تأثیر سالسیلیک اسید بر برخی خصوصیات فیزیولوژیکی گیاه گشنیز (Coriandurum sativum L.)
محورهای موضوعی : اکوفیزیولوژی و فیتوشیمی گیاهان دارویی و معطرصالحه نادری 1 , حمیده خواجه 2 , حسن احمدی 3
1 - دانشجوی دکتری اصلاح نباتات، دانشکده کشاورزی، دانشگاه زابل
2 - عضو باشگاه پژوهشگران جوان و نخبگان، گروه تربیت بدنی، دانشگاه آزاد اسلامی واحد زابل، ایران
3 - کارشناس پژوهشکده زیست فناوری، دانشگاه زابل، ایران
کلید واژه: گشنیز, آنزیم‌های آنتی اکسیدان, رنگیزه‌های فتوسنتزی و سالسیلیک اسید,
چکیده مقاله :
این پژوهش به منظور بررسی تأثیرسطوح مختلف سالسیلیک اسید بر میزان فعالیت آنزیم های آنتی اکسیدان پراکسیداز (POD) و گایاکول پراکسیداز (GPX) و پلی فنل اکسیداز (PPO)، کلروفیل b، a و کارتنوئید گیاه دارویی گشنیز، در قالب طرح کاملاُ تصادفی در سه تکرار در پژوهشکده زیست فناوری دانشگاه زابل در سال 1392 انجام شد. تیمار اسید سالسیلیک اسید در چهار سطح 0، 1، 5/1 و 2 میلی مولار بود. نتایج نشان داد در سطح 2 میلی مولار فعالیت آنزیم های آنتی اکسیدان پراکسیداز (POD) و گایاکول پراکسیداز (GPX) و پلی فنل اکسیداز (PPO)، کلروفیل b، a و کارتنوئید نسبت به شاهد به ترتیب 96/72، 22/90، 70/91، 34/75، 34/85 و 12/81 درصد افزایش معنی داری نشان داد. به نظر می رسد سالسیلیک اسید با تولید آنتی اکسیدان ها و افزایش رنگیزه های فتوسنتزی در غلظت های بالاتر از 1 میلی مولار تا حدودی باعث بهبود رشد در گیاه گشنیز در شرایط آزمایش گردد.
In order to study the effect of salicylic acid on antioxidant enzymes activity such as peroxidase (POD), gayacol peroxidase (GPX) and poly phenol oxidase (PPO), chlorophyll a, b and carotenoid in Coriander, this study was done as completely randomized whit three replication in Biocenter of Zabol University, 2014. salicylic acid was in four level (0, 1, 1.5 and 2 mM). In comparison with control, the result showed antioxidant enzymes activity such as peroxidase (POD), gayacol peroxidase (GPX) and poly phenol oxidase (PPO), chlorophyll a, b and carotenoid increased 72.96, 90.22, 91.70, 75.34, 85.34 and 81.12 percent with control in 2 mM. It seems that in concentration higher than 1 mM salicylic acid, with producing antioxidants and increasing photosynthesis pigments, improved partially growth in Coriander at experimental condition.
امیدبیگی، ر. 1379. رهیافتهای تولید و فرآوری گیاهان دارویی. طراحان نشر. جلد اول.283 ص.
امیدبیگی، ر. 1374. رهیافتهای تولید و فرآوری گیاهان دارویی. انتشارت فکر روز. جلد اول.183 ص.
زرگری، ع. 1369. گیاهان دارویی. جلد سوم. انتشارات دانشگاه تهران.923 ص.
عزتی، پ. 1381. بررسی تاثیر تراکم بر عملکرد و ماده موثره بادرنجبویه. پایاننامه کارشناسی ارشد زراعت، دانشگاه آزاد واحد ورامین، 120 ص.
Abdel-Wahed, M.S.A. Amin, A.A. Rashed, M. 2006. Physiological effect of some chemical constituents of yellow maize plants. World Journal of Agriculture Science. 2: 149-155.
Agata, I. Kusakabe, H. Hatano, T. and Nishi be, O.T. 1993. Melitric acids A and B, new trimeri c caffei c acid derivatives from Melissa officinalis. Chemical and Pharmaceutical Bulletin. 41: 1608-1611.
Alscher, R. G. Erturk, N. and Heath, L. S. 2002. Role of superoxide dismutase (SOD) in controlling oxidative stress in plants. Journal of Experimental Botany. 53: 1331-1341.
Bennett, C. 2003. Plant extract improves cognitive function in Alzheimer' s disease. Health-News. Co. UK. 172.PP.
Chakraborty, U. Tongden, C. 2005. Evaluation of heat acclimation and salicylic acid treatments as potent inducers of thermotolerance in Cicerarietinum L. Current Science. 89: 384-389.
Eraslan, F. Inal, A. David, J. and Gunes, A. 2008. Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activity in spinach (Spinaciaoleracea L.cv . Matado) grown under boron toxicity and salinity. Plant Growth Regulation. 55: 207-219.
Fielding, J. L. Hall, J. 1978. A biochemical and cytochemical study of peroxidase activity in root of pisum sativam. Journal of Experimental Botany. 29:981-989.
Foyer, C.H. and Halliwell, B. 1976. The presence of glutathione and glutathione reductasein chloroplast: a proposed role in ascorbic acid metabolism. Planta. 133: 21-25.
Ghani, N. Setia, R. C. Setia, N. 2002. Effects of paclobutrazol and salicylic acid on chlorophyll content, hill activity and yield components in Brassica napus L. (cv. GSL 1). Phytomorphology. 52, 83-87.
