Cytotoxic effects of hydroalcoholic extract of Iranian cranberry (Vaccinium arctostaphylos) on breast cancer cell lines MCF-7 and MDA-MB2321
محورهای موضوعی : مجله گیاهان داروییArmin Deyhim 1 , Ali Sharifzadeh 2
1 - Department of Veterinary Medicine, Shk.C., Islamic Azad University, Shahrekord, Iran;
2 - Department of Veterinary Microbiology, Shk.C., Islamic Azad University, Shahrekord, Iran;
کلید واژه: Breast cancer, Cranberry, MCF-7, MDA-MB231,
چکیده مقاله :
. Background & Aim: Cancer is currently recognized as the second leading cause of death worldwide, following cardiovascular diseases, with breast cancer being the most common malignant tumor among women. Despite ongoing efforts, there is still no successful treatment for this disease, and chemotherapy, while widely used, not only fails to guarantee a cure but also carries significant side effects. This study aimed to evaluate the cytotoxic effects of Iranian cranberry (Vaccinium arctostaphylos) extract on human breast cancer cell lines. Experimental: The MCF-7 and MDA-MB231 cell lines were obtained from the Geniran Institute, and after cell culture and extract preparation, concentrations of 25 µg/mL, 50 µg/mL, 75 µg/mL, 100 µg/mL, 125 µg/mL, 150 µg/mL, 175 µg/mL, and 200 µg/mL were tested on 96-well plates for 24, 48, and 72 h. Cell viability was assessed using the MTT assay. Results: The results indicated that the concentration of 200 µg/mL after 72 h had the greatest impact on reducing the survival of cancer cells, eliminating over 70% of cancer cells in both lines during this period. Given the significant efficacy of the Iranian cranberry extract in eliminating cancer cells in vitro, further clinical studies on this plant are recommended. Recommended applications/industries: According to the results obtained, the extract of Iranian cranberry has the potential to be used in pharmaceutical industries and research centers as a supplement for the treatment of breast cancer. Of course, further clinical studies on this extract are recommended.
. Background & Aim: Cancer is currently recognized as the second leading cause of death worldwide, following cardiovascular diseases, with breast cancer being the most common malignant tumor among women. Despite ongoing efforts, there is still no successful treatment for this disease, and chemotherapy, while widely used, not only fails to guarantee a cure but also carries significant side effects. This study aimed to evaluate the cytotoxic effects of Iranian cranberry (Vaccinium arctostaphylos) extract on human breast cancer cell lines. Experimental: The MCF-7 and MDA-MB231 cell lines were obtained from the Geniran Institute, and after cell culture and extract preparation, concentrations of 25 µg/mL, 50 µg/mL, 75 µg/mL, 100 µg/mL, 125 µg/mL, 150 µg/mL, 175 µg/mL, and 200 µg/mL were tested on 96-well plates for 24, 48, and 72 h. Cell viability was assessed using the MTT assay. Results: The results indicated that the concentration of 200 µg/mL after 72 h had the greatest impact on reducing the survival of cancer cells, eliminating over 70% of cancer cells in both lines during this period. Given the significant efficacy of the Iranian cranberry extract in eliminating cancer cells in vitro, further clinical studies on this plant are recommended. Recommended applications/industries: According to the results obtained, the extract of Iranian cranberry has the potential to be used in pharmaceutical industries and research centers as a supplement for the treatment of breast cancer. Of course, further clinical studies on this extract are recommended.
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