From plant to nanoparticle: Evaluating the biological efficacy of Cannabis sativa L. methanol extract, unlocking the bioapotential of Cannabis sativa L. in the green fabrication of silver nanoparticles and their characterization
محورهای موضوعی : Medicinal and Herbal PlantsMajid Mohammadhosseini 1 , Behnam Mahdavi 2 , Mohammad Reza Kianasab 3
1 - Department of Chemistry and Biochemistry, Sha.C., Islamic Azad University, Shahrood, Iran
2 - Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
3 - Department of Chemistry and Biochemistry, Sha.C., Islamic Azad University, Shahrood, Iran
کلید واژه: Biological activities, Cannabaceae family, Cannabis sativa L., Characterization, Green synthesis, Methanol extract, Silver nanoparticles (AgNPS),
چکیده مقاله :
Cannabis sativa L. (CSL) is a versatile plant recognized for its medicinal, industrial, and nutritional applications. Rich in bioactive compounds, it has been extensively studied for its phytochemical properties and biological activities. The current report focuses on the preparation of a methanol extract from the stems, flowers, and leaves of this plant, along with an assessment of its relevant biological activities, including antioxidant, antibacterial, and antifungal properties. In the second phase of this study, the green synthesis of Ag nanoparticles was successfully conducted using a methanolic extract of Cannabis sativa L. (MECS). In this process, CSL serves as a natural reducing and capping agent. The synthesized nanoparticles were subsequently characterized using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). This method offers a sustainable and environmentally-friendly approach to nanoparticle production.
Cannabis sativa L. (CSL) is a versatile plant recognized for its medicinal, industrial, and nutritional applications. Rich in bioactive compounds, it has been extensively studied for its phytochemical properties and biological activities. The current report focuses on the preparation of a methanol extract from the stems, flowers, and leaves of this plant, along with an assessment of its relevant biological activities, including antioxidant, antibacterial, and antifungal properties. In the second phase of this study, the green synthesis of Ag nanoparticles was successfully conducted using a methanolic extract of Cannabis sativa L. (MECS). In this process, CSL serves as a natural reducing and capping agent. The synthesized nanoparticles were subsequently characterized using Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). This method offers a sustainable and environmentally-friendly approach to nanoparticle production.
Ahidar, N., Labhar, A., Benamari, O., Ahari, M., Salhi, A., Elyoussfi, A., Amhamdi, H., 2024. Phenolic content and antioxidant activity of Cannabis sativa L. flowers from the Ketama region in Northern Morocco. Ecol. Eng. Environ. Tech. 25(1), 209-215.
Ahmadi, F., Lackner, M., 2024. Green synthesis of silver nanoparticles from Cannabis sativa: Properties, synthesis, mechanistic aspects, and applications. ChemEngineering 8(4), 64.
Ahmed, M., Ji, M., Qin, P., Gu, Z., Liu, Y., Sikandar, A., Iqbal, M.F., Javeed, A., 2019. Phytochemical screening, total phenolic and flavonoids contents and antioxidant activities of Citrullus colocynthis L. and Cannabis sativa L. Appl. Ecol. Environ. Res. 17(3), 6961-6979.
Ahmed, S., Ikram, S., 2015. Silver nanoparticles: One pot green synthesis using Terminalia arjuna extract for biological application. J. Nanomed. Nanotechnol. 6(4), 1-6.
Ali, M.H., Azad, M.A.K., Khan, K.A., Rahman, M.O., Chakma, U., Kumer, A., 2023. Analysis of crystallographic structures and properties of silver nanoparticles synthesized using PKL extract and nanoscale characterization techniques. ACS Omega 8(31), 28133-28142.
Amalia, N., Okta, F.N., Zahra, A.A., Nuari, D.A., 2024. Update review: Extraction, purification, and pharmacological activities of Gotu Kola terpenoids. Lett. Appl. NanoBioSci. 13(1), 1-17.
Ananthi, P., Jeyapaul, U., Anand, A.J.B., Kala, S.M.J., 2016. Green synthesis and characterization of silver nanoparticles using Triumfetta rotundifolia plant extract and its antibacterial activities. J. Nat. Prod. Plant Resour. 6(3), 21-27.
