ELISA Evaluation of Erythromycin Residues in Honey Samples Collected from Different Areas of Qazvin, Iran

محورهای موضوعی :

Ali Mehrabi ¹ , Razzagh Mahmoudi ² , Nasim Biglari Khoshmaram ³ , Reza Norian ⁴ , Shaghayegh Mousavi ⁵ , Hatam Ebrahimi ⁶ , Ahad Alizadeh ⁷ , Maryam Kazemi ⁸

1 - Department of Food Hygiene and Safety, School of Public Health, Qazvin University of Medical sciences, Qazvin, Iran
2 - Professor, Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
3 - Department of Food Hygiene and Safety, School of Public Health, Qazvin University of Medical sciences, Qazvin, Iran
4 - Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5 - Ph.D Student in Molecular Medicine, Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
6 - Department of Food Hygiene and Safety, School of Public Health, Qazvin University of Medical sciences, Qazvin, Iran
7 - Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
8 - Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran

تاریخ دریافت : 1399/05/01 تاریخ پذیرش : 1400/10/25 تاریخ انتشار : 1402/09/10

کلید واژه: Honey, Elisa, Antibiotic residues, Erythromycin, Health hazards,

چکیده مقاله :

Nowadays, antibiotic residues as a global concern pose a threat not only to public health, but also to the food industry all around the world. There have been many studies on contamination sources of bee products, which the most important of them is evaluation of honey contamination extent with various antibiotics used in colonies. Antibiotic residues have a relatively long half life and may have direct toxic effects on consumers. Considering the adverse effects of this residues, we decided to measure the residual erythromycin in different samples of honey consumes in Qazvin, Iran. The present study is a cross-sectional study which conducted in different regions of Qazvin, Iran, in 2019. 80 samples of honey were collected from different regions in Qazvin. Using semi-quantitative ELISA, honey samples were evaluated for the presence of residual erythromycin. Based on the results obtained by ELISA, residual erythromycin was detected only in 8 samples (10.66%), of which, 5 samples (6.66%) and 3 samples (4%) had between 10-120 ng g-1 erythromycin and higher than 120 ng g-1 erythromycin (higher than detection limit of kit), respectively. According to the results, more than 96 percent of our honey samples lacked any erythromycin or had erythromycin concentration lower than allowable limit. The present study demonstrated that most of the honey samples collected from different regions of Qazvin lacked erythromycin residues, but due to the importance of drug residues on community health, annual screening of produced honey for the presence of drug residues by competent authorities.

منابع و مأخذ:

1. Ball D.W., 2007. The chemical composition of honey. Journal of chemical education. 84(10), 1643 -1646.


2. Libonatti C., Varela S., Basualdo M., 2014. Antibacterial activity of honey: A review of honey around the world. Journal of Microbiology and Antimicrobials. 6(3 (, 51-56.


3. Alvarez-Suarez JM., Gasparrini M., Forbes-Hernández T.Y., Mazzoni L., Giampieri F., 2014. The composition and biological activity of honey: a focus on Manuka honey. Journal of Foods. 3(3), 420-432.


4. El Hawari K., Al Iskandarani M., Mompelat S., Hurtaud‐Pessel D., Verdon E., 2017. Design for the transfer of a validated liquid chromatography/tandem mass spectrometry analytical method for the determination of antimicrobial residues in honey from low‐resolution to high‐resolution mass spectrometry. Journal of Rapid Communications in Mass Spectrometry. 31(13), 1103-1110.


5. Bueno-Costa F.M., Zambiazi R.C., Bohmer B.W., Chaves F.C., da Silva W.P., Zanusso J.T., Dutra I., 2016. Antibacterial and antioxidant activity of honeys from the state of Rio Grande do Sul, Brazil. Journal of LWT-Food Science and Technology. 65, 333-340.


6. Eteraf-Oskouei T., Najafi M., 2013. Traditional and modern uses of natural honey in human diseases: a review. Iranian Journal of Basic Medical Sciences. 16(6), 731-742.


7. Mahmoudi R., Norian R., Pajohi-Alamoti M., 2014. Antibiotic residues in Iranian honey by ELISA. International Journal of Food Properties. 17(10), 2367-2373.


8. Oniciuc E.A., Likotrafiti E., Alvarez-Molina A., Prieto M., López M., Alvarez-Ordóñez A., 2019. Food processing as a risk factor for antimicrobial resistance spread along the food chain. Journal of Current Opinion in Food Science. 30, 21-26.


9. Al-Waili N., Salom Kh., Al-Ghamdi A.,  Javed Ansari M., 2012.  Antibiotic, Pesticide, and Microbial Contaminants of Honey: Human Health Hazards. ScientificWorld Journal. 2012, 930849 -930858.


