Anti-depressant effect of Oliveria Decumbens in gonadectomized rat
محورهای موضوعی : فیزیولوژی
Siyavash Moshafi
1
,
Shahin Hassanpour
2
,
Ahmad Asghari
3
,
Parviz Tajik
4
1 - Faculty of Veterinary Medicine, SR.C., Islamic Azad University, Tehran, Iran
2 - Department of Veterinary Basic Sciences, SR.C., Islamic Azad University, Tehran, Iran
3 - Department of Veterinary Clinical Sciences, SR.C., Islamic Azad University, Tehran, Iran
4 - Department of Veterinary Clinical Sciences, SR.C., Islamic Azad University, Tehran, Iran
کلید واژه: Antidepressant, Oliveria decumbens, Gonadectomy, Rat,
چکیده مقاله :
This study aimed to determine the antidepressant effect of Oliveria Decumbens extract (ODE) in gonadectomized rats. Thirty male rats were allocated into 6 experimental groups. The control group underwent identical surgeries, but the testes were not clamped, ligated, or excised. In the imipramine group, castration was identical, followed by imipramine administration (5 mg/kg) for 2 weeks. In groups 4, 5, and 6, following castration, rats were p.o. injected with ODE (100, 200, and 400 mg/kg) for 2 weeks, respectively. Then, the forced swimming test (FST), the tail suspension test (TST), and the open field test (OFT) were done. Also, serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and total antioxidant status (TAS) were determined. According to the data, immobility time using FST and TST significantly increased in the gonadectomized rat (p< 0.05). Administration of imipramine significantly decreased immobility time using FST and TST (p< 0.05). A dose-dependent decrease in immobility time in the FST and TST was observed in gonadectomized rats treated with ODE (200 and 400 mg/kg) compared with the control group (P<0.05). Castration significantly decreased the number of crosses in OFT, and administration of imipramine or treatment with ODE (200 and 400 mg/kg) significantly increased it (p< 0.05). Serum MDA levels significantly increased, while SOD, GPx, and TAS levels decreased in gonadectomized rats compared with the control group (p< 0.05). Administration of the ODE (200 and 400 mg/kg) significantly decreased serum MDA while increasing SOD, GPx, and TAS levels (p< 0.05). These results suggest that ODE has an antidepressant effect in gonadectomized rats, mediated by its antioxidant activity.
This study aimed to determine the antidepressant effect of Oliveria Decumbens extract (ODE) in gonadectomized rats. Thirty male rats were allocated into 6 experimental groups. The control group underwent identical surgeries, but the testes were not clamped, ligated, or excised. In the imipramine group, castration was identical, followed by imipramine administration (5 mg/kg) for 2 weeks. In groups 4, 5, and 6, following castration, rats were p.o. injected with ODE (100, 200, and 400 mg/kg) for 2 weeks, respectively. Then, the forced swimming test (FST), the tail suspension test (TST), and the open field test (OFT) were done. Also, serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and total antioxidant status (TAS) were determined. According to the data, immobility time using FST and TST significantly increased in the gonadectomized rat (p< 0.05). Administration of imipramine significantly decreased immobility time using FST and TST (p< 0.05). A dose-dependent decrease in immobility time in the FST and TST was observed in gonadectomized rats treated with ODE (200 and 400 mg/kg) compared with the control group (P<0.05). Castration significantly decreased the number of crosses in OFT, and administration of imipramine or treatment with ODE (200 and 400 mg/kg) significantly increased it (p< 0.05). Serum MDA levels significantly increased, while SOD, GPx, and TAS levels decreased in gonadectomized rats compared with the control group (p< 0.05). Administration of the ODE (200 and 400 mg/kg) significantly decreased serum MDA while increasing SOD, GPx, and TAS levels (p< 0.05). These results suggest that ODE has an antidepressant effect in gonadectomized rats, mediated by its antioxidant activity.
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