Cytotoxic effects of hydroalcoholic extract of Iranian cranberry (Vaccinium arctostaphylos) on breast cancer cell lines MCF-7 and MDA-MB2321
الموضوعات : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)Armin Deyhim 1 , Ali Sharifzadeh 2
1 - Department of Veterinary Medicine, Shk.C., Islamic Azad University, Shahrekord, Iran;
2 - Department of Veterinary Microbiology, Shk.C., Islamic Azad University, Shahrekord, Iran;
الکلمات المفتاحية: Breast cancer, Cranberry, MCF-7, MDA-MB231,
ملخص المقالة :
. Background & Aim: Cancer is currently recognized as the second leading cause of death worldwide, following cardiovascular diseases, with breast cancer being the most common malignant tumor among women. Despite ongoing efforts, there is still no successful treatment for this disease, and chemotherapy, while widely used, not only fails to guarantee a cure but also carries significant side effects. This study aimed to evaluate the cytotoxic effects of Iranian cranberry (Vaccinium arctostaphylos) extract on human breast cancer cell lines. Experimental: The MCF-7 and MDA-MB231 cell lines were obtained from the Geniran Institute, and after cell culture and extract preparation, concentrations of 25 µg/mL, 50 µg/mL, 75 µg/mL, 100 µg/mL, 125 µg/mL, 150 µg/mL, 175 µg/mL, and 200 µg/mL were tested on 96-well plates for 24, 48, and 72 h. Cell viability was assessed using the MTT assay. Results: The results indicated that the concentration of 200 µg/mL after 72 h had the greatest impact on reducing the survival of cancer cells, eliminating over 70% of cancer cells in both lines during this period. Given the significant efficacy of the Iranian cranberry extract in eliminating cancer cells in vitro, further clinical studies on this plant are recommended. Recommended applications/industries: According to the results obtained, the extract of Iranian cranberry has the potential to be used in pharmaceutical industries and research centers as a supplement for the treatment of breast cancer. Of course, further clinical studies on this extract are recommended.
Alimohammadi, M., Rahimi, A., Faramarzi, F., Golpour, M., Jafari-Shakib, R., Alizadeh-Navaei R. 2021. Effects of coenzyme Q10 supplementation on inflammation, angiogenesis, and oxidative stress in breast cancer patients: a systematic review and meta-analysis of randomized controlled-trials. Inflammopharmacology, 29(3): 579-93.
Ayaz, F.A., Hayirlioglu-Ayaz, S., Gruz, J., Novak, O. and Strand, M. 2005.Separation, characterization, and quantitation of phenolic acids in a little-known blueberry (Vaccinium arctostaphylos L.) fruit by HPLC-MS. Journal of Agriculture and Food Chemistry, 53(21):8116-22.
Bellanger, M., Zeinomar, N., Tehranifar, P. and Terry, M.B. 2018. Are global breast cancer incidence and mortality patterns related to country-specific economic development and prevention strategies? JCO Global Oncology, 4: 1–16.
Boivin, D., Blanchette, M., Barrette, S., Moghrabi, A. and Béliveau, R. 2007. Inhibition of cancer cell proliferation and suppression of TNF-induced activation of NFkappaB by edible berry juice. Anticancer Research, 27(2): 937-948.
Burstein, H.J., Curigliano, G., Loibl, S., Dubsky, P., Gnant, M., Poortmans, P., Colleoni, M., Denkert, C., Piccart-Gebhart, M. and Regan, M. 2019. Estimating the benefits of therapy for early-stage breast cancer: The St. Gallen International Consensus Guidelines for the primary therapy of early breast cancer 2019. Annals Oncology, 30: 1541–1557.
Ghoncheh, M., Pournamdar, Z. and Salehiniya, H. 2016. Incidence and mortality and epidemiology of breast cancer in the world. Asian Pacific Journal of Cancer Prevention, 17: 43–46.
Gorbanzadeh, E and Zaefizadeh, M. 2018. Inhibitory effects of Vaccinium arctostaphylos extract on prostate cancer cells. Journal of Ardabil University of Medical Sciences,17(4): 487-496.
Hasanloo, T., JafarkhaniKermani, M., Dalvand, Y.A. and Rezazadeh Sh. 2013. A complete review on the genus Vaccinium and Iranian Ghareghat. Quarterly Journal of Medicinal Plants, 18(72): 46-65.
Karami, F., Shahnazari, M., Ebrahimi, A., Khosrowchahli, M. 2022. Investigating antitumorigenic effects of Vaccinium Arctostaphylos on colorectal cancer cells. Food & Health, 5(2):15-19.
Lee, K., Kruper, L., Dieli-Conwright, C.M. and Mortimer, J.E. 2019. The impact of obesity on breast cancer diagnosis and treatment. Current Oncology Report, 21 (5): 41.
