بررسی پارامترهای بیوشیمیایی و فعالیت آنزیم های آنتی اکسیدانی برنج تحت تاثیر تنش شوری

محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم

علی اصغر باقری ¹ ( گروه زیست شناسی،دانشگاه آزاد اسلامی واحد رودهن، )
فریبا خسروی نژاد ² ( گروه زیست شناسی، دانشگاه آزاد اسلامی واحد رودهن، )

تاریخ دریافت : 1395/07/03 تاریخ پذیرش : 1395/09/20 تاریخ انتشار : 1395/09/01

کلید واژه: تنش شوری, پرولین, Oryza sativa, آنزیم های آنتی اکسیدان, مالون دی آلدئید,

چکیده مقاله :

تنش های غیر زیستی از جمله شوری، مهم ترین عوامل محدود کننده رشد و عملکرد گیاهان زراعی در مناطق خشک و نیمه خشک هستند. بسیاری از اثرات تنش شوری در سطوح سلولی اعمال می گردد. به منظور درک پاسخ برنج به تنش شوری، فعالیت بعضی آنزیم های آنتی اکسیدان، پراکسیداسیون لیپید و محتوای پرولین مورد بررسی قرار گرفت. گیاه برنج به مدت 14 روز تحت تاثیر غلظت های 0، 100، 200و 300 میلی مولار کلرید سدیم(NaCl) قرار گرفت. بدین منظور، آزمایش گلخانه ای در قالب طرح بلوک های کامل تصادفی با 3 تکرار انجام گرفت. نتایج نشان داد که با افزایش کلرید سدیم پارامترهای رشد برای مثال وزن تر و خشک و اندازه گیاه کاهش نشان داد. فعالیت آنزیم های کاتالاز(CAT) و آسکوربات پراکسیداز(POD) با افزایش غلظت کلرید سدیم افزایش یافت اما فعالیت آنزیم سوپراکسید دیسموتاز(SOD) با افزایش غلظت کلرید سدیم تا غلظت 200 میلی مولار افزایش و سپس در غلظت 300 میلی مولار کاهش نشان داد. محتوای پرولین(Pro) و مالون دی آلدئید(MDA) در غلظت 300 میلی مولار به ترتیب به میزان 2 و 6/3 برابر نسبت به غلظت صفر میلی مولار کلرید سدیم افزایش نشان داد. نتایج ما نشان داد که افزایش فعالیت آنزیم های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز همراه با افزایش میزان پرولین درتحمل برنج به تنش شوری تاثیر مثبتی داشته است.

چکیده انگلیسی:

Abiotic stress including Salt stress is the major limiting factors of growth and crop production in arid and semiaridregions. Many of the Salinity affects are seen at the cellular levels. In order to understand the response of Oryza sativa to salt stress, some of antioxidant enzyme activities, Lipid peroxidation and Proline content were analyzed. Oryza sativa plant was treated by NaCl 0(control), 100, 200 and 300 mM for 14 days. For this purpose, a factorial design based on completely randomized design with three replications was used. The results showed that Growth parameters for example fresh and dry weight and height of shoot decreased under increasing NaCl. With increasing the concentration of NaCl, The activity of Catalase(CAT) and Ascorbat peroxidase(APX) were increased, but Superoxide dismutase (SOD) activity increased with increasing NaCl to 200 mM and then decreased at 300mM. Proline (Pro) and Malondialdehyde(MDA) contents respectively increased 2 and 3.6-fold at 300mM NaCl relative to the 0mM NaCl. Our results showed that by increasing the activity of CAT, SOD and APX associated with increased Proline had a positive effect on salt tolerance of Oryza sativa.

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