Effects of Different Fungicides on Paecilomyces formosus, the Causal Agent of Dieback and Canker Diseases of Pistachio

محورهای موضوعی : Plant Pathology

Mohammad Roushanpour ¹ , Maryam Soleimani ² , Mohammad Moradi ³ , Seyed Reza Fani ⁴ , Zohreh Jafari ⁵ , Ehsan Hosseinzadeh ⁶ , Claudia Probst ⁷

1 - Union of Rural Production Cooperative Companies of Zarand, Zarand, Iran
2 - Union of Rural Production Cooperative Companies of Zarand, Zarand, Iran
3 - Associated Professor, Pistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran
4 - Assistant Professor, Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran
5 - Union of Rural Production Cooperative Companies of Zarand, Zarand, Iran
6 - Union of Rural Production Cooperative Companies of Zarand, Zarand, Iran
7 - University of Applied Sciences, School of Engineering, Department of Agricultural Technology and Management, Wels, Austria

تاریخ دریافت : 1402/08/15 تاریخ پذیرش : 1403/03/26 تاریخ انتشار : 1404/02/17

کلید واژه: Chemical control, Complex disease, Decline, Fungicide trial, Pistachio,

چکیده مقاله :

In this study, the effect of 13 fungicides on mycelial growth, spore germination and sporulation of Paecilomyces formosus, the main causal agent of dieback disease, were investigated in vitro and in vivo. Treatments included Profiler^®, Rovral-TS^®, Elit^®, Oxychromes^®, Cidley Top^®, Cuprosit C^®, Ortivatop^®, Captan^®, Acrobat MZ^®, Benomyl^®, Luna^®, Folicur^® and Falcon^® at different concentrations of 500-3000 ppm. The results showed that the efficiency of different fungicides on fungal development, measured as mycelial growth, spore germination ranged from 36.8 to 100% and 21.7 to 100%, respectively. The inhibition of sporulation was between 0 to 4.3×10⁶ spores mL^-1. In vitro, the highest reduction in mycelium growth and spore germination was observed for the fungicides Benomyl^®, Luna^®, Folicur^®, and Falcon^® at concentrations of 1000, 500, 1500, 2000 and Ortivatop^®, Elite^®, Falcon^®, Benomyl^®, Captan^®, and Acrobat MZ^® at concentrations of 750, 2500, 2000 1000, 3000, 3000 ppm, respectively. In vivo, the effect of selected fungicides was evaluated on inoculated shoots. The rate of inhibition of pathogen progression using immersion inoculation and the vertical method was 18.7-43.2% and 39.8-45.5%, respectively. The highest inhibition (45.5%) was observed with Luna® fungicide at 500 ppm using the vertical method and the lowest inhibition (18.7%) was related to Benomyl fungicide at 1000 ppm using the immersion method (P≤0.01). No significant advantages were observed in fungicides applications in terms of DBP control compared to those control trees with no-spraying fungicides. In contrast, pruning showed comparative advantages in the management of DBP.

چکیده انگلیسی:

In this study, the effect of 13 fungicides on mycelial growth, spore germination and sporulation of Paecilomyces formosus, the main causal agent of dieback disease, were investigated in vitro and in vivo. Treatments included Profiler^®, Rovral-TS^®, Elit^®, Oxychromes^®, Cidley Top^®, Cuprosit C^®, Ortivatop^®, Captan^®, Acrobat MZ^®, Benomyl^®, Luna^®, Folicur^® and Falcon^® at different concentrations of 500-3000 ppm. The results showed that the efficiency of different fungicides on fungal development, measured as mycelial growth, spore germination ranged from 36.8 to 100% and 21.7 to 100%, respectively. The inhibition of sporulation was between 0 to 4.3×10⁶ spores mL^-1. In vitro, the highest reduction in mycelium growth and spore germination was observed for the fungicides Benomyl^®, Luna^®, Folicur^®, and Falcon^® at concentrations of 1000, 500, 1500, 2000 and Ortivatop^®, Elite^®, Falcon^®, Benomyl^®, Captan^®, and Acrobat MZ^® at concentrations of 750, 2500, 2000 1000, 3000, 3000 ppm, respectively. In vivo, the effect of selected fungicides was evaluated on inoculated shoots. The rate of inhibition of pathogen progression using immersion inoculation and the vertical method was 18.7-43.2% and 39.8-45.5%, respectively. The highest inhibition (45.5%) was observed with Luna® fungicide at 500 ppm using the vertical method and the lowest inhibition (18.7%) was related to Benomyl fungicide at 1000 ppm using the immersion method (P≤0.01). No significant advantages were observed in fungicides applications in terms of DBP control compared to those control trees with no-spraying fungicides. In contrast, pruning showed comparative advantages in the management of DBP.

