Small Intestine Microvilli Status, Immune System and Broilers Chickens Performance in Response to Threonine Requirement
محورهای موضوعی :M. Agha Basiri Sarabi 1 , A.A. Saki 2 , H. Jahanian Najafabadi 3 , A. Ahmadi 4 , Z. Bardel 5
1 - Department of Animal Science, Faculty of Agricultrue, Bu-Ali Sina University, Hamedan, Iran
2 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
3 - Department of Animal Science, Faculty of Agricultrue, Bu-Ali Sina University, Hamedan, Iran
4 - Department of Animal Science, Faculty of Agricultrue, Bu-Ali Sina University, Hamedan, Iran
5 - Department of Animal Science, Faculty of Agricultrue, Bu-Ali Sina University, Hamedan, Iran
کلید واژه: broiler chickens, microvilli, requirement, threonine,
چکیده مقاله :
This study was conducted to determine different levels of threonine on intestinal microvilli morphology, humoral immunity, carcass content and performance in broiler chickens. A total of 272 day-old broiler chickens (Ross 308) with 4 treatments, 4 replicates and 17 birds in each were arranged in completely ran-dom design (CRD). Treatments were included 4 levels of starter, grower and finisher threonine in diets (0.96, 0.91, 0.86, 0.81); (0.87, 0.82, 0.77, 0.72); (0.78, 0.73, 0.68, 0.63%) respectively. At the end of the experiment, intestinal morphology, performance and blood parameters were measured. The results showed that the primary immune response against red blood cells was reduced (P<0.05) by decreased dietary threonine levels, in contrast, the amount of IgG and total antibody were increased with increasing levels of dietary threonine, (P<0.05). Increased threonine levels at 42 days of age resulted in higher lymphocyte counts and an elevated heterophil-to-lymphocyte ratio (P<0.05). Increased villus length, crypt depth, and villus length to crypt depth ratio were indicated by the high levels of threonine in the diet (P<0.05). The numbers and height of microvilli were reduced by decreasing threonine levels. In general, it can be con-cluded that increasing the level of threonine in the diet improved intestinal condition, which in turn could improve performance.
This study was conducted to determine different levels of threonine on intestinal microvilli morphology, humoral immunity, carcass content and performance in broiler chickens. A total of 272 day-old broiler chickens (Ross 308) with 4 treatments, 4 replicates and 17 birds in each were arranged in completely ran-dom design (CRD). Treatments were included 4 levels of starter, grower and finisher threonine in diets (0.96, 0.91, 0.86, 0.81); (0.87, 0.82, 0.77, 0.72); (0.78, 0.73, 0.68, 0.63%) respectively. At the end of the experiment, intestinal morphology, performance and blood parameters were measured. The results showed that the primary immune response against red blood cells was reduced (P<0.05) by decreased dietary threonine levels, in contrast, the amount of IgG and total antibody were increased with increasing levels of dietary threonine, (P<0.05). Increased threonine levels at 42 days of age resulted in higher lymphocyte counts and an elevated heterophil-to-lymphocyte ratio (P<0.05). Increased villus length, crypt depth, and villus length to crypt depth ratio were indicated by the high levels of threonine in the diet (P<0.05). The numbers and height of microvilli were reduced by decreasing threonine levels. In general, it can be con-cluded that increasing the level of threonine in the diet improved intestinal condition, which in turn could improve performance.
Abdelqader A. and Al-Fataftah A.R. (2016). Effect of dietary butyricacid on performance, intestinal morphology, microflora composition and intestinal recovery of heat-stressed broilers. Livest. Sci. 183, 78-83.
Ahmed I., Qaisrani S.N., Azam F., Pasha T.N., Bibi F., Naveed S. and Murtaza S. (2020). Interactive effects of threonine levels and protein source on growth performance and carcass traits, gut morphology, ileal digestibility of protein and amino acids, and immunity in broilers. Poult. Sci. 99(1), 280-289.
Azzam M.M.M. and El-Gogary M.R. (2015). Effects of dietary threonine levels and stocking density on the performance, metabolic status and immunity of broiler chickens. Asian J. Anim. Vet. Adv. 10(5), 215-225.
Ball R.O. (2001). Threonine requirement and the interaction be-tween threonine intake and gut mucins in pigs. Pp. 58-68 in Proc. 2001 Degussa Tech. Symp., Banff Pork Seminar, Banff, Alberta, Canada.
Bassareh M., Rezaeipour V., Abdullahpour R. and Asadzadeh S. (2023). Dietary threonine and lysophospholipid supplement in broiler chickens: effect on productive performance, carcass variables, cecal microbiota activity, and jejunal morphol-ogy. Trop. Anim. Health Prod. 55(3), 150-161.
Berres J., Vieira S.L., Coneglian J.L.B., Olmos A.R., Md D., Bor-tolini Freitas T.C.K. and da Silva G.X. (2007). Broiler re-sponses to graded increases in the threonine to lysine ratio. Ciênc. Rural. 37(2), 510-517.
