The Linkage Map of Carthamus tinctorius L. Based on RAPD, SRAP and EST-SSR Markers
محورهای موضوعی : Research On Crop EcophysiologyAhmad Reza Golparvar 1 , SHAKIBA IRAVANI 2 , Hamideh Zamanpour Shahmansouri 3 , Ghazaleh Halvaei 4 , Amin Hadipanah 5
1 - Department of Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Department of School of Health Science, Faculty of Dentistry, University of Georgia, Tbilisi, Georgia
3 - Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran
4 - Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
5 - Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran
کلید واژه: Keywords: Genetic map, Safflower, Accession, Molecular markers, Genetic control.,
چکیده مقاله :
The Linkage Map of Carthamus tinctorius L. Based on RAPD, SRAP and EST-SSR Markers AHMAD REZA GOLPARVAR1*, SHAKIBA IRAVANI2, HAMIDEH ZAMA POUR SHAHMANSOURI3, GHAZALEH HALVAEI4, AMIN HADIPANAH5 1-Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University ,Isfahan, Iran 2-Department of School of Health Science, Faculty of Dentistry, University of Georgia, Tbilisi, Georgia 3-Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran 4-Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran 5-Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran * Corresponding Author E-mail: dragolparvar@gmail.com Received: 10 October 2023 Accepted: 8 January 2024 ABSTRACT Carthamus tinctorius L. (2n = 2x = 24), commonly known as safflower, is widely cultivated. The genetic map of safflower (C. tinctorius) constructed using RAPD, SRAP and EST-SSR markers. An F2 population of 117 progenies derived from a cross between cultivated C. tinctorius L. and a wild accession from C. oxyacanthus species was used to generate the map during 2015-2016. A total of 101 markers, including 49 RAPD, 40 SRAP and 22 EST-SSR were identified and used for genetic linkage group analysis. The linkage map consisted of 9 major linkage groups. The map covered a total length of 1760.9 cM with an average marker density of 31.4 cM between two adjacent markers. In the safflower map, 56 markers (41 RAPD, 14 SRAP and 1 EST-SSR) were linked. Construction of linkage map is an important first step towards detecting genes controlling agronomically important traits and can show the location of genetic loci along the chromosomes.
The Linkage Map of Carthamus tinctorius L. Based on RAPD, SRAP and EST-SSR Markers AHMAD REZA GOLPARVAR1*, SHAKIBA IRAVANI2, HAMIDEH ZAMA POUR SHAHMANSOURI3, GHAZALEH HALVAEI4, AMIN HADIPANAH5 1-Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University ,Isfahan, Iran 2-Department of School of Health Science, Faculty of Dentistry, University of Georgia, Tbilisi, Georgia 3-Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran 4-Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran 5-Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran * Corresponding Author E-mail: dragolparvar@gmail.com Received: 10 October 2023 Accepted: 8 January 2024 ABSTRACT Carthamus tinctorius L. (2n = 2x = 24), commonly known as safflower, is widely cultivated. The genetic map of safflower (C. tinctorius) constructed using RAPD, SRAP and EST-SSR markers. An F2 population of 117 progenies derived from a cross between cultivated C. tinctorius L. and a wild accession from C. oxyacanthus species was used to generate the map during 2015-2016. A total of 101 markers, including 49 RAPD, 40 SRAP and 22 EST-SSR were identified and used for genetic linkage group analysis. The linkage map consisted of 9 major linkage groups. The map covered a total length of 1760.9 cM with an average marker density of 31.4 cM between two adjacent markers. In the safflower map, 56 markers (41 RAPD, 14 SRAP and 1 EST-SSR) were linked. Construction of linkage map is an important first step towards detecting genes controlling agronomically important traits and can show the location of genetic loci along the chromosomes.
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