Arabkhani, P., Asfaram, A., Aghaei-Jazeh, M., Ateia, M., 2022. Plant-mediated green synthesis of nanocomposite-based multifunctional adsorbent with antibacterial activity and high removal efficiency of micropollutants from contaminated waters. J. Water Process Eng. 49, 103025.
Arora, R., Babbar, R., Kaur, R., Rana, P., 2023. An Insight into the Potential of Natural Products as Anti-inflammatory Agents A Systematic Review, Manufacturing Technologies and Production Systems: Principles and Practices. CRC Press, pp. 152-162.
Asif, M., Yasmin, R., Asif, R., Ambreen, A., Mustafa, M., Umbreen, S., 2022. Green synthesis of silver nanoparticles (AgNPs), structural characterization, and their antibacterial potential. Dose Response 20(2), 15593258221088709.
Banerjee, R., Erridge, S., Salazar, O., Mangal, N., Couch, D., Pacchetti, B., Sodergren, M.H., 2022. Real world evidence in medical Cannabis research. Ther. Innov. Regul. Sci. 56(1), 8-14.
Bashir, T., Qureshi, M.Z., 2015. Phytosynthesis of silver nanoparticles using E. camaldulensis leaf extract and their characterization. J. Chil. Chem. Soc. 60(1), 2861-2863.
Bautista, J.L., Yu, S., Tian, L., 2021. Flavonoids in Cannabis sativa: Biosynthesis, bioactivities, and biotechnology. ACS Omega 6(8), 5119-5123.
Bawazeer, S., 2024. Green synthesis of silver nanoparticles from Euphorbia milii plant extract for enhanced antibacterial and enzyme inhibition effects. Int. J. Health Sci. 18(2), 25.
Bawazeer, S., Rauf, A., Rahman, K.U., Ali, J., Uddin, G., Begum, F., Mubarak, M.S., Ramadan, M.F., 2020. Green synthesis and antimicrobial potential of silver/gold nanoparticles functionalized with Debregeasia salicifolia D. Don. J. Pure Appl. Microbiol. 14(4), 2513-2523.
Blainski, A., Lopes, G.C., De Mello, J.C.P., 2013. Application and analysis of the Folin Ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules 18(6), 6852-6865.
Blaskovich, M.A.T., Kavanagh, A.M., Elliott, A.G., Zhang, B., Ramu, S., Amado, M., Lowe, G.J., Hinton, A.O., Pham, D.M.T., Zuegg, J., Beare, N., Quach, D., Sharp, M.D., Pogliano, J., Rogers, A.P., Lyras, D., Tan, L., West, N.P., Crawford, D.W., Peterson, M.L., Callahan, M., Thurn, M., 2021. The antimicrobial potential of cannabidiol. Commun. Biolog. 4(1), 7.
Bowe, A., Kerr, P.L., 2024. Endogenous Opioid Activity as the Mechanism of Action for Mitragyna speciosa (Kratom): The Current State of the Evidence, Advances in Neurobiology. Springer, pp. 287-313.
Bukowska, B., 2024. Current and potential use of biologically active compounds derived from Cannabis sativa L. in the treatment of selected diseases. Int. J. Mol. Sci. 25(23), 12738.
Cásedas, G., Moliner, C., Maggi, F., Mazzara, E., López, V., 2022. Evaluation of two different Cannabis sativa L. extracts as antioxidant and neuroprotective agents. Front. Pharmacol. 13, 1009868.
Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M.H., Elsohly, M.A., Khan, I.A., 2014. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: A comparative study. Evid. Based Complement. Alternat. Med. 2014, 253875.
Chandraker, S.K., Ghosh, M.K., Lal, M., Shukla, R., 2021. A review on plant-mediated synthesis of silver nanoparticles, their characterization and applications. Nano. Express. 2(2), 022008.
Chen, L., Li, H.L., Zhou, H.J., Zhang, G.Z., Zhang, Y., Wang, Y.M., Wang, M.Y., Yang, H., Gao, W., 2024. Feature-based molecular network-assisted cannabinoid and flavonoid profiling of Cannabis sativa leaves and their antioxidant properties. Antioxidants 13(6), 749.
