Effect of Foliar Application of Silicon on Physiological Responses of Chrysanthemum (Dendranthema ×grandiflorum) at Two Different Growth Stages
محورهای موضوعی : مجله گیاهان زینتیHadi Hajipour 1 , Zohreh Jabbarzadeh 2
1 - Department of Horticultural Science, College of Agriculture, Urmia University, P.O. Box 165, Iran
2 - Department of Horticultural Science, College of Agriculture, Urmia University, P.O. Box 165, Iran
کلید واژه: sodium silicate, ascorbate peroxidase, Catalase, Calcium silicate, Guaiacol peroxidase, hydrogen peroxide,
چکیده مقاله :
To evaluate the physiological responses of chrysanthemum to silicon spray in non-stress conditions, this study was performed in two separate experiments (at 4 to 5 and 8 to 12-leaf stages) as a factorial randomized completely design with two factors: silicon source (in two levels: sodium silicate and calcium silicate) and the concentration of silicate at five levels (0, 50, 100, 150 and 200 mg L-1) in cocopeat- perlite (1:1 v/v) medium in four replications and two observations. Physiological traits such as catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) activity, proline, malondialdehyde (MDA) and hydrogen peroxide (H2O2) content were measured. The results showed that the foliar spray of sodium and calcium silicate increased the activity of CAT, APX and GPX enzymes and MDA, but proline and H2O2 were decreased. Generally, in the stage of 8-12 leaves, sodium silicate at high concentrations (150 and 200 mg L-1) was the most effective in increasing antioxidant enzymes activity and reducing proline, MDA and H2O2. At 4-5 leaf stage sodium silicate at 100 mg L-1 increased antioxidant enzymes activity and calcium silicate at 150 mg L-1 reduced proline, MDA and H2O2.
این پژوهش برای ارزیابی واکنش های فیزیولوژیکی داوودی به محلولپاشی سیلیکون در شرایط بدون تنش انجام شد. این پژوهش به صورت دو آزمایش جداگانه (در مرحله 5-4 برگی و 12-8 برگی) به صورت فاکتوریل در قالب طرح کاملا تصادفی با دو فاکتور نوع سیلیکون (در دو نوع: سیلیکات سدیم و سیلیکات کلسیم) و غلظت سیلیکون (در 5 غلظت صفر، 50، 100، 150 و 200 میلی گرم در لیتر) در محیط کشت کوکوپیت- پرلیت (به نسبت حجمی 1:1) با 4 تکرار و 2 مشاهده انجام شد. ویژگی های فیزیولوژیکی نظیر فعالیت آنزیم های کاتالاز، آسکوربات پراکسیداز، گایاکول پراکسیداز، میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن اندازه گیری شدند. نتایج این پژوهش نشان داد که محلولپاشی با سیلیکات سدیم و کلسیم، میزان مالون دی آلدئید و فعالیت آنزیم های کاتالاز، آسکوربات پراکسیداز و گایاکول پراکسیداز را افزایش داد ولی پرولین و پراکسید هیدوژن کاهش یافتند. در کل، در مرحله 12-8 برگی، غلظت های زیاد سیلیکات سدیم (150 و 200 میلی گرم در لیتر) در افزایش فعالیت آنزیم های آنتی اکسیدانی و کاهش میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن موثرتر بود. در مرحله 5-4 برگی، سیلیکات سدیم با غلظت 100 میلی گرم در لیتر، فعالیت آنزیم‌های آنتی اکسیدانی را افزایش داد و سیلیکات کلسیم با غلظت 150 میلی گرم در لیتر، میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن را کاهش داد.
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