Cabellos J., Delpivo C., Vázquez-Campos S. and Janer G. (2019). In vitro assessment of CeO2 nanoparticles effects on intestinal microvilli morphology. Toxicol. in Vitro. 59, 70-77.
Chee S.H., Iji P.A., Choct M., Mikkelsen L.L. and Kocher A. (2010). Functional interactions of manno-oligosaccharides with dietary threonine in chicken gastrointestinal tract. I. Growth performance and mucin dynamics. Br. Poult. Sci. 51(5), 658-666.
Chen Y.P., Cheng Y.F., Li X.H., Yang W.L., Wen C., Zhuang S. and Zhou Y.M. (2017). Effects of threonine supplementation on the growth performance, immunity, oxidative status, intes-tinal integrity, and barrier function of broilers at the early age. Poult. Sci. 96(2), 405-413.
Corzo A., Kidd M.T., Dozier III W.A., Pharr G.T. and Koutsos E.A. (2007). Dietary threonine needs for growth and immunity of broilers raised under different litter conditions. J. Appl. Poult. Res. 16(4), 574-582.
De Barros Moreira Filho A.L., de Oliveira C.J.B., de Oliveira H.B., Campos D.B., Guerra R.R., Costa F.G.P. and Givisiez P.E.N. (2015). High incubation temperature and threonine die-tary level improve ileum response against post-hatch salmo-nella enteritidis inoculation in broiler chicks. PLoS One. 10(7), e0131474.
Debnath B.C., Biswas P. and Roy B. (2019). The effects of sup-plemental threonine on performance, carcass characteristics, immune response and gut health of broilers in subtropics dur-ing pre-starter and starter period. J. Anim. Physiol. Anim. Nutr. 103(1), 29-40.
Debnath B.C., Biswas P., Roy B. and Chatterjee P.N. (2020). Threonine supplementation on gut histomorphometry and mucin biology of broilers. Indian J. Anim. Health. 59(2), 206-209.
Dozier W.A., Moran E.T. and Kidd M.T. (2000). Threonine re-quirement for broiler males from 42 to 56 days of age. J. Appl. Poult. Res. 9(2), 214-222.
Eftekhari A., Rezaeipour V. and Abdullahpour R. (2015). Effects of acidified drinking water on performance, carcass, immune response, jejunum morphology, and microbiota activity of broiler chickens fed diets containing graded levels of threonine. Livest. Sci. 180, 158-163.
Estalkhzir F.M., Khojasteh S. and Jafari M. (2013). The effect of different levels of threonine on performance and carcass char-acteristics of broiler chickens. J. Novel Appl. Sci. 2(9), 382-386.
Faure M., Moënnoz D., Montigon F., Mettraux C., Breuillé D. and Ballevre O. (2005). Dietary threonine restriction specifically reduces intestinal mucin synthesis in rats. J. Nutr. 135(3), 486-491.
Faust J.J., Doudrick K., Yang Y., Westerhoff P. and Capco D.G. (2014). Food grade titanium dioxide disrupts intestinal brush border microvilli in vitro independent of sedimentation. Cell Biol. Toxicol. 30(3), 169-188.
Ghanima M.M.A., Abd El-Hack M.E., Al-Otaibi A.M., Nasr S., Almohmadi N.H., Taha A.E., Jaremko M. and El-Kasrawy N.I. (2023). Growth performance, liver and kidney functions, blood hormonal profile, and economic efficiency of broilers fed different levels of threonine supplementation during feed restriction. Poult. Sci. 102(8), 102796-102802.
Ghasemi H.A. (2022). Effects of dietary electrolyte balance and digestible threonine on intestinal morphology, microbial oopu-lation and digestibility of broilers subjected to heat stress con-ditions. J. Vet. Res. 77(2), 63-78.
Han Y. and Baker D.H. (1991). Lysine requirements of fast-and slow-growing broiler chicks. Poult. Sci. 70(10), 2108-2114.
Horn N.L., Donkin S.S., Applegate T.J. and Adeola O. (2009). Intestinal mucin dynamics: response of broiler chicks and White Pekin ducklings to dietary threonine. Poult. Sci. 88(9), 1906-1914.
Jahanian R. (2010). Threonine needs of growing broiler chickens for performance and optimum immunological functions in re-sponse to dietary crude protein concentration. Pp. 41-48 in Proc. 2nd Int. Vet. Poult. Congr., Tehran, Iran.
Khan A.R., Nawaz H. and Zahoor I. (2006). Effect of different levels of digestible threonine on growth performance of broiler chicks. J. Anim. Plant Sci. 16(1), 8-11.
Kidd M.T. and Kerr B.J. (1997). Threonine responses in commer-cial broilers at 30 to 42 days. J. Appl. Poult. Res. 6(4), 362-367.
