اثر 8 هفته تمرین تناوبی شدید بر بیان ژن GLP-1 در بافت پانکراس و عملکرد سلول های بتا در رت های چاق دیابتی نوع 2
محورهای موضوعی : فعالیت بدنی و تندرستیداود خورشیدی 1 , مهرداد نوری خواه 2
1 - دانشگاه آزاد واحد ساوه
2 - گروه تربیت بدنی و علوم ورزشی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران
کلید واژه: تمرین تناوبی, دیابت نوع 2, عملکرد سلول های بتا, اینکرتین,
چکیده مقاله :
هدف: پپتید شبه گلوکاگن-1 (GLP-1) یک هورمون اینکرتین است که در پاتوژنز و درمان دیابت نوع 2 نقش دارد. هدف این مطالعه بررسی تاثیر 8 هفته تمرین تناوبی شدید بر بیان ژن GLP-1 در بافت پانکراس رت های دیابتی نوع 2 بود.
روش: در این مطالعه تجربی14 سر رت نر نژاد ویستار با تزریق نیکوتین آمید-استرپتوزوتوسین به دیابت نوع 2 مبتلا شدند و تصادفی در دو گروه تمرین (7=n) و کنترل (7=n) قرار گرفتند. گروه تمرین، برنامه تمرینات تناوبی شدید را به مدت8 هفته و 5 روز در هفته با دویدن روی تردمیل اجرا کردند. 48 ساعت پس از آخرین جلسه تمرینی، بیان ژن GLP-1 در پانکراس، سطح گلوکز خون، انسولین و عملکرد سلول های بتا در هر دو گروه اندازه گیری شدند. تجزیه و تحلیل داده ها با استفاده از آزمون تی مستقل در سطح P< 0.05 انجام شد.
یافته ها:پس از مداخله ورزشی، بیان ژنGLP-1 ، سطح انسولین سرم و عملکرد سلول های بتا در گروه تمرین نسبت به گروه کنترل به میزان معنی داری بالاتر بودند (P<0.05). سطح گلوکز خون در گروه تمرین به میزان معنی داری پایین تر از گروه کنترل بود (P<0.05).
نتیجه گیری:بر اساس این یافته ها، به نظر می رسد بهبود گلوکز خون و عملکرد سلول های بتا در پاسخ به تمرینات تناوبی شدید تا اندازه ای به افزایش بیان ژن GLP-1 در پانکراس وابسته است.
Aim: Glucagon-like peptide-1 (GLP-1) is an incretin hormone that plays a role in the pathogenesis and treatment of type 2 diabetes mellitus (T2DM). The purpose of this study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) on GLP-1 expression in pancreatic tissue in T2DM rats.
Method: In this experimental study, fourteen males Wistar rats with T2DM induced by streptozotocin-nicotinamide injection were randomly divided into exercise (n=7) and control (n=7). The exercise group performed the HIIT protocol on a treadmill for 8 weeks and 5 days per week. GLP1 expression in pancreatic tissue, blood glucose level, insulin and beta cell function were measured 48 hours after the last training session. Data were analyzed by independent T test at P< 0.05.
Results: After the exercise intervention, GLP-1 expression in pancreatic tissue, serum insulin and beta cell function were significantly higher in the exercise group compared to the control group (P˂0.05). Blood glucose levels were significantly lower in the exercise group than in the control group (P˂0.05).
Conclusion: Based on these findings, it appears that the improvement in blood glucose and beta cell function in response to HIIT are partially dependent on increased GLP-1 expression in the pancreatic tissue.
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