• الصفحة الرئيسية
  • Study the Proliferation Rate of Human Bone Marrow Stem Cells Post Induced by Osteogenesis Differentiation Medium and Human Adipose Stem Cells Conditioned Medium
رقم المقالة : JCHR-2301-1671 (R1) زيارة : 215 PDF XML

نوع المخطوط: ابحاث

Study the Proliferation Rate of Human Bone Marrow Stem Cells Post Induced by Osteogenesis Differentiation Medium and Human Adipose Stem Cells Conditioned Medium

Fateme Amirahmadi 1 ( Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran )
Maryam Haji Ghasem Kashani 2 ( Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran )
Meysam Nasiri 3 ( Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran )

تاريخ الإرسال : 28 السبت , جمادى الثانية, 1444 تاريخ التأكيد : 27 السبت , ذو الحجة, 1444

الکلمات المفتاحية: Proliferation, Conditioned medium, Human stem cells, Osteogenic medium,

ملخص المقالة :

The human adipose stem cells (hADSCs) conditioned medium is easily prepared, transported, and stored. This medium is used in the treatment of diseases such as skin aging, wound, and scar repair, as well as nerve regeneration. In this research, the growth rate of human bone marrow stem cells (hBMSCs) under the effect of osteogenesis differentiation medium and the conditional medium was investigated separately. The third passage of hBMSCs was divided into three groups: 1- MED (cells cultured in α- MEM containing 10% fetal bovine serum), 2- OD (cells cultured in osteogenesis differentiation medium containing 7-10 M Dexamethason, 10 mM Beta-Glycerol-Phosphate, 50 ug ml-1 Ascorbic Acid bi-Phosphate, 10% FBS, 100 unit/ml Penicillin), and 3- CM (cells cultured in hADSCs conditioned medium). After 7 and 14 days, the proliferation rate was evaluated using the MTT method. The results showed that after 7 and 14 days of treatment, the rate of proliferation has increased considerably in both OD and CM groups versus the MED group, and the CM group versus the OD group. In addition, there has been an important increase in proliferation rate in both OD and CM groups compared to MED and in CM group compared to OD, 7 and 14 days post- treatment. Conditioned medium treatment effectively increased cell proliferation in hBMSCs, compared to osteogenic differentiation medium. This cell culture method provides the expansion of stem cells suitable for regenerative medicine.

المصادر:


  1. Yu C., Chen P., Xu J., Liu Y., Li H., Wang L., 2020. hADSCs derived extracellular vesicles inhibit NLRP3inflammasome activation and dry eye. Scientific Reports. 10(1), 1-12.
    2.
  2. Fujisawa K., Hara K., Takami T., Okada S., Matsumoto T., Yamamoto N., 2018. Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells. Stem cell research & therapy. 9(1), 1-12.
    3.
  3. Pittenger M.F., Discher D.E., Péault B.M., Phinney D.G., Hare J.M., Caplan A.I., 2019. Mesenchymal stem cell perspective: cell biology to clinical progress. NPJ Regenerative Medicine. 4(1), 1-15.
    4.
  4. Matsubara T., Suardita K., Ishii M., Sugiyama M., Igarashi A., Oda R., 2005. Alveolar bone marrow as a cell source for regenerative medicine: differences between alveolar and iliac bone marrow stromal cells. Journal of Bone and Mineral Research. 20(3), 399-409.
    5.
  5. Dasari V.R., Veeravalli K.K., Dinh D.H., 2014. Mesenchymal stem cells in the treatment of spinal cord injuries: A review. World J Stem Cells. 6(2), 120-33.
    6.
  6. Mazini L., Rochette L., Amine M., Malka G., 2019. Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs). Int J Mol Sci. 20(10), 2523.
    7.
  7. Schweizer R., Tsuji W., Gorantla V.S., Marra K.G., Rubin J.P., Plock J.A., 2015. The role of adipose-derived stem cells in breast cancer progression and metastasis. Stem Cells Int. 120949.
    8.
  8. Pawitan J.A., 2014. Prospect of stem cell conditioned medium in regenerative medicine. Biomed Res Int. 965849.
    9.
  9. Osugi M., Katagiri W., Yoshimi R., Inukai T., Hibi H., Ueda M., 2012. Conditioned media from mesenchymal stem cells enhanced bone regeneration in rat calvarial bone defects. Tissue Eng Part A. 18(13-14), 1479-89.
    10.
  10. Azhdari Tafti Z., Mahmoodi M., Hajizadeh M.R., Ezzatizadeh V., Baharvand H., Vosough M., 2018. Conditioned Media Derived from Human Adipose Tissue Mesenchymal Stromal Cells Improves Primary Hepatocyte Maintenance. Cell J. 20(3), 377-87.
    11.
  11. Park B.S., Kim W.S., Choi J.S., Kim H.K., Won J.H., Ohkubo F., 2010. Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion. Biomedical Research. 31(1), 27-34.
    12.
  12. Okamoto T., Aoyama T., Nakayama T., Nakamata T., Hosaka T., Nishijo K., 2002. Clonal heterogeneity in differentiation potential of immortalized human mesenchymal stem cells. Biochemical and Biophysical Research Communications. 295(2), 354-61.
    13.
  13. Chen B., Yu J., Wang Q., Zhao Y., Sun L., Xu C., 2018. Human bone marrow mesenchymal stem cells promote gastric cancer growth via regulating c-Myc. Stem cells International. doi: 10.1155/2018/9501747
    14.
  14. Mohammed E.E., El-Zawahry M., Farrag AR.H., Aziz N.N.A., Sharaf-ElDin W., Abu-Shahba N., 2019. Osteogenic differentiation potential of human bone marrow and amniotic fluid-derived mesenchymal stem cells in vitro & in vivo. Open Access Macedonian Journal of Medical Sciences. 7(4), 507.
    15.
  15. Liu G.Y., Liu J., Wang Y.L., Liu Y., Shao Y., Han Y., 2016. Adipose‐derived mesenchymal stem cells ameliorate lipid metabolic disturbance in mice. Stem Cells Translational Medicine. 5(9), 1162-70.
    16.
  16. Tabatabai T.S., Kashani M.HG., Maskani R., Nasiri M., Amri S.A.N., Atashi A., 2021. Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. In Vitro Cellular & Developmental Biology-Animal. 1-9.
    17.
  17. Menezes K., Rosa B.G., Freitas C., da Cruz A.S., de Siqueira Santos R., Nascimento M.A., 2020. Human mesenchymal stromal/stem cells recruit resident pericytes and induce blood vessels maturation to repair experimental spinal cord injury in rats. Scientific Reports. 10(1), 1-21.
    18.
  18. Nakhaeifard M., Kashani M.H.G., Goudarzi I., Rezaei A., 2018. Conditioned medium protects dopaminergic neurons in parkinsonian rats. Cell Journal (Yakhteh). 20(3), 348.
    19.
  19. Pashaei A.sl R., Pashaiasl M., Ebrahimie E., Ataei M.L., Paknejad M., 2023. Apoptotic effects of human amniotic fluid mesenchymal stem cells conditioned medium on human MCF-7 breast cancer cell line. BioImpacts. 13(3), 191-206.
