اثر نانوکیتوزان حامل عصاره دانه گرده گل زنبورعسل بر سطح آنتی¬اکسیدان¬ها و پراکسیداسیون لیپیدی بافت کبد موش آلوده به استافیلوکوکوس اورئوس
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
زهرا بیابانی
1
,
زهرا کشتمند
2
1 - گروه زيست شناسي، واحد تهران مرکزي، دانشگاه آزاد اسلامي، تهران، ايران
2 - گروه‌زیست‌شناسی،دانشکده‌علوم‌پایه،واحد‌تهران‌مرکزی،دانشگاه‌آزاد‌اسلامی،تهران،ایران.
کلید واژه: نانوکیتوزان , دانه گرده گل, آنتی اکسیدان, پراکسیداسیون لیپید., کبد, استافیلوکوکوس اورئوس, موش صحرایی,
چکیده مقاله :
امروزه، استفاده از تکنولوژی و معرفی ترکیبات ضد باکتری دوستتدار محیط زیست مانند نانوحاملهای حاوی محصولات گیاهی مورد توجه است.هدف از این پژوهش بررسی اثر نانوکیتوزان حامل دانه گرده گل زنبورعسل بر تغییرات سطح آنتی اکسیدانها و پراکسیداسیون لیپید بافت کبد آلوده به باکتری استافیلوکوکوس اورئوس در موشهای صحرایی نر نژاد ویستار میباشد.در این مطالعه تجربی 28 موش صحرایی نر نژاد ویستار، درچهار گروه شامل گروه کنترل ،آلوده به استافیلوکوکوس اورئوس CFU /ml)108 )، دریافت کننده نانوکیتوزان(mg/kg100)و مدل آلوده دریافت کننده نانوکیتوزان حامل دانه گرده گل(mg/kg 100)تقسیم بندی شدند. القا آلودگی و دریافت نانوکیتوزان با روش گاواژ انجام شد. بعد از دوره تیمار و تشریح حیوانات، بافت کبد جهت بررسی سطح فعالیت آنتیاکسیدان تام ،سوپراکسیددسموتاز، گلوتاتیون پراکسیداز، کاتالاز و مالون دی آلدهیدگروههای مختلف استخراج شد. آنالیز دادهها در گروههای مختلف با نرم افزارSPSS و آزمون آماری واریانس یکطرفه انجام و05/0p< معنی‌دار در نظر گرفته شدنتایج حاصل، تغییر معنادار سطح آنتی اکسیدان تام،سوپراکسیددسموتاز، گلوتاتیون پراکسیداز، کاتالاز و مالوندیآلدهیدگروههای آلوده را در مقایسه با گروه کنترل نشان داد(P<0.05). در حالیکه کاهش سطح مالون دی آلدهید ، افزایش سطح آنتی اکسیدان تام، سوپراکسیددسموتاز، گلوتاتیون پراکسیداز و کاتالاز در گروههای تیمار در مقایسه با گروه آلوده به صورت معنادار نشان داده شد(P<0.05)نر اساس نتایج به دست آمده نانوکیتوزان حامل دانه گرده گل زنبورعسل اثر تعدیلکنندگی بر سطح آنتیاکسیدانها و پراکسیداسیون لیپید بافت کبد موشهای آلوده به باکتری را نشان داد. بنابراین احتمالا بتواند به عنوان یک راهکار امیدوارکننده درمانی استفاده شود
Today,the use of technology and the introduction of environmentally friendly antibacterial compounds such as nanocarriers containing plant products, is of interest.The aim of this study is to investigate the effect of nanochitosan carrying bee pollen on changes in antioxidant levels and lipid peroxidation in liver tissue infected with Staphylococcus aureus in male Wistar rats.In this experimental study,28male Wistar rats were divided into 4groups including control group, infected with Staphylococcus aureu(108 CFU/ml receiving nanochitosa(100mg/kg)and infected model+receiving nanochitosan carrying Bee polle(100mg/kg Infection induction and receiving nanochitosan were performed by gavage.After the treatment period and dissection of the animals, liver tissue was extracted to examine the activity levels of total antioxidant,superoxide dismutase, glutathione peroxidase,catalase, and malondialdehyde of different groups Data analysis in different groups was performed with SPSS software and one-way variance statistical test and p<0.05was considered significant.The results showed a significant change in the levels of total antioxidants,superoxide dismutase,glutathione peroxidase,catalase and malondialdehyde in the infected groups compared to the control group.While a decrease in the level of malondialdehyde and an increase in the levels of total antioxidants, superoxide dismutase, glutathione peroxidase and catalase in the treatment groups compared to the infected group were significantly show.Based on the results obtained,nanochitosan carrying bee pollen showed a modulating effect on the levels of antioxidants and lipid peroxidation in the liver tissue of ratinfected with bacteria.Therefore,it can probably be used as a promising therapeutic strategy
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