The Linkage Map of Carthamus tinctorius L. Based on RAPD, SRAP and EST-SSR Markers
الموضوعات : Research On Crop EcophysiologyAhmad Reza Golparvar 1 , SHAKIBA IRAVANI 2 , Hamideh Zamanpour Shahmansouri 3 , Ghazaleh Halvaei 4 , Amin Hadipanah 5
1 - Department of Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Department of School of Health Science, Faculty of Dentistry, University of Georgia, Tbilisi, Georgia
3 - Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran
4 - Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
5 - Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran
الکلمات المفتاحية: Keywords: Genetic map, Safflower, Accession, Molecular markers, Genetic control.,
ملخص المقالة :
The Linkage Map of Carthamus tinctorius L. Based on RAPD, SRAP and EST-SSR Markers AHMAD REZA GOLPARVAR1*, SHAKIBA IRAVANI2, HAMIDEH ZAMA POUR SHAHMANSOURI3, GHAZALEH HALVAEI4, AMIN HADIPANAH5 1-Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University ,Isfahan, Iran 2-Department of School of Health Science, Faculty of Dentistry, University of Georgia, Tbilisi, Georgia 3-Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran 4-Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran 5-Department of Plant Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran * Corresponding Author E-mail: dragolparvar@gmail.com Received: 10 October 2023 Accepted: 8 January 2024 ABSTRACT Carthamus tinctorius L. (2n = 2x = 24), commonly known as safflower, is widely cultivated. The genetic map of safflower (C. tinctorius) constructed using RAPD, SRAP and EST-SSR markers. An F2 population of 117 progenies derived from a cross between cultivated C. tinctorius L. and a wild accession from C. oxyacanthus species was used to generate the map during 2015-2016. A total of 101 markers, including 49 RAPD, 40 SRAP and 22 EST-SSR were identified and used for genetic linkage group analysis. The linkage map consisted of 9 major linkage groups. The map covered a total length of 1760.9 cM with an average marker density of 31.4 cM between two adjacent markers. In the safflower map, 56 markers (41 RAPD, 14 SRAP and 1 EST-SSR) were linked. Construction of linkage map is an important first step towards detecting genes controlling agronomically important traits and can show the location of genetic loci along the chromosomes.
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