    20.
  20. Lee J.H., Park C.H., Chun K.H., Hong S.S., 2015. Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells. Oncology letters. 10(2), 730-6.
    21.
  21. Sanchooli T., Norouzian M., Teimouri M., Ardeshirylajimi A., Piryaei A., 2020. Adipose-derived stem cells conditioned media promote in vitro osteogenic differentiation of hypothyroid mesenchymal stem cells. Gene, Cell and Tissue. 7(3), e102267https://doi.org/10.5812/gct.102267
    22.
  22. Zhu M., Liu Y., Qin H., Tong S., Sun Q., Wang T., 2021. Osteogenically-induced exosomes stimulate osteogenesis of human adipose-derived stem cells. Cell and Tissue Banking. 22, 77-91.
    23.
  23. Yang X., Ma X., Don O., Song Y., Chen X., Liu J., 2020. Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/β-catenin. Stem Cell Research & Therapy. 11, 1-16.
    24.
  24. Maziarz A., Kocan B., Bester M., Budzik S., Cholewa M., Ochiya T., 2016. How electromagnetic fields can influence adult stem cells: positive and negative impacts. Stem Cell Res Ther. 7(1), 54.
    25.
  25. Leone L., Podda M.V., Grassi C., 2015. Impact of electromagnetic fields on stem cells: common mechanisms at the crossroad between adult neurogenesis and osteogenesis. Front Cell Neurosci. 9, 228.
    26.
  26. Noverina R., Widowati W., Ayuningtyas W., Kurniawan D., Afifah E., Laksmitawati D.R., 2019. Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs). Clinical Nutrition Experimental. 24, 34-44.
    27.
  27. Gharibi B., Hughes F.J., 2012. Effects of medium supplements on proliferation, differentiation potential, and in vitro expansion of mesenchymal stem cells. Stem Cells Translational Medicine. 1(11), 771-82.
    28.
  28. Choi K.M., Seo Y.K., Yoon H.H., Song K.Y., Kwon S.Y., Lee H.S., 2008. Effect of ascorbic acid on bone marrow-derived mesenchymal stem cell proliferation and differentiation. Journal of Bioscience and Bioengineering. 105(6), 586-94.
    29.
  29. Bellows C., Heersche J., Aubin J., 1990. Determination of the capacity for proliferation and differentiation of osteoprogenitor cells in the presence and absence of dexamethasone. Developmental Biology. 140(1), 132-8.
    30.
  30. Sanchooli T., Norouzian M., Teimouri M., Ardeshirylajimi A., Piryaei A., 2020. Adipose-derived stem cells conditioned media promote in vitro osteogenic differentiation of hypothyroid mesenchymal stem cells. Gene, Cell and Tissu. 7(3), 8-1.
    31.
  31. Ma Y., Kakudo N., Morimoto N., Lai F., Taketani S., Kusumoto K., 2019. Fibroblast growth factor-2 stimulates proliferation of human adipose-derived stem cells via Src activation. Stem Cell Research & Therapy. 10(1), 1-9.
    32.
  32. Zaragosi L.E., Ailhaud G., Dani C., 2006. Autocrine fibroblast growth factor 2 signaling is critical for self‐renewal of human multipotent adipose‐derived stem cells. Stem Cells. 24(11), 2412-9.
    33.
  33. Pizzute T., Li J., Zhang Y., Davis M.E., Pei M., 2016. Fibroblast growth factor ligand dependent proliferation and chondrogenic differentiation of synovium-derived stem cells and concomitant adaptation of Wnt/mitogen-activated protein kinase signals. Tissue Engineering Part A. 22(15-16), 1036-46.
    34.
  34. Hebert T.L., Wu X., Yu G., Goh B.C., Halvorsen Y.DC., Wang Z., 2009. Culture effects of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on cryopreserved human adipose‐derived stromal/stem cell proliferation and adipogenesis. Journal of Tissue Engineering and Regenerative Medicine. 3(7), 553-61.
    35.
  35. Chen J., Li Y., Hao H., Li C., Du Y., Hu Y., 2015. Mesenchymal stem cell conditioned medium promotes proliferation and migration of alveolar epithelial cells under septic conditions in vitro via the JNK-P38 signaling pathway. Cellular Physiology and Biochemistry. 37(5), 1830-46.
    36.

سند

Sanad is a platform for managing Azad University publications

الروابط

المراكز ذات الصلة

دعامة

الصفحات الرسمية

حقوق هذا الموقع الإلكتروني مملوكة لنظام SANAD Press Management. © 1442-1446

الصفحة الرئيسية| دخول| معلومات عنا| اتصل بنا|
[English] [فارسی] [en] [fa]