Improvement of the Microbial, Chemical, and Sensory Quality of Local Butter with Carum Copticum Essential Oil

محورهای موضوعی :

Fatemeh Ghalehnovi ¹ , Ayda Ghahremanian ² , Shaghayegh Mousavi ³ , Leila Manafi ⁴ , Ali Mehrabi ⁵ , Razzagh Mahmoudi. ⁶

1 - Department of Food Hygiene and Safety, School of Health, Qazvin University of Medical Sciences, Qazvin, Iran
2 - Department of Food Hygiene and Safety, School of Health, Qazvin University of Medical Sciences, Qazvin, Iran
3 - Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
4 - Health Products Safety Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
5 - Department of Food Hygiene and Safety, School of Health, Qazvin University of Medical Sciences, Qazvin, Iran
6 - Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

تاریخ دریافت : 1402/06/15 تاریخ پذیرش : 1402/09/04 تاریخ انتشار : 1403/10/30

کلید واژه: Essential oils, dairy products, Natural preservatives, GC-MASS, Ajwain,

چکیده مقاله :

This study was conducted to improve the microbial, chemical, and sensory quality of local butter offered in Qazvin City, Iran, with the Carum capticum essential oil (EO). Firstly, EO extraction was done by distillation method; its compounds were analyzed using GC-Mass. After determining the EO antimicrobial activity by disk diffusion and microdilution method, concentrations of 2 and 4% were selected for butter treatment. The samples were evaluated in terms of microbial (total bacterial count, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and yeast-mold), chemical (pH value and acidity), and sensory (color, flavor, odor, and overall acceptability), at days 1, 3, 5, 7, and 10. According to the results, thymol, p-cymene, and γ-terpinene were the main components of the EO. The minimum inhibitory concentration (MIC) ranged from 0.05 to 0.125 %; the lowest MIC was related to E. coli and the highest was related to Candida albicans. Microbial evaluation of treated butter showed that the colony counts of examined organisms on days 3, 5, 7, and 10 decreased significantly (P<0.05) in the presence of 4% EO. The concentration of 2% EO significantly (P<0.05) reduced the organisms' population except on the third day. In addition, the EO reduced the acidity and increased the pH value in different storage days (P<0.05). The sensory evaluation also showed more acceptability of EO-containing butter (P<0.05). Considering the effects of C. Copticum EO in improving butter quality and its abundance as a native plant in Iran, it seems a proper candidate for replacing chemical preservatives and producing new products.

چکیده انگلیسی:

This study was conducted to improve the microbial, chemical, and sensory quality of local butter offered in Qazvin City, Iran, with the Carum capticum essential oil (EO). Firstly, EO extraction was done by distillation method; its compounds were analyzed using GC-Mass. After determining the EO antimicrobial activity by disk diffusion and microdilution method, concentrations of 2 and 4% were selected for butter treatment. The samples were evaluated in terms of microbial (total bacterial count, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and yeast-mold), chemical (pH value and acidity), and sensory (color, flavor, odor, and overall acceptability), at days 1, 3, 5, 7, and 10. According to the results, thymol, p-cymene, and γ-terpinene were the main components of the EO. The minimum inhibitory concentration (MIC) ranged from 0.05 to 0.125 %; the lowest MIC was related to E. coli and the highest was related to Candida albicans. Microbial evaluation of treated butter showed that the colony counts of examined organisms on days 3, 5, 7, and 10 decreased significantly (P<0.05) in the presence of 4% EO. The concentration of 2% EO significantly (P<0.05) reduced the organisms' population except on the third day. In addition, the EO reduced the acidity and increased the pH value in different storage days (P<0.05). The sensory evaluation also showed more acceptability of EO-containing butter (P<0.05). Considering the effects of C. Copticum EO in improving butter quality and its abundance as a native plant in Iran, it seems a proper candidate for replacing chemical preservatives and producing new products.

منابع و مأخذ:

1. Allahghadri T., Rasooli I.., Owlia P., Nadooshan MJ., Ghazanfari T., Taghizadeh M., 2010. Antimicrobial property, antioxidant capacity, and cytotoxicity of essential oil from cumin produced in Iran. J Food Sci. 75(2), H54-H61.


2. Issa D., Najjar A., Greige-Gerges H., 2019. Nehme H. Screening of some essential oil constituents as potential inhibitors of the ATP synthase of Escherichia coli. J Food Sci. 84(1),138–46.


3. Hajimohammadi B., Eslami G., Manafi L., Athari SS., Boozhmehrani MJ., 2022. Sarcocystis sinensis in slaughtered cattle from Central of Iran. J Hell Vet Med Soc. 73(2), 4147–52.


4. Eslami G., Manafi L., Peletto S., 2016. DNA Extraction from Beef Harboring Sarcocystis spp.: Comparison of Three Different Analytical Methods. J food Qual hazards Control. 3(4), 128–33.


