Effect of nanochitosan carrying bee pollen extract on antioxidant levels and lipid peroxidation in liver tissue of mice infected with Staphylococcus aureu
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Zahra Biabani
1
,
زهرا کشتمند
2
1 - Departmant of Biology,Central Tehran Branch, Islamic Azad Univercity, Tehran, Iran
2 -
Keywords: nanochitosan, Bee pollen, antioxidant, lipid peroxidation, liver, Staphylococcus aureus, rat,
Abstract :
Today,the use of technology and the introduction of environmentally friendly antibacterial compounds such as nanocarriers containing plant products, is of interest.The aim of this study is to investigate the effect of nanochitosan carrying bee pollen on changes in antioxidant levels and lipid peroxidation in liver tissue infected with Staphylococcus aureus in male Wistar rats.In this experimental study,28male Wistar rats were divided into 4groups including control group, infected with Staphylococcus aureu(108 CFU/ml receiving nanochitosa(100mg/kg)and infected model+receiving nanochitosan carrying Bee polle(100mg/kg Infection induction and receiving nanochitosan were performed by gavage.After the treatment period and dissection of the animals, liver tissue was extracted to examine the activity levels of total antioxidant,superoxide dismutase, glutathione peroxidase,catalase, and malondialdehyde of different groups Data analysis in different groups was performed with SPSS software and one-way variance statistical test and p<0.05was considered significant.The results showed a significant change in the levels of total antioxidants,superoxide dismutase,glutathione peroxidase,catalase and malondialdehyde in the infected groups compared to the control group.While a decrease in the level of malondialdehyde and an increase in the levels of total antioxidants, superoxide dismutase, glutathione peroxidase and catalase in the treatment groups compared to the infected group were significantly show.Based on the results obtained,nanochitosan carrying bee pollen showed a modulating effect on the levels of antioxidants and lipid peroxidation in the liver tissue of ratinfected with bacteria.Therefore,it can probably be used as a promising therapeutic strategy
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