منابع و مأخذ:

Abrishami MH (1994) Iranian Pistachio: Historical Knowledge. Iranian University Publication, Tehran.
Agrios G (2005) Plant Pathology. 5th Edition, Elsevier Academic Press, Amsterdam. 26-27,398-401.
Alipour H (2018) Photosynthesis properties and ion homeostasis of different pistachio cultivar seedlings in response to salinity stress. International Journal of Horticultural Science and Technology. 5(1), 19-29.
Alizadeh A, Alaei H, Ershad D (2000) Etiological study on dieback disease of pistachio trees in Rafsanjan. Journal of Agriculture Science. 1(2), 53-63.
Aminaei MM, Ershad D (1989) Pistachio dieback disease in Kerman province. In: Proceedings of 9th Iranian Plant Protection Congress, Mashhad, Iran. pp. 82.
Anonymous (2009) Folicur® 3.6 F Foliar Fungicide. https://www3.epa.gov/pesticides/- chem_search/ppls/000264-00752-20090617.pdf.
Anonymous (2021) FRAC Code List, 2021. Fungal control agents sorted by cross resistance pattern and mode of action. Syngenta Crop Protection AG Research and Development, Basel, Switzerland. https:// www.frac. info/docs/default-source/publications/frac-code-list/frac-code-list-2021--final.pdf?sfvrsn=f7ec499a_2.
Anonymous (2017a) Falcon® EC460 12X1L BOT BG. https://www.cropscience.bayer.rs/~/- media/Bayer%20CropScience/SWSlavic/Country-Serbia-Internet/ Bezbedonosni %20listovi /- 2018/ Falcon%20EC% 202018.pdf
Anonymous (2017b) Luna® Sensation. https://www3.epa.gov/pesticides/chem_search/- ppls/000264-01090-20170324.pdf.
Ashkan SM, Abusaidi D, Ershad D (1997) Etiological study of dieback and canker of Pistachio nut tree in Rafsanjan. Iran Journal of Plant Pathology. 20(1), 15-26.
Ashworth LJ, Gaona JR, Surber SA (1985) Nutritional diseases of pistachio trees, potassium and phosphorus deficiencies and chloride and boron toxicities. Phythopathlogy. 75, 1084-1091.
Baradaran GR, Ghasemi A (2010) Introducing Bacillus licheniformis as the causal agent of pistachio dieback in Iran. Archives of Phytopathology and Plant Protection. 43(6), 597-601. https://doi.org/10.1080/03235400801972368
Behzadi Rad P, Roozban MR, Karimi S, Ghahremani R, Vahdati K (2021) Osmolyte accumulation and sodium compartmentation has a key role in salinity tolerance of pistachios rootstocks. Agriculture. 11(8), 708.
Corazza L, Chilosi G, Avanzato D (1986) Dieback of pistachio branches caused by Botryosphaeria ribis. Annali dell'Istituto Sperimentale per la Patologia Vegetale. 11, 95-98.
Davarynejad GH, Azizi M, Akheratee M (2009) Effect of foliar nutrition on quality, quantity and of alternate bearing of Pistachio (Pistacia vera L.). Journal of Horticultural Science. 23(2), 1-10.
Dhingra OD, Sinclair JB (1995) Basic Plant Pathology Methods, 2nd ed. CRC press, London.
Edwards M, Taylor C, Ferguson L, Kester D (1998) Dieback and canker in Australian pistachios.Acta Hort. 470:596–603. https://doi.org/10.17660/ActaHortic.1998.470.84
Ershad D (2009) Fungi of Iran. Iranian Research Institute of Plant Protection publication, Tehran.
Facelli E, Taylor C, Scott E, Fegan M, Huys G, Noble RD, Swings J, Sedgley M (2005) Identification of the causal agent of pistachio dieback in Australia. European Journal of Plant Patholology. 112(2), 155-65. https://doi.org/10.1007/s10658-005-3120-9
FAO (2020) Pistachio production in 2021, Crops/Regions/World list/Production Quantity (pick lists). UN Food and Agriculture Organization, Corporate Statistical Database (FAOSTAT). Available: https://www.fao.org/faostat/en/#data/QCL. 2021. [Accessed December 27 2023].