Coronado-Aceves, E.W., Sánchez-Escalante, J.J., López-Cervantes, J., Robles-Zepeda, R.E., Velázquez, C., Sánchez-Machado, D.I., Garibay-Escobar, A., 2016. Antimycobacterial activity of medicinal plants used by the Mayo people of Sonora, Mexico. J. Ethnopharmacol. 190, 106-115.
Demarchi, C.A., Cruz, A.B., da Silva Bitencourt, C.M., Farias, I.V., Ślawska-Waniewska, A., Nedelko, N., Dłużewski, P., Morawiec, K., Calisto, J.F.F., Martello, R., Dal Magro, J., Rodrigues, C.A., 2020. Eugenia umbelliflora mediated reduction of silver nanoparticles incorporated into O-carboxymethylchitosan/y-Fe2O3: Synthesis, antimicrobial activity and toxicity. Int. J. Biol. Macromol. 155, 614-624.
Eid, M.M., 2022. Characterization of Nanoparticles by FTIR and FTIR-Microscopy, Handbook of Consumer Nanoproducts. Springer Nature, pp. 645-673.
Elumalai, D., Hemavathi, M., Deepaa, C.V., Kaleena, P.K., 2017. Evaluation of phytosynthesised silver nanoparticles from leaf extracts of Leucas aspera and Hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors. Parasite Epidemiol. Control 2(4), 15-26.
Flores-Sanchez, I.J., Verpoorte, R., 2008. PKS activities and biosynthesis of cannabinoids and flavonoids in Cannabis sativa L. plants. Plant Cell Physiol. 49(12), 1767-1782.
Fordjour, E., Manful, C.F., Sey, A.A., Javed, R., Pham, T.H., Thomas, R., Cheema, M., 2023. Cannabis: A multifaceted plant with endless potentials. Front. Pharmacol. 14, 1200269.
Giardina, A., Palmieri, R., Ponticelli, M., Antonelli, C., Carlucci, V., Colangelo, M., Benedetto, N., Di Fazio, A., Milella, L., 2024. Is a low dosage of medical Cannabis effective for treating pain related to fibromyalgia? A pilot study and systematic review. J. Clin. Med. 13(14), 4088.
Giri, A.K., Jena, B., Biswal, B., Pradhan, A.K., Arakha, M., Acharya, S., Acharya, L., 2022. Green synthesis and characterization of silver nanoparticles using Eugenia roxburghii DC. extract and activity against biofilm-producing bacteria. Sci. Rep. 12(1), 8383.
Goudarzi, M., Mir, N., Mousavi-Kamazani, M., Bagheri, S., Salavati-Niasari, M., 2016. Biosynthesis and characterization of silver nanoparticles prepared from two novel natural precursors by facile thermal decomposition methods. Sci. Rep. 6(1), 32539.
Gulcin, İ., Alwasel, S.H., 2023. DPPH radical scavenging assay. Process. 11(8), 2248.
Hourfane, S., Mechqoq, H., Bekkali, A.Y., Rocha, J.M., El Aouad, N., 2023. A comprehensive review on Cannabis sativa ethnobotany, phytochemistry, molecular docking and biological activities. Plants 12(6), 1245.
Huang, J., Li, Q., Sun, D., Lu, Y., Su, Y., Yang, X., Wang, H., Wang, Y., Shao, W., He, N., Hong, J., Chen, C., 2007. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology 18(10), 105104.
Huang, M., Liu, S., Gong, S., Xu, P., Yang, K., Chen, S., Wang, C., Chen, Q., 2019. Silver nanoparticles encapsulated in an N‑doped porous carbon matrix as high-active catalysts toward oxygen reduction reaction via electron transfer to outer graphene shells. ACS Sustainable Chem. Eng. 7(19), 16511-16519.
Iravani, S., 2011. Green synthesis of metal nanoparticles using plants. Green Chem. 13(10), 2638-2650.