Law J., Adjiri-Awere A., Pencharz P.B. and Ball R.O. (2000). Gut mucins in piglets are dependent upon dietary threonine. Pp. 51-59 in Proc. Adv. Pork Prod., Alberta, Canada.
Lemme A., Ravindran V. and Bryden W.L. (2004). Ileal digesti-bility of amino acids in feed ingredients for broilers. World's Poult. Sci. J. 60(4), 423-438.
Mack S., Bercovici D., DeGroote G., Leclercq B., Lippens M., Pack M., Schutte J.B. and Van Cauwenberghe S. (1999). Ideal amino acid profile and dietary lysine specification for broiler chickens of 20 to 40 days of age. Br. Poult. Sci. 40(2), 257-265.
Min Y.N., Liu S.G., Qu Z.X., Meng G.H. and Gao Y.P. (2017). Effects of dietary threonine levels on growth performance, se-rum biochemical indexes, antioxidant capacities, and gut mor-phology in broiler chickens. Poult. Sci. 96(5), 1290-1297.
Nakajima T., Kishl H., Kusubae T., Wakamalsu H. and Kusulani Y. (1985). Effect of L-threonine and DL-tryptophan supple-mentation to the low protein practical broiler finisher diet. Japanese Poult. Sci. 22(1), 10-16.
Nichols N.L. and Bertolo R.F. (2008). Luminal threonine concen-tration acutely affects intestinal mucosal protein and mucin synthesis in piglets. J. Nutr. 138(7), 1298-1303.
Pinchuk G. (2002). Schaum's Outline of Theory and Problems of Immunology. McGraw-Hill, New York.
Qaisrani S.N., Ahmed I., Azam F., Bibi F., Pasha T.N. and Azam F. (2018). Threonine in broiler diets: an updated review. Ann. Anim. Sci. 18(3), 659-674.
Qaisrani S.N., Van Krimpen M.M., Kwakkel R.P., Verstegen M.W.A. and Hendriks W.H. (2015). Dietary factors affecting hindgut protein fermentation in broilers: A review. World’s Poult. Sci. J. 71(1), 139-160.
Rama Rao S.V., Raju M.V., Panda L.N., Poonam A.K., Moorthy N.S., Srilatha O.K. and Shyam Sunder T.R. (2011). Perform-ance, carcass variables and immune responses in commercial broiler chicks fed graded concentrations of threonine in diet containing sub-optimal levels of protein. Poult. Sci. 169(3), 218-223.
Rezaeipour V., Fononi H. and Irani M. (2012). Effects of dietary L-threonine and Saccharomyces cerevisiae on performance, intestinal morphology and immune response of broiler chick-ens. South African J. Anim. Sci. 42(3), 266-273.
SAS Institute. (2017). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Shan A.S., Sterling K.G., Pesti G.M., Bakalli R.I., Driver J.P. and Atencio T. (2002). The influence of temperature on the threonine requirement of young broiler chicks. Pp. 51-59 in 91st Ann. Meet Poult. Sci. Assoc., Paris, France.
Shirzadegan K., Nickkhah I. and Jafari M.A. (2015). Impacts of dietary threonine supplementation on performance and intesti-nal morphology of broiler chickens during summer time. Iran. J. Appl. Anim. Sci. 5(2), 431-436.
Sigolo S., Zohrabi Z., Gallo A., Seidavi A. and Prandini A. (2017). Effect of a low crude protein diet supplemented with different levels of threonine on growth performance, carcass traits, blood parameters, and immune responses of growing broilers. Poult. Sci. 96(8), 2751-2760.
Smirnov A., Tako E., Ferket P.R. and Uni Z. (2006). Mucin gene expression and mucin content in the chicken intestinal goblet cells are affected by in ovo feeding of carbohydrates. Poult. Sci. 85(4), 669-673.
Soltan M.A. (2009). Influence of dietary glutamine supplementa-tion on growth performance, small intestinal morphology, immune response and some blood parameters of broiler chick-ens. Int. J. Poult. Sci. 8(1), 60-68.
Stedman N.L., Brown T.P., Brooks Jr R.L. and Bounous D.I. (2001). Heterophil function and resistance to staphylococcal challenge in broiler chickens naturally infected with avian leu-kosis virus subgroup. J. Vet. Pathol. 38(5), 519-527.
Stoll B. (2006). Intestinal uptake and metabolism of threonine: Nutritional impact. Adv. Pork Prod. 17, 257-263.
Zaghari M., Zaefarian F. and Shivazad M. (2012). Standardized ileal digestible threonine requirements and its effects on per-formance and gut morphology of broiler chicks fed two levels of protein. J. Agric. Sci. Technol. 13(4), 541-552.
Zhang Q., Chen X., Eicher S.D., Ajuwon K.M. and Applegate T.J. (2016). Effect of threonine deficiency on intestinal integrity and immune response to feed withdrawal combined with coc-cidial vaccine challenge in broiler chicks. Br. J. Nutr. 116(12), 2030-2043.