Guo, Z.Y. Xing, R. E. Liu,S. Yu, H.H. Wang, P. B. Li, C. P. and Li, P.C. 2005. The synthesis and antioxidant activity of the Schiff bases of chitosan and carboxy methyl chitosan.Bioorg. Med. Chemictry. 15:4,600-4,603.
Gutierrez-Coronado, M. Trejo, C.L. Larque-Saavedra, A. 1998. Effects of salicylic acid on the growth of roots and shoots in soybean. Plant Physiol0gy Biochemistry. 36: 563-565.
Hayat, S. Ahmad, A. 2007. Salicylic Acid: A Plant Hormone. Springer. 97-99.
Iturbe-ormaetxe, I. Escuredo, P.R. Arrese-Igor, C. and Becana, M. 1998. Oxidative damage in pea plant exposed to water deficit or paraquat. Journal of Plant Physiology. 116: 173-181.
Janda, T. Szalai, G. Tari, I. Paldi, E. 1999. Hydroponic treatment with salicylic acid decreases the effect of chilling injury in maize (Zea mays L.) plants. Planta. 208: 175-180
Jiang, Y. and Huang, N. 2001. Drought and heat stress injury to two cool -season turfgrasses in relati on to antioxidant metabolism and lipid peroxidati on. Crop science. 41: 436-442.
Khodary, S. E. A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed Maizeplant. International Journal of Biology. 6: 5-8.
Laspina, N.V. Groppa, M.D. Tomaro, M.L. and Benavides, M.P. 2005. Nitric oxide protects sunflower leaves against Cd-induced oxidative stress. Plant Science. 169: 323-330.
Malamy. J. Klessig, D.F. 1992. Salicylic acid and plant disease resistance. J of Plant. 2. 645-654.
Mckersie, D.B. and Leshem, Y. 1994. Stress and Coping in Cultivated Plants. Kluwer Acad. Pub, London.
Mikolajewicz, M. and Filoda, G. 1998. Septoria Melissa Desm Control on common bahu (Melissa officinalis L.) Herba, Poloinca. 44: 172-174
Munn,S. Alegre, L. 2000. The significance of beta carotene, alpha, tocopherol and the xanthophyll cycle in droughted Melissa officinalis plant. Journal of plant physiology. 27:139-146.
Noreen, Z. Ashraf, M.Akram, N. A. 2010. Salt-induced regulation of some key antioxidant enzymes and physiobiochemical phenomena in five diveres cultivars of turnip (Brassica rapa L.). Journal of Agronomy and Crop Science. 196: 273-285.
Pak Mehr, A. 2009. Priming effect of salicylic acid on some morphological and physiological properties of cowpea under water stress. MSc thesis, Faculty of Agriculture, Zanjan University. [In Persian with English Summery].
Raskin, I. 1992. Role of salicylic acid in plants.Ann. Rev. Plant Physiol. Plant Molcular Biology. 43: 439-463.
Raymond, M. Poulin, E. Boirox, V. Dupont, E. and Pasteur, N. 1993. Stability of insecticide resistance due to amplification of esterase genes in Cluexpipiens. Heredity. 70: 301-307.
Schulz, H. Jobert, M. Hubner, W. 1998. The quantitative EEG as a screening instrument to identify sedative effects of singl e doses of Plant extracts in comparison with diazepam. Phytomedicine. 5: 449-458.
Senaratna, T. Touchell, D. Bunn, E. Dixon, K. 2000. Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Reg. 30: 157-161.
Shabani, L. Ehsanpour, A.A. 2009. Induction of antioxidant enzymes, phenolic and flavonoid compounds in in vitro culture of licorice (Glycyrrhizaglabra L.) using methyl jasmonate and salicylic acid. Iranian Journal of Biology. 22: 691-703. [In Persian with English Summery].
Smirnoff, N. 1993. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytology. 125: 27-58.
Szepesi, A. Csiszar, I. Bajkan, S. Gemes, K. Horvath, F. Laszlo, E. Deer, A.K. Simon, M.L. Tari, I. 2005. Role of salicylic acid pretreatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biol. Szegediensis. 49: 123-125.
Urbanek, H. Kuzniak-Gebarowska, E. and Herka, K. 1991. Elicition of defense responses in bean leaves by Botrytis cinerea poly glacturonase. Journal of Physiology Plant. 13: 43-50.
Vangronsveld, J. and Clijsters, H. 1994. Toxic effects of metals. In: Plants and the Chemical Elements: Biochemistry, Uptake, Tolerance and Toxicity. Edited by M.E. Farago, Wienheim.
Wake, G. Court, J. Pickering, A. Lewis, R. Wilkins, R. and Perry, E. 2000. CNS acetylcholine receptor activity in European medicinal Plant straditionally used to improve failing memory. Journal of Ethno pharmacology. 69: 105-114.
Weizman, Z. Alkrisnawi , S. and Golldfarb, D. Bitran, C. 1993. Efficiency of herbal tea preparation in infantile colic. Journal of Pediatrics. 122: 650-652.
Werker, E.Putievsky, E. Ravid, U. Dudai, N. and Katzir, I. 1993. Glandular h and essential oil in developing leaves of Ocimum basilicum L. (Lamiaceae). Journal of Annual Botany. 71: 43–50.
Yalpani, N. Raskin, I. 1993. Salicylic acid: A systemic signal in plant disease resistance. Trend Microbiol. 1, 88-92.
Yanishlieva, N. and Marinova, E. 1998. Activity and mechanism of action of antioxidants in lipids. Recent Research Developments in oil. Chemistry. 2: 1-14.
Yu, L.J. Lan, W.Z. Qin, W.M. Xu, H.B. 2001. Effects of salicylic acid on fungal elicitor induced membrane-lipid peroxidation and taxol production in cell suspension cultures of Taxuschinensis. Process. Biochem. 37: 477-82.
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