Investigating the Antimicrobial Effects of Encapsulated Nisin on the Growth and Binding Ability of Streptococcus mutans
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
1 - گروه میکروبیولوژی ، دانشکده علوم پایه ، واحد رشت ، دانشگاه آزاد اسلامی
کلید واژه: Microliposome, Nisin, growth inhibitory effects, Oral pathogens, Streptococcus mutans,
چکیده مقاله :
Nisin is an antimicrobial peptide consisting of 34 amino acid residues. Due to the presence of lanthionine
amino acids, it is classified as a lantibiotic and demonstrates effectiveness against numerous pathogenic microorganisms.
Although its effectiveness is greatly reduced in various clinical and environmental settings due
to its reaction with other compounds. New methods may be able to solve this problem in microcapsules and
control their release. The use in toothpaste helps control factors that contribute to tooth decay, one of the most
common diseases that humans face in their lifetime. The aim of this study was to investigate the effects of free
and encapsulated nisin on Streptococcus mutans and its growth kinetics. Nisin was encapsulated in lecithin and
alginate liposomes using the thin-layer hydration method. The size and distribution of nisin microliposomes
were determined using scanning electron microscopy and Dynamic Light Scattering (DLS) techniques. To
determine the minimum inhibitory concentration (MIC) of nisin microliposomes and free nisin on S. mutans
at various concentrations ranging from 0.78 to 25 μg/ml. To investigate the growth kinetics, the growth rate
or inhibition of S. mutans was studied by exposing it to concentrations equivalent to the MIC over a 50-hour
period at a wavelength of 600 nm. The study compared the effects of nisin and nisin microcapsules to those of
the antibiotics amoxicillin and penicillin. Microencapsulated nisin reduced the growth of planktonic strains of
S. mutans more effectively than free nisin. Nisin microliposomes at a concentration of 6.25 μg/ml inhibited the
growth of S. mutans, whereas the inhibitory effects of free nisin were observed at a concentration of 12.5 μg/ml
in both clinical and standard strains. Additionally, there was a significant reduction in the production of lactic
acid by S. mutans.Based on the current findings regarding the inhibitory effects and durability of nisin micro
encapsules, it is plausible that this compound could be utilized in combination with other methods to control
tooth decay and enhance the anti-caries efficacy.
Nisin is an antimicrobial peptide consisting of 34 amino acid residues. Due to the presence of lanthionine
amino acids, it is classified as a lantibiotic and demonstrates effectiveness against numerous pathogenic microorganisms.
Although its effectiveness is greatly reduced in various clinical and environmental settings due
to its reaction with other compounds. New methods may be able to solve this problem in microcapsules and
control their release. The use in toothpaste helps control factors that contribute to tooth decay, one of the most
common diseases that humans face in their lifetime. The aim of this study was to investigate the effects of free
and encapsulated nisin on Streptococcus mutans and its growth kinetics. Nisin was encapsulated in lecithin and
alginate liposomes using the thin-layer hydration method. The size and distribution of nisin microliposomes
were determined using scanning electron microscopy and Dynamic Light Scattering (DLS) techniques. To
determine the minimum inhibitory concentration (MIC) of nisin microliposomes and free nisin on S. mutans
at various concentrations ranging from 0.78 to 25 μg/ml. To investigate the growth kinetics, the growth rate
or inhibition of S. mutans was studied by exposing it to concentrations equivalent to the MIC over a 50-hour
period at a wavelength of 600 nm. The study compared the effects of nisin and nisin microcapsules to those of
the antibiotics amoxicillin and penicillin. Microencapsulated nisin reduced the growth of planktonic strains of
S. mutans more effectively than free nisin. Nisin microliposomes at a concentration of 6.25 μg/ml inhibited the
growth of S. mutans, whereas the inhibitory effects of free nisin were observed at a concentration of 12.5 μg/ml
in both clinical and standard strains. Additionally, there was a significant reduction in the production of lactic
acid by S. mutans.Based on the current findings regarding the inhibitory effects and durability of nisin micro
encapsules, it is plausible that this compound could be utilized in combination with other methods to control
tooth decay and enhance the anti-caries efficacy.
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