Izzo, L., Castaldo, L., Narváez, A., Graziani, G., Gaspari, A., Rodríguez-Carrasco, Y., Ritieni, A., 2020. Analysis of phenolic compounds in commercial Cannabis sativa L. inflorescences using UHPLC-Q-orbitrap HRMs. Molecules 25(3), 631.
Jemal, K., Sandeep, B.V., Pola, S., 2017. Synthesis, characterization, and evaluation of the antibacterial activity of Allophylus serratus leaf and leaf derived callus extracts mediated silver nanoparticles. J. Nanomater. 2017(1), 4213275.
Jin, D., Dai, K., Xie, Z., Chen, J., 2020. Secondary metabolites profiled in Cannabis inflorescences, leaves, stem barks, and roots for medicinal purposes. Sci. Rep. 10(1), 3309.
Kalinowska, M., Płońska, A., Trusiak, M., Gołębiewska, E., Gorlewska-Pietluszenko, A., 2022. Comparing the extraction methods, chemical composition, phenolic contents and antioxidant activity of edible oils from Cannabis sativa and Silybum marianu seeds. Sci. Rep. 12(1), 20609.
Kannan, S.K., Sundrarajan, M., 2015. Green synthesis of ruthenium oxide nanoparticles: Characterization and its antibacterial activity. Adv. Powder Technol. 26(6), 1505-1511.
Kianasab, M.R., Mohammadhosseini, M., Nekoei, M., Mahdavi, B., Baheri, T., 2023. GC/MS analysis of the hydrodistilled essential oils and volatiles from the aerial parts of Cannabis sativa L. Nat. Prod. Res., 1-5.
Kim, S.M., Choi, H.J., Lim, J.A., Woo, M.A., Chang, H.J., Lee, N., Lim, M.C., 2023. Biosynthesis of silver nanoparticles from Duchesnea indica extracts using different solvents and their antibacterial activity. Microorganisms 11(6), 1539.
Kosiba, J.D., Maisto, S.A., Ditre, J.W., 2019. Patient-reported use of medical Cannabis for pain, anxiety, and depression symptoms: Systematic review and meta-analysis. Soc. Sci. Med. 233, 181-192.
Kurapati, S., Srivastava, P., 2018. Application of Debye-Scherrer formula in the determination of silver nano particles shape. Int. J. Manag. Technol. Eng. 8(7), 81-84.
Lapierre, É., Monthony, A.S., Torkamaneh, D., 2023. Genomics-based taxonomy to clarify Cannabis classification. Genome 66(08), 202-211.
Lawag, I.L., Nolden, E.S., Schaper, A.A.M., Lim, L.Y., Locher, C., 2023. A modified Folin-Ciocalteu assay for the determination of total phenolics content in honey. Appl. Sci. 13(4), 2135.
Leinen, Z.J., Mohan, R., Premadasa, L.S., Acharya, A., Mohan, M., Byrareddy, S.N., 2023. Therapeutic potential of Cannabis: A comprehensive review of current and future applications. Biomedicines 11(10), 2630.
Lentz, D.L., Hamilton, T.L., Meyers, S.A., Dunning, N.P., Reese-Taylor, K., Hernández, A.A., Walker, D.S., Tepe, E.J., Esquivel, A.F., Weiss, A.A., 2024. Psychoactive and other ceremonial plants from a 2,000-year-old Maya ritual deposit at Yaxnohcah, Mexico. PLOS ONE 19(4), e0301497.
Li, M., Qian, M., Jiang, Q., Tan, B., Yin, Y., Han, X., 2023. Evidence of flavonoids on disease prevention. Antioxidants 12(2), 527.
Liu, Y., Xiao, A.P., Cheng, H., Liu, L.L., Kong, K.W., Liu, H.Y., Wu, D.T., Li, H.B., Gan, R.Y., 2022. Phytochemical differences of hemp (Cannabis sativa L.) leaves from different germplasms and their regulatory effects on lipopolysaccharide-induced inflammation in Matin-Darby canine kidney cell lines. Front. Nutr. 9, 902625.
