Role of Extracellular Hydrolytic and Ligninolytic Enzymes as Virulence Factors of the Dematophora necatrix in the Activation of Basal Resistance of Walnut
Nima Khaledi
1
(
Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
)
Mahdi Rezaei
2
(
Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
)
Farshid Hassani
3
(
Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
)
کلید واژه: Basal resistance, Defence responses, Phenolics, Soil-borne, Virulence,
چکیده مقاله :
Dematophora necatrix is one of the most destructive soil-borne fungal pathogens causing white root rot disease on walnuts in Iran and worldwide. No walnut rootstock genotypes have shown complete resistance to this disease. This study aims to identify and monitor the prevalence of D. necatrix in traditional and commercial walnut orchards as well as to explore extracellular the hydrolytic and ligninolytic enzymes produced by the fungal isolates plus variations in virulence and aggressiveness in vivo. We also examined the role of biochemical mechanisms, including the activities of oxidative enzymes and defensive compounds involved in the basal resistance of walnuts to the necrotrophic D. necatrix. In this study, we investigated the presence of the causal agent of white rot disease in root and soil samples collected from commercial and traditional gardens in 17 regions of Iran. Then, we analysed the relationship between the activity of extracellular hydrolytic and ligninolytic enzymes produced by D. necatrix isolates together with variations in pathogenicity and aggressiveness in vivo. Next, the activity of guaiacol peroxidase (GPX), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), and level of defensive compounds were studied at various time points after inoculation by different D. necatrix isolates. All fungal isolates were identified as D. necatrix based on their morphological and molecular characteristics. Analysis of the activity of extracellular hydrolytic and ligninolytic enzymes produced by the fungal isolates revealed a higher activity of these enzymes for isolates presenting higher levels of virulence and aggressiveness in walnut seedlings. The current study suggests the critical role of GPX, PPO, and PAL in walnut resistance against D. necatrix, which was associated with lignification and phenolic production. These results provide novel information about the role of metabolism of phenolic compounds in basal resistance of walnut plant after inoculation by D. necatrix, where the level of defence response depends on the level of pathogenicity of isolates.
چکیده انگلیسی :
Dematophora necatrix is one of the most destructive soil-borne fungal pathogens causing white root rot disease on walnuts in Iran and worldwide. No walnut rootstock genotypes have shown complete resistance to this disease. This study aims to identify and monitor the prevalence of D. necatrix in traditional and commercial walnut orchards as well as to explore extracellular the hydrolytic and ligninolytic enzymes produced by the fungal isolates plus variations in virulence and aggressiveness in vivo. We also examined the role of biochemical mechanisms, including the activities of oxidative enzymes and defensive compounds involved in the basal resistance of walnuts to the necrotrophic D. necatrix. In this study, we investigated the presence of the causal agent of white rot disease in root and soil samples collected from commercial and traditional gardens in 17 regions of Iran. Then, we analysed the relationship between the activity of extracellular hydrolytic and ligninolytic enzymes produced by D. necatrix isolates together with variations in pathogenicity and aggressiveness in vivo. Next, the activity of guaiacol peroxidase (GPX), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), and level of defensive compounds were studied at various time points after inoculation by different D. necatrix isolates. All fungal isolates were identified as D. necatrix based on their morphological and molecular characteristics. Analysis of the activity of extracellular hydrolytic and ligninolytic enzymes produced by the fungal isolates revealed a higher activity of these enzymes for isolates presenting higher levels of virulence and aggressiveness in walnut seedlings. The current study suggests the critical role of GPX, PPO, and PAL in walnut resistance against D. necatrix, which was associated with lignification and phenolic production. These results provide novel information about the role of metabolism of phenolic compounds in basal resistance of walnut plant after inoculation by D. necatrix, where the level of defence response depends on the level of pathogenicity of isolates.
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