بررسی اثرات سایتوتوکسیک ناندرولون بر رده سلولی سرطان کولون (HCT) و ارزیابی فعالیت کاسپازهای 3، 8 و 99
محورهای موضوعی : فصلنامه زیست شناسی جانوری
زهرا اعلایی
1
(گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران)
عبدالحسین شیروی
2
(گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران)
رحیم احمدی
3
(گروه فیزیولوژی، واحد همدان، دانشگاه آزاد اسلامی، همدان، ایران)
ویدا حجتی
4
(گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران)
کلید واژه: زنده‌مانی, Hct, کاسپاز 3, کاسپاز 9, ناندرولون, کاسپاز 8,
چکیده مقاله :
سرطان کولون از مهمترین سرطان ها در سراسر دنیا محسوب می شود و سومین سرطان شایع در مردان و دومین سرطان شایع در زنان می باشد. مطالعات نشان داده اند که هورمون های استروئیدی جنسی بر عملکرد دستگاه گوارش و بر تکثیر سلول های سرطانی در سطح سلولی و مولکولی تاثیرگذارند. این مطالعه به بررسی اثرات ضد تکثیری ناندرولون بر رده سلولی سرطان کلون (HCT) و ارزیابی فعالیت کاسپازهای 3، 8 و 9 می پردازد. در این مطالعه آزمایشگاهی سلول های HCT به گروه شاهد (بدون تیمار) و گروه های تیمار شده با غلظت های625/0، 25/1، 5/2، 5 و 10 میلی گرم بر میلی‌لیتر ناندرولون تقسیم بندی شدند. اثر سیتوتوکسیک هورمون با استفاده از سنجش MTT اندازه گیری شد. میزان فعالیت کاسپازهای 3، 8 و 9 توسط کیت (Novex) مورد ارزیابی قرار گرفت. زنده مانی سلول های HCT در گروه دریافت کننده ی غلظت های 5/2، 5 و 10 میلی‌گرم بر میلی‌لیتر ناندرولون نسبت به گروه شاهد دچار کاهش معناداری شد (001/0p <) در حالی که فعالیت کاسپاز 3 و 9 (001/0p <) و 8 (01/0 < p) در رده سلولی HCT تیمار شده با ناندرولون، افزایش معناداری پیدا کرد. یافته های این تحقیق نشان دادند که ناندرولون دارای اثر سایتوتوکسیک بر روی سلول های سرطانی کولون می باشد و بخشی از این اثر حداقل از طریق افزایش فعالیت کاسپازهای 3 و 8 و 9 حاصل می شود.
The studies indicated that sex steroid hormones affect the digestive system function and proliferation of the cancer cells in the cell and molecular level. This study examines the anti-proliferative effects of Nandrolone on colon cancer (HCT) cell line and evaluates the activity of caspases of 3, 8, and 9. In this experimental study, HCT cells were divided into the control group (without treatment) and groups treated with 0.625, 1.25, 2.5, 5, and 10 mg/ml of Nandrolone. The cytotoxic effect of hormone was measured using MTT. The activity level of caspases 3, 8, and 9 were evaluated by the standard kit. The data were compared among the groups using one-way analysis of variance and t-test. HCT cell viability significantly decreased in the groups treated with 2.5, 5, and 10 mg/ml of Nandrolone compared to the control group (p < 0.001); However, caspase 3 and 9 (p < 0.001) and caspase 8 activity (p < 0.01) significantly increased. This study's findings showed that Nandrolone has a cytotoxic effect on colon cancer cells, and this effect is partly mediated by increasing the caspases of 3, 8, and 9 activities.
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