar Journal Sciences. 2019; 7(1): 66-71.
Lijian D, Lu R, Shuang L, Jingjing S, Zhiwen H, Shan H & Shihai X. Production of New Antibacterial 4 Hydroxy-α-Pyrones by a Marine Fungus Aspergillus niger Cultivated in Solid Medium. Mar Drugs. 2019; 17(344): 1-8.
Borjian Brojeni S, Kaveh Babaheydari E, Mortezaei S, Karimian M, Shirzad M & Validi M. The Antibacterial Effects of the Hydroalcoholic Extracts of Aloe Vera and Glycyrrhiza Glabra against Cariogenic Bacteria InVitro. Journal Babol University Medical Sciences. 2016; 18(4): 14-20. [in persian]
Ghada A, Fawzy Areej M, Al-Taweel & Nahla A M. In Vitro Antimicrobial and Anti-Tumor Activities of Intracellular and Extracellular extracts of Aspergillus niger and Aspergillus flavus var, columinari .Journal of Pharmaceutical Sciences and Research. 2011; 3(1): 980-987.
Safarkar R, Bonabi R, Massiha A, Rezaei Nazifi M & Sotoudeh R. An Evaluation of the Inhibitory and Synergistic Effects of Alcoholic Extract of Stachys Byzantina on Standard Strains under in vitro Conditions. Journal Babol University Medical Science 2017; 19(5): 39-46. [in persian]
Azam Taheri A, GholampourAzizi I, Hashemi M, Farhadi L, Servatyari K & Rouhi S. Inhibitory effect of aquatic and alcoholic extracts of Artemisia sieberi on growth of Candida Albicans: An in vitro study. Qom University Medical Sciences Journal. 2018; 12(6): 39-47. [in persian]
Gholampour Azizi I, Rouhi S, Zandi S, Kashefi H & Hassanzadeh Miandasteh S. Antifungal effect of melia azedarach alcoholic and aquatic extract on Malassezia furfur. Novin Health 2017; 1(2): 7-11. [in persian]
Najafi M, Gholampour Azizi I, Hashemi Karouei M, Khani D & Rouhi S. Investigation of the anti-growth effect of hydroalcoholic extract of Artemisia deserti on Malassezia furfur isolated from clinical specimen. Razi Journal Medical Sciences. 2018; 25(166): 100-106. [in persian]
Sajadi Kaboodi P, Bakhshi D & Moghadamnia AA, Sefidgar A. The Antibacterial Effects of Methanol Extract of Ammi majus on Staphylococcus aureus and Escherichia coli. Journal Babol University Medcal Science 2017; 19(1): 36-42. [in persian]
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Kavanagh K. Fungal fermentations systems and products. In: Kavanagh K (ed.). Fungi: biology and applica- UK: Wiley-Blackwell. 2011:125-146.
Kalyani P & Hemalatha K. In vitro antimicrobial potential of Aspergillus niger. (MTCC-961). International Journal of Chemistry Technical Research. 2017; 10(4): 430-435.
Yahaya S, Idris B, Yusuf I & Rabiu MK. Screening and identification of antibacterial agents produced by Aspergillus species from the soil of Bayero University Kano. Bayero Journal Pure Applied Sciences. 2017; 10(1): 498–502.
Rani R, Sharma D, Chaturvedi M & Yadav J P. Antibacterial activity of twenty different endophytic fungi isolated from Calotropis procera and time kill assay. Clinical Microbiology. 2017; 6(3): 1-6.
Abdulwahid BA, Al-Shaibani Faiz I, Al-Shakarchi & Rasha SA. Extraction and Characterization of Antibacterial Compound from Aspergillus niger. Journal Al-Nahrain University. 2013; 16(4): 167-174.
_||_
Jaberi Ansari F, Jafari Mansoorian H, Jalili H & Azizi M. A Review of the Effective Factors for Lovastatin Production by Aspergillus Terreus Atcc 20542 in Liquid Submerged Fermentation. Journal Babol University Medical Science 2016; 18(12):
40-8. [in persian]
El-hawary S S, Moawad A S, Bahr H S, Abdelmohsen U R & Mohammed R. Natural product diversity from the endophytic fungi of the genus Aspergillus. RSC Advances. 2020; 10(37): 22058-22079.
Gholampour Azizi I. Mycotoxins. Sadaf. Shekofeh yas. 2011: 23-28. [in persian]
Karuppusamy S. A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plants Research. 2009; 3(13): 1222-1239.
Sumner L, Lei W, Nikolau Z & Saito K. Modern plant metabolomics: advanced natural product gene discoveries, improved technologies, and future prospects. Natural product reports. 2015; 32(2): 212-229.