Liao, G.S., Apaya, M.K., Shyur, L.F. 2013. Herbal medicine and acupuncture for breast cancer palliative care and adjuvant therapy. Evidence‐Based Complementary and Alternative Medicine. 2013: 437948.
Lima, S.M., Kehm, R.D. and Terry, M.B. 2021. Global breast cancer incidence and mortality trends by region, age-groups, and fertility patterns. eClinical Medicine, 38: 100-985.
Mehrgou, A. and Akouchekian, M. 2016. The importance of BRCA1 and BRCA2 genes mutations in breast cancer development. Medical Journal of the Islamic Republic of Iran, 30: 369-375.
Nardin, S., Mora, E., Varughese, F.M., D’Avanzo, F., Vachanaram, A.R., Rossi, V., Saggia, C., Rubinelli, S. and Gennari, A. 2020. Breast cancer survivorship, quality of life, and late toxicities. Frontiers in Oncology, 16: 864.
Prasad, S., Ravindran, J. and Aggarwal, B.B. 2010. NF-kappaB and cancer: How intimate is this relationship. Molecular and Cellular Biochemistry, 336: 25–37.
Religioni, U. 2020.Cancer incidence and mortality in Poland. Clinical Epidemiology and Global Health, 8: 329–334.
Sabale, P., Modi, A. and Sabale, V.2013. Curcuma longa Linn. A phytochemical and phytopharmacological review. Research Journal of Pharmacognosy and Phytochemistry, 5(2): 59-65.
Samulak D., Pięta B., Michalska M., Grzelak W., Sajdak S. 2008. Oszczędzające metody diagnostyczno-terapeutyczne guzów gruczołu sutkowego. Ginekol Prakt, 4:6–9.
Scartezzini, P., Speroni, E. 2000. Review on some plants of Indian traditional medicine with antioxidant activity. Journal of Ethnopharmacology, 71: 1-2
Sepehrifar, R. and Hasanloo, T. 2010. Polyphenolic, flavonoids and anthoccyanins content and antioxidant activity of Vaccinium arctostaphylos L. from different areas of Iran. Journal of Medicinal Plants, 9(33): 66-74.
Sharifzadeh, A., Aali, F. and Vahhabi, S. 2021. The survey of anticancer and anti-microbial properties of Rosmarinus officinalis L, Peganum harmala L and coffee extracts on cell viability of cancer cells. Journal of Food Microbiology, 8(1): 14-24.
Siddiqui, B., Anthony, V., Pathak, K., Singh, S., Chandrakar, R. and Jain, D. 2022. Review on herbal drugs nanonization for the treatment of breast cancer. Biological Sciences, 2(3): 291-301.
Smolarz, B., Nowak, A.Z. and Romanowicz, H.2022. Breast cancer—epidemiology, classification, pathogenesis and treatment (Review of literature). Cancers, 14: 2569.
Sun, J. and Liu, R.H. 2006. Cranberry phytochemical extracts induce cell cycle arrest and apoptosis in human MCF-7 breast cancer cells, Cancer Letters. 241(10): 124-134.
Tornatore, L., Thotakura, A.K., Bennett, J., Moretti, M. and Franzoso, G. 2012. The nuclear factor kappa B signaling pathway: Integrating metabolism with inflammation. Trends in Cell Biology, 22: 557–566.
Torre, L.A., Islami, F., Siegel, R.L., Ward, E.M. and Jemal, A. 2017. Global cancer in women: burden and trends. Cancer Epidemiology, Biomarkers and Prevention, 26: 444–457.
Towpik E. 2018. In: Rola Chirurgii w Rozpoznawaniu i Leczeniu Raka Piersi. Pawlicki M., Ed. Alfa-Medica Press; Bielsko-Biała, Poland. Rak Piersi, Nowe Nadzieje i Możliwości Leczenia. pp. 30–37.
Towpik, E., RolaChirurgii w Rozpoznawaniui LeczeniuRaka Piersi; Pawlicki, M., Ed. 2018. RakPiersi, NoweNadziejeiMo˙zliwo´sciLeczenia; Alfa-Medica Press: Bielsko-Biała, Poland, 30–37.
Tyagi, A., Sharma, S., Wu, K., Wu, S.Y., Xing, F., Liu, Y., Zhao, D., Deshpande, R.P., D’Agostino, R.B. and Jr. Watabe, K. 2021. Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre-metastatic niche in lung. Nature Communication, 12: 474.
Wani, K. D., Kadu, B. S., Mansara, P., Gupta, P., Deore, A. V., Chikate, R. C.and Kaul-Ghanekar, R. 2014. Synthesis, characterization and in vitro study of biocompatible cinnamaldehyde functionalized magnetite nanoparticles (CPGF Nps) for hyperthermia and drug delivery applications in breast cancer. PloS one, 9(9): e107315.