Luz-Veiga, M., Amorim, M., Pinto-Ribeiro, I., Oliveira, A.L.S., Silva, S., Pimentel, L.L., Rodríguez-Alcalá, L.M., Madureira, R., Pintado, M., Azevedo-Silva, J., Fernandes, J., 2023. Cannabidiol and cannabigerol exert antimicrobial activity without compromising skin microbiota. Int. J. Mol. Sci. 24(3), 2389.
Mahdavi, B., Mohammadhosseini, M., 2022. Antioxidant, antimicrobial and anti-prostate cancer activity of the extracts from different parts of Etlingera velutina (Ridl.) R. M. Sm (Zingiberaceae). Trends Phytochem. Res. 6(4), 353-362.
Mandal, S., Marpu, S.B., Hughes, R., Omary, M.A., Shi, S.Q., 2021. Green synthesis of silver nanoparticles using Cannabis sativa extracts and their anti-bacterial activity. Green Sustain. Chem. 11(1), 38-48.
Mathur, N.K., Ruhm, C.J., 2023. Marijuana legalization and opioid deaths. J. Health Econ. 88, 102728.
Matos, C., Pereira, A.T., Dias, M.J., Sousa, C., Vinha, A.F., Moutinho, C., Carvalho, M., 2025. Cannabis for chronic pain: Mechanistic insights and therapeutic challenges. Stresses 5(1), 7.
Meena, P.R., Singh, A.P., Tejavath, K.K., 2020. Biosynthesis of silver nanoparticles using Cucumis prophetarum aqueous leaf extract and their antibacterial and antiproliferative activity against cancer cell lines. ACS Omega 5(10), 5520-5528.
Mirzamohammad, E., Alirezalu, A., Alirezalu, K., Norozi, A., Ansari, A., 2021. Improvement of the antioxidant activity, phytochemicals, and cannabinoid compounds of Cannabis sativa by salicylic acid elicitor. Food Sci. Nutr. 9(12), 6873-6881.
Mishra, K., Ojha, H., Chaudhury, N.K., 2012. Estimation of antiradical properties of antioxidants using DPPH center dot assay: A critical review and results. Food Chem. 130(4), 1036-1043.
Mittal, A.K., Chisti, Y., Banerjee, U.C., 2013. Synthesis of metallic nanoparticles using plant extracts. Biotechnol. Adv. 31(2), 346-356.
Mohammadhosseini, M., Jeszka-Skowron, M., 2023. A systematic review on the ethnobotany, essential oils, bioactive compounds, and biological activities of Tanacetum species. Trends Phytochem. Res. 7(1), 1-29.
Moond, M., Singh, S., Sangwan, S., Rani, S., Beniwal, A., Rani, J., Kumari, A., Rani, I., Devi, P., 2023. Phytofabrication of silver nanoparticles using Trigonella foenum-graceum L. leaf and evaluation of its antimicrobial and antioxidant activities. Int. J. Mol. Sci. 24(4), 3480.
Motiejauskaitė, D., Ullah, S., Kundrotaitė, A., Žvirdauskienė, R., Bakšinskaitė, A., Barčauskaitė, K., 2023. Isolation of biologically active compounds from Cannabis sativa L. inflorescences by using different extraction solvents and evaluation of antimicrobial activity. Antioxidants 12(5), 998.
Murti, Y., Singh, S., Pathak, K., 2024. Classical Techniques for Extracting Essential Oils from Plants, Essential Oils: Extraction Methods and Applications. Wiley, pp. 795-858.
Ndlovu, S.B., Naidoo, D., van Staden, J., Gebashe, F.C., 2024. A systematic review of Cannabis sativa L. cultivation techniques: A comprehensive overview of tissue culture innovations and growth optimization. Ind. Crops Prod. 222, 119539.
Perlman, A.I., McLeod, H.M., Ventresca, E.C., Salinas, M.G., Post, P.J., Schuh, M.J., Abu Dabrh, A.M., 2021. Medical Cannabis state and federal regulations: Implications for United States health care entities. Mayo Clin. Proc. 96(10), 2671-2681.