Ghelichi M, Mohammadi A, Haghdel M, Eskandari A (2012) Distribution of pistachio dieback in Khorasan-Razavi province and application of some fungicides for the disease control. Journal of Nuts. 3, 23-28. https://doi.org/10.22034/JON.2012.515727
Heidarian R, Fotouhifar KB, Mohammadi AH, Javan-Nikkhah M, Debets AJM, Aanen DK (2015a) Host diversity and distribution of Paecilomyces formosus, the causal agent of pistachio die-back disease in Iran. In: Proceedings of Second Iranian Mycological Congress, University of Tehran, Karaj, Iran. pp. 210.
Heidarian R, Fotouhifar KB, Mohammadi AH, Javan-Nikkhah M, Debets AJM, Aanen DK (2015b) Phylogeny of genus Paecilomyces sp., the causal agent of pistachio die-back disease in Iran. In: Proceedings of Second Iranian Mycological Congress, University of Tehran, Karaj, Iran. pp. 30.
Marefat A, Ophel-Keller K, Scott ES, Sedgley M (2006) The use of ARMS PCR in detection and identification of Xanthomonads associated with pistachio dieback in Australia. Eur J Plant Pathol. 116(1), 57-68. https://doi.org/10.1007/s10658-006-9038-z
Michailides TJ, Rice RE, Ogawa JM (1987) Succession and significance of several hemipterans attacking a pistachio orchard. Journal of Economic Entomology. 80(2), 398-406. https://doi.org/10.1093/jee/80.2.398.
Mazaffari V, Malakouti MJ, Kholdebarin B, Bybordi M (2005) Investigation of several causal agents of pistachio dieback and its control with optimal nutrition. Iran Journal of Soil Research. 19(2), 154-164.
Mozaffari V, Malakouti MJ, Khold BB, Bybordi M (2005) Investigation of some causes of die-back disorder of pistachio trees and its control through balanced fertilization in Southern Iran. Iran Journal of Soil and Water Science. 19(2), 155-164.
Nazoori F, ZamaniBahramabadi E, Mirdehghan H, Yousefi M (2022) Preharvest application of sulfur as pesticide on fresh hull and kernel of pistachio (Pistacia vera L.). International Journal of Horticultural Science and Technology. 9(1), 117-129.
Norozi M, ValizadehKaji B, Karimi R, Nikoogoftar Sedghi M (2019) Effects of foliar application of potassium and zinc on pistachio (Pistacia vera L.) fruit yield. International Journal of Horticultural Science and Technology. 6(1), 113-123.
Sami MA, Alizadeh A, Saberi R (2005) Important pistachio pest and diseases in Iran and their integrated management. Iranian Student Book Agency, Tehran Branch. Iran.
Samson RA (1974) Paecilomyces and some allied Hyphomycetes. Studies in Mycology. 6, 1-119.
Samson RA, Houbraken J, Varga J, Frisvad JC (2009) Polyphasic taxonomy of the heat resistant ascomycete genus Byssochlamys and its Paecilomyces anamorphs. Persoonia. 2009; 22,14–27. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789542/ pmid: 20198134.
Sharifkhah M, Bakhshi D, Pourghayoumi M, Abdi S, Hokmabadi H (2020) Effect of pollination time on yield and antioxidant properties of some pistachio cultivars. International Journal of Horticultural Science and Technology. 7(1), 51-58.
Zarei-Jalalabadi A, Mohammadi S, Moradi M (2014) Evaluation of chemical control of Paecillomyces variotii, the causal agent of pistachio dieback. In third National Conference on Agriculture and Sustainable Development, Opportunities and Challenges, Islamic Azad University of Shiraz, Iran.