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  • Study the Proliferation Rate of Human Bone Marrow Stem Cells Post Induced by Osteogenesis Differentiation Medium and Human Adipose Stem Cells Conditioned Medium
کد مقاله : JCHR-2301-1671 (R1) بازدید : 211 PDF XML

نوع مقاله: پژوهشی

Study the Proliferation Rate of Human Bone Marrow Stem Cells Post Induced by Osteogenesis Differentiation Medium and Human Adipose Stem Cells Conditioned Medium

Fateme Amirahmadi 1 ( Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran )
Maryam Haji Ghasem Kashani 2 ( Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran )
Meysam Nasiri 3 ( Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran )

تاریخ ارسال : 1401/11/01 تاریخ تایید : 1402/04/24

کلید واژه: Proliferation, Conditioned medium, Human stem cells, Osteogenic medium,

چکیده مقاله :

The human adipose stem cells (hADSCs) conditioned medium is easily prepared, transported, and stored. This medium is used in the treatment of diseases such as skin aging, wound, and scar repair, as well as nerve regeneration. In this research, the growth rate of human bone marrow stem cells (hBMSCs) under the effect of osteogenesis differentiation medium and the conditional medium was investigated separately. The third passage of hBMSCs was divided into three groups: 1- MED (cells cultured in α- MEM containing 10% fetal bovine serum), 2- OD (cells cultured in osteogenesis differentiation medium containing 7-10 M Dexamethason, 10 mM Beta-Glycerol-Phosphate, 50 ug ml-1 Ascorbic Acid bi-Phosphate, 10% FBS, 100 unit/ml Penicillin), and 3- CM (cells cultured in hADSCs conditioned medium). After 7 and 14 days, the proliferation rate was evaluated using the MTT method. The results showed that after 7 and 14 days of treatment, the rate of proliferation has increased considerably in both OD and CM groups versus the MED group, and the CM group versus the OD group. In addition, there has been an important increase in proliferation rate in both OD and CM groups compared to MED and in CM group compared to OD, 7 and 14 days post- treatment. Conditioned medium treatment effectively increased cell proliferation in hBMSCs, compared to osteogenic differentiation medium. This cell culture method provides the expansion of stem cells suitable for regenerative medicine.

چکیده انگلیسی :

The human adipose stem cells (hADSCs) conditioned medium is easily prepared, transported, and stored. This medium is used in the treatment of diseases such as skin aging, wound, and scar repair, as well as nerve regeneration. In this research, the growth rate of human bone marrow stem cells (hBMSCs) under the effect of osteogenesis differentiation medium and the conditional medium was investigated separately. The third passage of hBMSCs was divided into three groups: 1- MED (cells cultured in α- MEM containing 10% fetal bovine serum), 2- OD (cells cultured in osteogenesis differentiation medium containing 7-10 M Dexamethason, 10 mM Beta-Glycerol-Phosphate, 50 ug ml-1 Ascorbic Acid bi-Phosphate, 10% FBS, 100 unit/ml Penicillin), and 3- CM (cells cultured in hADSCs conditioned medium). After 7 and 14 days, the proliferation rate was evaluated using the MTT method. The results showed that after 7 and 14 days of treatment, the rate of proliferation has increased considerably in both OD and CM groups versus the MED group, and the CM group versus the OD group. In addition, there has been an important increase in proliferation rate in both OD and CM groups compared to MED and in CM group compared to OD, 7 and 14 days post- treatment. Conditioned medium treatment effectively increased cell proliferation in hBMSCs, compared to osteogenic differentiation medium. This cell culture method provides the expansion of stem cells suitable for regenerative medicine.

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