5. Manafi L., Aliakbarlu J., Dastmalchi Saei H., 2020. Antibiotic resistance and biofilm formation ability of Salmonella serotypes isolated from beef, mutton, and meat contact surfaces at retail. J Food Sci. 85(8), 2516–22.


6. Saatloo N.V., Ebrahiminejad B., Sadighara P., Manafi L., Yazdanfar N., Fallahizadeh S., 2023. Quantification and human health risk assessment of cadmium and lead in wheat flour on the Iranian market by atomic absorption spectrometry. Case Stud Chem Environ Eng. 8, 100438.


7. Meenambigai K,. Kokila R., Chandhirasekar K., Thendralmanikandan A., Kaliannan D., Ibrahim KS., et al. 2022. Green synthesis of selenium nanoparticles mediated by Nilgirianthus ciliates leaf extracts for antimicrobial activity on foodborne pathogenic microbes and pesticidal activity against Aedes aegypti with molecular docking. Biol Trace Elem Res. 200(6), 2948–62.


8. Manafi L., Aliakbarlu J., Dastmalchi Saei H., 2023. Susceptibility of Salmonella serotypes isolated from meat and meat contact surfaces to thermal, acidic, and alkaline treatments and disinfectants. Food Sci Nutr. 11(4), 1882-1890.


9. Mehdizadeh T., Hashemzadeh MS., Nazarizadeh A., Neyriz-Naghadehi M., Tat M., Ghalavand M., 2016. Chemical composition and antibacterial properties of Ocimum basilicum, Salvia officinalis and Trachyspermum ammi essential oils alone and in combination with nisin. Res J Pharmacogn. 3(4), 51–8.


10. Vidanagamage S.A., Pathiraje P., Perera O., 2016. Effects of Cinnamon (Cinnamomum verum) extract on functional properties of butter. Procedia food Sci. 6, 136–42.


11. Mahmoudi R., Khezri S., Ghajarbeygi P., Kaboudari A., Panahzadeh M., Mousavi SM., et al. 2019. Ziziphora clinopodioides essential oil effects on the physicochemical and microbial characteristics of cow milk butter during the storage at 4 C. Journal of Chemical Health Risks. 9(4), 303–9.


12. Mohammadi S., Aliakbarlu J., Tajik H., Manafi L., Mortazavi N., 2022. Inhibition of E. coli and bio-preservation of ground beef by Lactobacillus, black pepper extract and EDTA. Food Biosci. 101635.


13. Farhanghi A., Aliakbarlu J., Tajik H., Mortazavi N., Manafi L., Jalilzadeh-Amin G., 2022. Antibacterial interactions of pulegone and 1, 8-cineole with monolaurin ornisin against Staphylococcus aureus. Food Sci Nutr. 10(8), 2659-2666.


14. Yazdanfar N., Manafi L., Ebrahiminejad B., Mazaheri Y., Sadighara P., Basaran B., 2023. Evaluation of Sodium Benzoate and Potassium Sorbate Preservative Concentrations in Different Sauce Samples in Urmia, Iran. J Food Prot. 100118.


15. Pajohi Alamoti M., Bazargani-Gilani B., Mahmoudi R., Reale A., Pakbin B., Di Renzo T., et al. 2022. Essential oils from indigenous Iranian plants: a natural weapon vs. multidrug-resistant Escherichia coli. Microorganisms. 10(1), 109.


16. Mehdizadeh T., Tajik H., Jafarie S., Kaboudari A., 2019. Effect of Salvia officinalis L. extract on chemical, microbial, sensory and shelf life of rainbow trout fillet. Food Sci Biotechnol. 28, 1499–506.


17. Patil S.D., Maknikar P.P., Wankhade S.J., Ukesh C.S., Rai M.K., 2016. Chemical composition, antimicrobial and antioxidant activity ofessential oils from cumin and ajowan. Nusant Biosci. 8(1), 60-65


18. Mahmoudzadeh M., Hosseini H., Nasrollahzadeh J., Khaneghah A.M., Rismanchi M., Chaves R.D., 2016. Antibacterial activity of Carum copticum essential oil against Escherichia coli O157: H7 in meat: Stx genes expression. Curr Microbiol. 73(2), 265–72.


19. Asdagh A., Pirsa S., 2020. Bacterial and oxidative control of local butter with smart/active film based on pectin/nanoclay/Carum copticum essential oils/β-carotene. Int J Biol Macromol. 165, 156–68.


20. Mortazavi N., Aliakbarlu J., 2019. Antibacterial Effects of Ultrasound, Cinnamon Essential Oil, and Their Combination Against Listeria monocytogenes and Salmonella Typhimurium in Milk. J Food Sci. 84(12), 3700–6.


21. ISO EN., 2001. Milk and milk products--General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination. Czech standard institute, Prague, Czech Republic.