Pradeep, M., Kruszka, D., Kachlicki, P., Mondal, D., Franklin, G., 2022. Uncovering the phytochemical basis and the mechanism of plant extract-mediated eco-friendly synthesis of silver nanoparticles using ultra-performance liquid chromatography coupled with a photodiode array and high-resolution mass spectrometry. ACS Sustainable Chem. Eng. 10(1), 562-571.
Rajasekar, A., Moorthi, M., Deivasigamani, P., Sekar, S., Amar, G., 2023. A Review on Screening, Isolation, and Characterization of Phytochemicals in Plant Materials: Methods and Techniques, Pharmacological Benefits of Natural Agents. IGI Global, pp. 1-12.
Rajeshkumar, S., Malarkodi, C., Kumar, V., 2017. Synthesis and characterization of silver nanoparticles from marine brown seaweed and its antifungal efficiency against clinical fungal pathogens. Asian J. Pharm. Clin. Res., 190-193.
Ramos, R., Bezerra, I., Ferreira, M., Soares, L., 2017. Spectrophotometric quantification of flavonoids in herbal material, crude extract, and fractions from leaves of Eugenia uniflora Linn. Pharmacogn. Res. 9(3), 253-260.
Ranjan, R., Kumar, M., Prasad, S.M., 2023. XRD analysis of nanoparticles synthesized using aqueous and alcoholic extracts of Cuscuta reflexa. Balneo. PRM. Res. J. 14(3), 585.
Ransing, R., de la Rosa, P.A., Pereira-Sanchez, V., Handuleh, J.I.M., Jerotic, S., Gupta, A.K., Karaliuniene, R., Filippis, R., Peyron, E., Güngör, E.S., Boujraf, S., Yee, A., Vahdani, B., Shoib, S., Stowe, M.J., Jaguga, F., Dannatt, L., Silva, A.K.D., Grandinetti, P., Jatchavala, C., 2022. Current state of Cannabis use, policies, and research across sixteen countries: Cross-country comparisons and international perspectives. Trends 44, e20210263.
Rehm, J., Elton-Marshall, T., Sornpaisarn, B., Manthey, J., 2019. Medical marijuana. What can we learn from the experiences in Canada, Germany and Thailand? Int. J. Drug Policy 74, 47-51.
Santhoshkumar, R., Hima Parvathy, A., Soniya, E.V., 2021. Phytosynthesis of silver nanoparticles from aqueous leaf extracts of Piper colubrinum: Characterisation and catalytic activity. J. Exp. Nanosci. 16(1), 295-309.
Santra, T.S., Tseng, F.-G.K., Barik, T.K., 2014. Biosynthesis of silver and gold nanoparticles for potential biomedical applications—A brief review. J. Nanopharm. Drug Deliv. 2(4), 249-265.
Sarkar, S., Das, R., 2018. Shape effect on the elastic properties of Ag nanocrystals. Micro Nano Lett. 13(3), 312-315.
Sharif, N., Jabeen, H., 2024. Natural sources for coumarins and their derivatives with relevance to health-promoting properties: A systematic review. Trends Phytochem. Res. 8(3), 149-162.
Shraim, A.M., Ahmed, T.A., Rahman, M.M., Hijji, Y.M., 2021. Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. LWT 150, 111932.
Singh, A., Singh, D., Sharma, S., Mittal, N., 2023. A review on biosynthesis, regulation, and applications of terpenes and terpenoids. Trends Phytochem. Res. 7(4), 228-245.
Stasiłowicz-Krzemień, A., Sip, S., Szulc, P., Walkowiak, J., Cielecka-Piontek, J., 2023. The antioxidant and neuroprotective potential of leaves and inflorescences extracts of selected hemp varieties obtained with scCO2. Antioxidants 12(10), 1827.
Steel, L., Welling, M., Ristevski, N., Johnson, K., Gendall, A., 2023. Comparative genomics of flowering behavior in Cannabis sativa. Front. Plant Sci. 14, 1227898.
Stozhko, N., Tarasov, A., Tamoshenko, V., Bukharinova, M., Khamzina, E., Kolotygina, V., 2024. Green silver nanoparticles: Plant-extract-mediated synthesis, optical and electrochemical properties. Physchem. 4(4), 402-419.
