Effect of high intensity interval training with nanocurcumin on fertility and oxidative index in diabetic rats
محورهای موضوعی : فیزیولوژی
1 - Department of Physical Education, Faculty of Science, Urmia Branch, Islamic Azad University, Urmia, Iran
کلید واژه: Diabetes, High interval intensity training, Oxidative stress, Nanocurcumin, Male wistar rats,
چکیده مقاله :
This study examines the combined effects of high-intensity interval training and Nanocurcumin on sperm quality and oxidative stress parameters in diabetic rats. Forty male Wistar rats (two months old, 200 ± 5g) were randomly assigned to five groups: control, diabetic mellitus (DM), DM with Nanocurcumin, DM with high-intensity interval training, and DM with both Nanocurcumin and high-intensity interval training. Diabetes which received a high-fat and high fructose diet for 2 months followed by a single intraperitoneal injection of streptozotocin, and the Nanocurcumin and high-intensity interval training groups received their respective treatments for two months. Sperm count, motility, viability, DNA integrity, testosterone levels, and oxidative stress markers, including malondialdehyde (MDA), total antioxidant capacity (TAC), and catalase (CAT), were evaluated. Data were analyzed using SPSS version 18.0, applying a one-way ANOVA to assess group differences, followed by Tukey’s post-hoc test for pairwise comparisons (p < 0.05). Diabetic rats demonstrated a reduction in sperm count, motility, and viability, alongside increased sperm DNA damage, decreased testosterone levels, and elevated MDA levels. Both Nanocurcumin and high-intensity interval training improved these parameters. The combination of Nanocurcumin and high-intensity interval training led to the most significant improvements, including higher sperm count, motility, and viability, reduced DNA damage, increased testosterone levels, and enhanced total antioxidant capacity, along with decreased MDA levels. Overall, the combined intervention of high-intensity interval training and Nanocurcumin effectively improves sperm quality and mitigates oxidative stress in diabetic rats.
This study examines the combined effects of high-intensity interval training and Nanocurcumin on sperm quality and oxidative stress parameters in diabetic rats. Forty male Wistar rats (two months old, 200 ± 5g) were randomly assigned to five groups: control, diabetic mellitus (DM), DM with Nanocurcumin, DM with high-intensity interval training, and DM with both Nanocurcumin and high-intensity interval training. Diabetes which received a high-fat and high fructose diet for 2 months followed by a single intraperitoneal injection of streptozotocin, and the Nanocurcumin and high-intensity interval training groups received their respective treatments for two months. Sperm count, motility, viability, DNA integrity, testosterone levels, and oxidative stress markers, including malondialdehyde (MDA), total antioxidant capacity (TAC), and catalase (CAT), were evaluated. Data were analyzed using SPSS version 18.0, applying a one-way ANOVA to assess group differences, followed by Tukey’s post-hoc test for pairwise comparisons (p < 0.05). Diabetic rats demonstrated a reduction in sperm count, motility, and viability, alongside increased sperm DNA damage, decreased testosterone levels, and elevated MDA levels. Both Nanocurcumin and high-intensity interval training improved these parameters. The combination of Nanocurcumin and high-intensity interval training led to the most significant improvements, including higher sperm count, motility, and viability, reduced DNA damage, increased testosterone levels, and enhanced total antioxidant capacity, along with decreased MDA levels. Overall, the combined intervention of high-intensity interval training and Nanocurcumin effectively improves sperm quality and mitigates oxidative stress in diabetic rats.
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