22. Marth E.H., 1978. Standard methods for the examination of dairy products. In: Standard methods for the examination of dairy products. pp. 416.


23. Moazeni M., Saharkhiz M.J., Hosseini A.A., 2012. In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. Vet Parasitol.187(1–2), 203–8.


24. Goudarzi G.R., Saharkhiz M.J., Sattari M., Zomorodian K., 2011. Antibacterial activity and chemical composition of Ajowan (Carum copticum Benth. & Hook) essential oil. J Agric Sci Technol. 13(2), 203–8.


25. Rabiey S., Hosseini H., Rezaei M., 2014. Use Carum copticum essential oil for controlling the Listeria monocytogenes growth in fish model system. Brazilian J Microbiol. 45, 89–96.


26. Sahaf B.Z., Moharramipour S., Meshkatalsadat M.H., 2007. Chemical constituents and fumigant toxicity of essential oil from Carum copticum against two stored product beetles. Insect Sci. 14(3), 213–8.


27. Mohagheghzadeh A., Faridi P., Ghasemi Y., 2007. Carum copticum Benth. & Hook., essential oil chemotypes. Food Chem. 100(3), 1217–9.


28. Kavoosi G., Tafsiry A., Ebdam A.A., Rowshan V., 2013. Evaluation of antioxidant and antimicrobial activities of essential oils from Carum copticum seed and Ferula assafoetida latex. J Food Sci. 78(2), T356--T361.


29. Khajeh M., Yamini Y., Sefidkon F., Bahramifar N., 2004. Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem. 86(4), 587–91.


30. Oliveira Ribeiro S., Fontaine V., Mathieu V., Zhiri A., Baudoux D., Stévigny C., 2020. Antibacterial and cytotoxic activities of ten commercially available essential oils. Antibiotics. 9(10), 717.


31. Zarshenas M.M., Samani S.M., Petramfar P., Moein M., 2014. Analysis of the essential oil components from different Carum copticum L. samples from Iran. Pharmacognosy Res. 6(1), 62.


32. Zhang Y., Liu X., Wang Y., Jiang P., Quek S., 2016. Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control. 59, 282–9.


33. Mirniyam G., Rahimmalek M., Arzani A., Matkowski A., Gharibi S., Szumny A., 2022. Changes in Essential Oil Composition, Polyphenolic Compounds and Antioxidant Capacity of Ajowan (Trachyspermum ammi L.) Populations in Response to Water Deficit. Foods. 11(19), 3084.


34. Mohammadi M., Masoumipour F., Hassanshahian M., Jafarinasab T., 2019. Study the antibacterial and antibiofilm activity of Carum copticum against antibiotic-resistant bacteria in planktonic and biofilm forms. Microb Pathog. 129, 99–105.


35. Mahmoudzadeh M., Hosseini H., Mahmoudzadeh L., Fard RMN., 2020. Comparative Effects of Carum copticum Essential Oil on Bacterial Growth and Shiga-Toxin Gene Expression of Escherichia coli O157: H7 at Abused Refrigerated Temperatures. Curr Microbiol. 77, 1660-1666.


36. Çakmakçi S., Gündougdu E., Daugdemir E., Erdougan Ü., 2014. Investigation of the possible use of black cumin (Nigella sativa L.) essential oil on butter stability. Kafkas Üniversitesi Vet Fakültesi Derg. 20(4), 533-539.


37. Thakaeng P., Wongsakul S., Yusoff MM., 2020. Development of value-added butter through the addition of green tea (Camellia sinensis L.) extract. Int Food Res J. 27(3), 465–74.


38. Dagdemir E., Cakmakci S., Gundogdu E., 2009. Effect of Thymus haussknechtii and Origanum acutidens essential oils on the stability of cow milk butter. Eur J Lipid Sci Technol. 111(11), 1118–23.


39. Zahedi H., Fadaei Noghani V., Khalafi L., 2015. Investigating physicochemical and sensory properties of yogurt enriched with oil and flavonoid extracted from orange peel. Res Innov Food Sci Technol. 4(1), 19–34.


40. Mahmoudi R., Zare P., Nosratpour S., 2015. Application of Teucrium polium essential oil and Lactobacillus casei in yoghurt. J Essent Oil Bear Plants. 18(2), 477–81.


41. Taherkhani P., Noori N., Akhondzadeh Basti A., Gandomi H., Alimohammadi M., 2015. Antimicrobial effects of kermanian black cumin (Bunium persicum Boiss.) essential oil in Gouda cheese matrix. J Med Plants. 14(54), 76–85.


42. Mahmoudi R., Zare P., Nosratpour S., 2019. Hygienic effects of Teucrium polium essential oil against Salmonella typhimorium LT2 in probiotic yoghurt. Studies in Medical Sciences. 25(8),769–777. [In Persian].