Suman, S., Loveleen, L., Bhandari, M., Syed, A., Bahkali, A.H., Manchanda, R., Nimesh, S., 2022. Antibacterial, antioxidant, and haemolytic potential of silver nanoparticles biosynthesized using roots extract of Cannabis sativa plant. Artif. Cells Nanomed. Biotechnol. 50(1), 343-351.
Tiago, F.J., Paiva, A., Matias, A.A., Duarte, A.R.C., 2022. Extraction of bioactive compounds from Cannabis sativa L. flowers and/or leaves using deep eutectic solvents. Front. Nutr. 9, 892314.
Torres, C.A., Zampini, I.C., Nunez, M.B., Isla, M.I., Castro, M.P., Gonzalez, A.M., 2013. In vitro antimicrobial activity of 20 selected climber species from the Bignoniaceae family. Nat. Prod. Res. 27(22), 2144-2148.
Urnukhsaikhan, E., Bold, B.E., Gunbileg, A., Sukhbaatar, N., Mishig-Ochir, T., 2021. Antibacterial activity and characteristics of silver nanoparticles biosynthesized from Carduus crispus. Sci. Rep. 11(1), 21047.
Vanlalveni, C., Lallianrawna, S., Biswas, A., Selvaraj, M., Changmai, B., Rokhum, S.L., 2021. Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: A review of recent literature. RSC Adv. 11(5), 2804-2837.
Velgosova, O., Dolinská, S., Podolská, H., Mačák, L., Čižmárová, E., 2024. Impact of plant extract phytochemicals on the synthesis of silver nanoparticles. Materials 17(10), 2252.
Wan Mat Khalir, W.K.A., Shameli, K., Jazayeri, S.D., Othman, N.A., Che Jusoh, N.W., Hassan, N.M., 2020. Biosynthesized silver nanoparticles by aqueous stem extract of Entada spiralis and screening of their biomedical activity. Front. Chem. 8, 620.
Werner, M., Bereswill, S., Heimesaat, M.M., 2024. A recent update on the antibacterial effects of distinct bioactive molecules derived from the Cannabis plant. Eur. J. Microbiol. Immunol. 14(4), 333-339.
Wilson, J., Langcake, A., Bryant, Z., Freeman, T.P., Leung, J., Chan, G.C., Englund, A., Graham, M., Stockings, E., 2025. The safety and efficacy of cannabinoids for the treatment of mental health and substance use disorders: Protocol for a systematic review and meta-analysis. Syst. Rev. 14(1), 1-12.
Wright, P., Walsh, Z., Margolese, S., Sanchez, T., Arlt, S., Belle-Isle, L., St.pierre, M., Bell, A., Daeninck, P., Gagnon, M., Lacasse, G., MacCallum, C., Mandarino, E., Yale, J., O'Hara, J., Costiniuk, C., 2020. Canadian clinical practice guidelines for the use of plant-based Cannabis and cannabinoid-based products in the management of chronic non-cancer pain and co-occurring conditions: Protocol for a systematic literature review. BMJ Open 10(5), e036114.
Xiang, G., Guo, S., Qin, J., Gao, H., Zhang, Y., Wang, S., 2024. Comprehensive insight into the pharmacology, pharmacokinetics, toxicity, detoxification and extraction of hypaconitine from Aconitum plants. J. Ethnopharmacol. 321, 117505.
Xu, J., Hu, J., Peng, C., Liu, H., Hu, Y., 2006. A simple approach to the synthesis of silver nanowires by hydrothermal process in the presence of gemini surfactant. J. Colloid Interface Sci. 298(2), 689-693.
Zhang, Y., Mahdavi, B., Mohammadhosseini, M., Rezaei-Seresht, E., Paydarfard, S., Qorbani, M., Karimian, M., Abbasi, N., Ghaneialvar, H., Karimi, E., 2021. Green synthesis of NiO nanoparticles using Calendula officinalis extract: Chemical charactrization, antioxidant, cytotoxicity, and anti-esophageal carcinoma properties. Arabian J. Chem. 14, 103105.