بررسی مقایسه ای شیوع آلودگی تک یاخته ای آنتاموبا هیستولیتیکا در سگ های خانگی
محورهای موضوعی : بیماریهای دامهای کوچکبهنام پدرام 1 , رضا نقی ها نجف آبادی 2 , محمد محبی 3 , زهرا صادقیان 4 , فاطمه همایون 5
1 - گروه دامپزشکی، دانشکده کشاورزی و دامپزشکی ، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر ایران
2 - گروه علوم دامی دانشکده کشاورزی، دانشگاه یاسوج، یاسوج، ایران
3 - دانش آموخته عمومی دامپزشکی، دانشگاه آزاد اسلامی واحد شوشتر، شوشتر، ایران
4 - دانشجوی دکتری عمومی دامپزشکی، دانشگاه آزاد اسلامی واحد شوشتر، شوشتر، ایران
5 - دانشجوی دکتری عمومی دامپزشکی، دانشگاه آزاد اسلامی واحد شوشتر، شوشتر، ایران
کلید واژه: آنتاموبا هیستولیتیکا, سگ خانگی, انگل, آلودگی تک‌یاخته‌ای, PCR,
چکیده مقاله :
با افزایش تعامل انسان و حیوانات خانگی، به‌ویژه سگ‌ها، بررسی بیماریهای مشترک بین آن‌ها اهمیت روزافزونی یافته است. از جمله این بیماری‌ها، آلودگی به Entamoeba histolytica به عنوان یکی از عوامل مهم عفونت‌های تک‌یاخته‌ای، مورد توجه ویژه قرار گرفته است. مطالعه حاضر با هدف ارزیابی و مقایسه میزان شیوع این آلودگی در سگ‌های خانگی، با بهره‌گیری از دو روش گسترش مستقیم و واکنش زنجیره‌ای پلیمراز (PCR) انجام شد. در این تحقیق، ۲۵ نمونه مدفوع از سگ‌های خانگی با سنین و جنسیت متفاوت جمع‌آوری و از نظر حضور انگل مورد بررسی قرار گرفتند. نتایج حاصل از بررسی میکروسکوپی نشان داد که ۷ نمونه مشکوک به آلودگی بودند، اما آزمون PCR اختلاف معناداری میان نمونه‌های مشکوک و سایر نمونه‌ها گزارش نکرد. یافته‌ها حاکی از آن است که شیوع آلودگی به E.histolytica در جمعیت مورد مطالعه قابل توجه نبوده و روش PCR، به‌عنوان روشی دقیق‌تر و اختصاصی‌تر، می‌تواند در تشخیص مطمئن‌تر این انگل مؤثر واقع شود. نتایج این پژوهش می‌تواند در بهبود راهکارهای پیشگیری، تشخیص و کنترل بیماریهای زئونوز مفید باشد.
With the increasing interaction between humans and domestic animals, especially dogs, the investigation of zoonotic diseases has gained growing importance. Among these diseases, infection with Entamoeba histolytica as one of the significant causes of protozoan infections has received special attention. The present study was conducted with the aim of evaluating and comparing the prevalence of this infection in domestic dogs using two methods: direct smear and polymerase chain reaction (PCR). In this study, 25 fecal samples were collected from domestic dogs of different ages and sexes and examined for the presence of the parasite. Microscopic examination showed that 7 samples were suspected of infection; however, the PCR test did not report any significant difference between the suspected samples and other samples. The findings indicate that the prevalence of E. histolytica infection in the studied population was not considerable, and PCR, as a more precise and specific method, can be effective in the more reliable diagnosis of this parasite. The results of this research can be useful in improving strategies for prevention, diagnosis, and control of zoonotic diseases.
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