Goyal S, Ramawat K G & Mérillon J M. Different shades of fungal metabolites an overview. Fungal metabolites. 2016; 1-29. DOI:1007/978-3-319-19456-1_34-1
Nacef HS, Belhattab R, Galvez J, Rodriguez SMJ & Vezza T. In Vitro Antimicrobial, Antiviral and Cytotoxicity Activities of Aspergillus oryzae Isolated from El-Baida Marsh in Algeria. Journal of Drug Delivery and Therapeutics. 2020; 10(4): 191-195.
Abdulkawi AA & Abdulgabbar A. Over view on antibacterial metabolites from terrestrial Aspergillus spp. Mycology. 2019; 10(4): 191-209.
Dhurgham AH, Alhasan Husein A & Husein Ahmed Q Dawood. In Vitro Antimicrobial Activity of The Filtrate Crude Extract Produced by Aspergillus niger. University Thi-Qar Journal Sciences. 2019; 7(1): 66-71.
Lijian D, Lu R, Shuang L, Jingjing S, Zhiwen H, Shan H & Shihai X. Production of New Antibacterial 4 Hydroxy-α-Pyrones by a Marine Fungus Aspergillus niger Cultivated in Solid Medium. Mar Drugs. 2019; 17(344): 1-8.
Borjian Brojeni S, Kaveh Babaheydari E, Mortezaei S, Karimian M, Shirzad M & Validi M. The Antibacterial Effects of the Hydroalcoholic Extracts of Aloe Vera and Glycyrrhiza Glabra against Cariogenic Bacteria InVitro. Journal Babol University Medical Sciences. 2016; 18(4): 14-20. [in persian]
Ghada A, Fawzy Areej M, Al-Taweel & Nahla A M. In Vitro Antimicrobial and Anti-Tumor Activities of Intracellular and Extracellular extracts of Aspergillus niger and Aspergillus flavus var, columinari .Journal of Pharmaceutical Sciences and Research. 2011; 3(1): 980-987.
Safarkar R, Bonabi R, Massiha A, Rezaei Nazifi M & Sotoudeh R. An Evaluation of the Inhibitory and Synergistic Effects of Alcoholic Extract of Stachys Byzantina on Standard Strains under in vitro Conditions. Journal Babol University Medical Science 2017; 19(5): 39-46. [in persian]
Azam Taheri A, GholampourAzizi I, Hashemi M, Farhadi L, Servatyari K & Rouhi S. Inhibitory effect of aquatic and alcoholic extracts of Artemisia sieberi on growth of Candida Albicans: An in vitro study. Qom University Medical Sciences Journal. 2018; 12(6): 39-47. [in persian]
Gholampour Azizi I, Rouhi S, Zandi S, Kashefi H & Hassanzadeh Miandasteh S. Antifungal effect of melia azedarach alcoholic and aquatic extract on Malassezia furfur. Novin Health 2017; 1(2): 7-11. [in persian]
Najafi M, Gholampour Azizi I, Hashemi Karouei M, Khani D & Rouhi S. Investigation of the anti-growth effect of hydroalcoholic extract of Artemisia deserti on Malassezia furfur isolated from clinical specimen. Razi Journal Medical Sciences. 2018; 25(166): 100-106. [in persian]
Sajadi Kaboodi P, Bakhshi D & Moghadamnia AA, Sefidgar A. The Antibacterial Effects of Methanol Extract of Ammi majus on Staphylococcus aureus and Escherichia coli. Journal Babol University Medcal Science 2017; 19(1): 36-42. [in persian]
Espinel-Ingroff AV & Pfaller MA. Susceptibility test methods: yeasts and filamentous in fungi. In: Manual of clinical microbiology, 9th Washington, DC: ASM Press: 2007.
Kavanagh K. Fungal fermentations systems and products. In: Kavanagh K (ed.). Fungi: biology and applica- UK: Wiley-Blackwell. 2011:125-146.
Kalyani P & Hemalatha K. In vitro antimicrobial potential of Aspergillus niger. (MTCC-961). International Journal of Chemistry Technical Research. 2017; 10(4): 430-435.
Yahaya S, Idris B, Yusuf I & Rabiu MK. Screening and identification of antibacterial agents produced by Aspergillus species from the soil of Bayero University Kano. Bayero Journal Pure Applied Sciences. 2017; 10(1): 498–502.
Rani R, Sharma D, Chaturvedi M & Yadav J P. Antibacterial activity of twenty different endophytic fungi isolated from Calotropis procera and time kill assay. Clinical Microbiology. 2017; 6(3): 1-6.
Abdulwahid BA, Al-Shaibani Faiz I, Al-Shakarchi & Rasha SA. Extraction and Characterization of Antibacterial Compound from Aspergillus niger. Journal Al-Nahrain University. 2013; 16(4): 167-174.