10. Wassenaa T.M., 2005. The use of antimicrobial agents in veterinary medicine and implications for human health. Crit Rev Microbiol. 31, 155-169.

11 .Mahmoudi R., Moosavy M., Norian R., Kazemi S., Asadi  M.R., Mardani K., 2014. Detection of oxytetracycline residues in honey samples using ELISA and HPLC methods. Journal of Pharmaceutical Sciences . 19(4), 145-150.

12 .Chen Y., Huang M., Wang Z., Chu J., Zhuang Y., Zhang S., 2013. Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis. Journal of  Bioprocess and biosystems engineering. 36(10), 1445-1453.

13 .El-Enshasy H., Mohamed N., Farid M., El-Diwany A., 2008. Improvement of erythromycin production by Saccharopolyspora erythraea in molasses based medium through cultivation medium optimization. Journal of Bioresource Technology. 99(10), 4263-4268.

14. 14. Bouzari S., Jafari A., Zarepoor M., 2007. Distribution of genes encoding toxins and antibiotic resistance patterns in diarrhoeagenic Escherichia coli isolates in Tehran. Journal of Eastern Mediterranean Health Journal. 13(2), 287-293.


15. Fazlara A., Izadi H., 2014. Survey on Tetracycline and Oxytetracycline antibiotic residues in honeys produced in some honey hives in Khuzestan province using HPLC method in 2012. Journal of Iranian Veterinary. 10(2), 65-73.


16. Mahmoudi R., Norian R., Gajarbeygi P., 2012. Survey of antibiotic residues in raw milk samples in Qazvin. Journal of Qazvin Univercity of  Medical Science. 18(1), 45-52.


17. Wang G., Zhang H.C., Liu J., Wang J.P., 2019. A receptor-based chemiluminescence enzyme linked immunosorbent assay for determination of tetracyclines in milk. Journal of Analytical Biochemistry. 564, 40-46.


18. Hosseinpour A., Ghajarbeygi P., Mahmoudi R., Norian R., Shahsavari S., 2020. Evaluation of Tetracycline Antibiotic Residue in Honey Samples using ELISA and HPLC . Journal of Chemical Health Risks. 10(0), 0-0.


19. Iranian Institute of Standards and Industrial Research. Honey - Residual Livestock Medicines - Sampling Method for Control - Test Method. National Standard of Iran .2003. 70-87.


20. Johnson S., Jadon N., 2010. Antibiotic residues in honey. Journal of Center for Science and Environment, India. 11(15), 20-22.


21. Thompson H.M., Waite R.J., Wilkins S., Brown M.A., Bigwood T., Shaw M., Ridgway C., Sharman M., 2005. Effects of European foulbrood treatment regime on oxytetracycline levels in honey extracted from treated honeybee (Apis mellifera) colonies and toxicity to brood. Journal of Food Additives and Contaminants, Part A. 22(6), 573 –578.


22. Bonvehi S.J., Gutierrez A.L., 2009. Residue of antibiotics and sulfanamides in honey from Basque country (NE spain). Journal of Food Agriculture. 85, 63-72.


23. Saridaki-Papakonstadinou M., Andredakis S., Burriel A., Tsachev I., 2006. Determination of tetracycline residues in Greek honey. Trakia Journal of Sciences. 4(1), 33-36.


24. Vidal T.L., Aguilera-Luiz Mdel M., Romero Gonzalez R., Frenich A.G., 2009. Multiclass analysis of antibiotic residues in honey by ultra-performance liquid chromatography tandem mass spectrometry. Journal of Agricultural and Food Chemistry. 57(5), 1760-1767.


25. Naumann G., Mahart E., Hinnelreich A., Mohring A., 2012. Traces of contaminationwell preserved in honey. Journal of Consumer Protection and Food Safety. 7(1), 35-43.


26. Gunes M.E., Gunes N., Cibik R., 2009. Oxytetracyclin and sulfanamide residue analysis of honey sample from southern Marmara region in Turkey. Bulgarian Journal of Agricultural Sciences. 15(2), 163-167.


27. Galarini R., Saluti G., Giusepponi D., Rossi R., 2015. Moretti S. Multiclass determination of 27 antibiotics in honey. Journal of Food Control. 48(1), 12-24.


28. Barrasso R., Bonerba E., Savarino A., Ceci E., Bozzo G., Tantillo G., 2019. Simultaneous Quantitative Detection of Six Families of Antibiotics in Honey Using A Biochip Multi-Array Technology. Journal of Veterinary Sciences. 6(1), 1-10.


29. Mahmoudi R., Golchin A., Farhoodi A., 2014. A Review on Antibiotic Residues in Animal-derived Foods in Iran over the Last Thirty Years. Journal of Mazandaran University of Medical Sciences. 24(119), 213-222.