اثر مصرف جیره های مکمل شده با سطوح مختلف ویتامین E، سیزامین و تیموکوئینون بر چالش پاسخ سیستم ایمنی سلولی، ریخت شناسی روده، جمعیت میکروبی روده و بیان ژن MUC2 در بلدرچین های ژاپنی تخم‌گذار

محورهای موضوعی : فصلنامه زیست شناسی جانوری

یاسر رحیمیان ¹ (گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران)
فرشید خیری ² (گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران)
مصطفی فغانی ³ (گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران)

تاریخ دریافت : 1402/03/18 تاریخ پذیرش : 1402/05/05 تاریخ انتشار : 1402/12/01

کلید واژه: ویتامین E, سیزامین, تیموکوئینون, سیستم ایمنی سلولی, بیان ژن MUC2, بلدرچین ژاپنی تخم‌گذار,

چکیده مقاله :

به منظور بررسی اثر مصرف جیره های مکمل شده با سطوح مختلف ویتامین E، سیزامین و تیموکوئینون بر چالش پاسخ سیستم ایمنی سلولی ، ریخت شناسی، جمعیت میکروبی روده و بیان ژن MUC2 در بلدرچین های ژاپنی تخم‌گذار، تعداد210 قطعه بلدرچین ژاپنی ماده در سن 35-85 روزگی با 7 تیمار آزمایشی شامل گروه شاهد و مصرف کننده ویتامین E، تیموکوئنیون و سیزامین با سطوح 100 و 200 میلی‌گرم/کیلوگرم از خوراک استاندارد با 5 تکرار و 6 قطعه بلدرچین تخم‌گذار در هر تکرار استقاده گردید. در انتهای دوره (85 روزگی) پس از کشتار دو قطعه پرنده از هر تکرار وزن نسبی اندام های ایمنی (تیموس، طحال و بورس فابریسیوس) و فعالیت شاخص های کبدی نظیر آلبومین، آنزیم های آلکالین فسفاتاز (ALP)، آلانین ترانس آمیناز (ALT) و آسپارتات ترانس آمیناز (AST) بررسی شد. پاسخ های ایمنی سلولی نظیر تست چالش حساسیت به دی نیتروکلروبنزن و پاسخ به تزریق میتوژن فیتوهماگلوتینین به وب بال چپ مطالعه شد. برای ارزیابی مورفولوژی بافت روده و جمعیت فلور میکروبی روده باریک، نمونه برداری صورت گرفت. برای بررسی بیان ژن MUC2نمونه هایی از ژژنوم جمع آوری و از روش RT-PCR استفاده شد. نتایج نشان داد مصرف جیره های مکمل شده سبب افزایش وزن نسبی معنی دار بورس فابرسیوس (05/0 ≤ p) و کاهش غیرمعنی داری فعالیت آنزیم های کبدی گردید (05/0≥ p). افزایش معنی دار فلور میکروبی مفید (لاکتوباسیلوس ها) و در مقابل کاهش جمعیت کلونی باکتری های اشرشیاکولی و سالمونلا انتریکا در روده همراه با افزایش سطح ویلی ها و تراکم تعداد سلول‌های گابلت نشان دهنده اثرات مفید ترکیبات فعال استفاده شده در بهبود وضعیت سلامت بلدرچین ها بود (05/0 ≤ p). مصرف جیره های مکمل شده با سطوح ویتامین E، سیزامین و تیموکوئنیون سبب افزایش بیان mRNA ژن MUC2در روده شد. نتایج نشان دهنده ی سودمندی مصرف ویتامین E، سیزامین و تیموکوئنیون بر پاسخ سیستم ایمنی سلولی، مورفولوژی، فلور میکروبی روده و بیان mRNA ژن MUC2 در بلدرچین های ژاپنی تخم‌گذار بود.

چکیده انگلیسی:

In order to investigate the effect of consuming diets supplemented with different levels of vitamin E, sesamin and thymoquinone on cellular immune system challenges, intestinal morphology, intestinal microbial population and MUC2 gene expression in laying Japanese quails, 210 female Japanese quails aged 85-35 days with 7 experimental treatments including the control group and those consuming vitamin E, thymoquinone and sesamin at levels of 100 and 200 ml.gram/kg of standard feed was used with 5 repetitions and 6 pieces of laying quail in each repetition. At the end of the period (85 days), after killing two quails from each repetition, the relative weight of thymus, spleen and bursa of fabricius and the liver enzymes activity indices such as albumin, alkaline phosphatase (ALP), alanine transaminase (ALT) and aspartate transaminase (AST) were investigated. Dinitrochlorobenzene sensitization challenge test and response to the injection of mitogen phytohemagglutinin into the left wing web were studied. Sampling was done to evaluate the morphology of the intestinal tissue and the microbial flora population of the small intestine. To investigate the expression of MUC2 gene, samples from jejunum were collected and RT-PCR method was performed. The results showed that the consumption of supplemented diets caused a significant increase in the bursa.f relative weight (p ≤ 0.05) and a non-significant improvement of the liver enzyemes activity (p ≥ 0.05). The significant increase of beneficial microbial flora (Lactobacillus) and decrease of the colony population of (Escherichia coli and Salmonella enterica) bacteria in the intestine along with the increase in the surface of the villi and the density of the number of goblet cells indicated the beneficial effects of the active compounds used in improving the health status of quails (p ≤ 0.05). Consuming supplemented diets with levels of vitamin E, sesamin and thymoquinone caused an increase in MUC2 mRNA expression in the intestine. The results showed the benefits of vitamin E, sesamin and thymoquinone consumption on cell mediated mediated immunity, intestinal morphology, intestinal microbial flora and MUC2 mRNA expression in laying Japanese quails.

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