A Change in Leaves Protein Pattern of Some Pistachio Cultivars under Salinity Condition
محورهای موضوعی : MicrobiologyN Sohrabi 1 , A Tajabadipour 2 , N Motamed 3 , M Seyedi 4
1 - Iran's Pistachio Research Institute, P.O.Box; 77175-435, Rafasanjan-Iran
2 - Iran's Pistachio Research Institute, P.O.Box; 77175-435, Rafasanjan-Iran
3 - Department of biology, Faculty of science, University of Tehran
4 - National institutes of genetic engineering and biotechnology
کلید واژه: Pistachio, Salt stress, Protein profile, Protein extraction,
چکیده مقاله :
Plants are often subjected to several environmental stresses throughout their life cycle. Adaptation of plants to saline conditions may be due to some salt-related changes in the pattern of gene(s) expression. The physiological role of stress induced proteins is not yet clearly understood. However, it is believed that these proteins allow plants to produce biochemical and structural adjustments which enable them to adapt with the stress conditions. In this research we compared protein profile of 4 female cultivars and 2 male genotypes of Rafsanjan Pistachio in salt stress situation. The treatments were irrigated with saline water (EC = 5.5 dSm-1 ), without drainer, until appearing leaf phytotoxicity. Before and after saline stress leave proteins were extracted by using Damerval method, with some modification. The amount of total proteins was determined according the method of Bradford assay by used of BSA as standard solution. After uniformity of protein concentration, they were loaded on SDS-PAGE, in 12.5% separating gel and 5% stacking gel according to the method of Hames and Rickwood,. The results revealed that salinity induced changes in protein pattern and decreasing of total proteins in leaves of Pistachio. Some bands were decreased, increased, appeared or disappeared after salinity. Additionally a 40 KDa (kilo Dalton) band was increased in all samples. Moreover, this investigation reported the molecular weights of some salt responsive proteins.
