Chalcone Isomerase Gene Expression in Different Stages of Petunia Hybrida Flowering and Various Flower Colors
محورهای موضوعی : مجله گیاهان زینتیFatemeh Keykha Akhar 1 , Abdolreza Bagheri 2 , Nasrin Moshtaghi 3 , Masoud Fakhrfeshani 4
1 - Assistant Professor, Department of Plant Genetic and Production, Faculty of Agriculture, Jahrom University, Jahrom, Iran
2 - Professor, Department of Plant Biotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Associated Professor, Department of Plant Biotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
4 - Assistant Professor, Department of Plant Genetic and Production, Faculty of Agriculture, Jahrom University, Jahrom, Iran
کلید واژه: Real-time PCR, Naringenin, Chalcone isomerase, <i>Petunia hybrida</i>, Pigment pathway,
چکیده مقاله :
Petunia (Petunia hybrida) is one of the important plants in horticultural industry. This plant is being used as a model ornamental plant and is one of the most important plants in floriculture market. Flavonoids are the main pigment in this plant. So, genetic engineering with the goal of color alteration in petunia is focused on flavonoids. To gain a global perspective on genes differentially expressed in petunia’s flowers pigment pathway, we investigated the expression of chalcone isomerase (chi) as an essential gene in biosynthesis pathway of pigment production in different types of flower color of petunia and various stages of flowering in this plant. Also, we measured the concentration of total flavonoids, anthocyanins and naringenin to evaluate the probably relationship between the expression profile of chi gene and the concentration of mentioned pigments. The results indicated that chalcone isomerase expression had different profile in different petal color of P. hybrida. So that, the most chi expression observed in red petunia flowers. Naringenin concentrations was the most value in this color flower. In comparison of flowering stages, stage 1, had the most expression. In other words, when the flowers fully closed (bud stage), chi expression and concentration was in the highest value. Our results showed that chi is a key gene in pigment biosynthesis pathway so that in absence of this gene, pigment pathway will be stopped. Identification of effective genes in different pathways of secondary metabolite production will assist in accurate selection of genes for genetically modification of pathways and production of various metabolites.
اطلسی یکی از مهمترین گیاهان زینتی در صنعت گل و گیاهان زینتی است. این گیاه به عنوان یک گیاه مدل زینتی شناخته می شود و یکی از مهمترین گیاهان در بازار فروش گل می باشد. فلاونوئیدها یکی از اصلی ترین ترکیبات در این گیاه است. بنابراین مهندسی ژنتیک با هدف تغییر رنگ گل در اطلسی بر این ترکیبات متمرکز شده است. برای بدست آوردن نمای کلی از بیان ژن ها در مسیر بیوسنتزی گیاه اطلسی، ما بیان ژن چالکون ایزومراز را در رنگ های مختلف و مراحل متفاوت گلدهی در این گیاه بررسی کردیم. همچنین برای بدست آوردن رابطه احتمالی بین بیان ژن و میزان این ترکیبات، غلظت فلاونوئیدهای کل، آنتوسیانین ها و نارنجنین اندازه گیری شد. نتایج نشان داد که بیان ژن چالکون ایزومراز در رنگ های مختلف اطلسی متفاوت بود. بطوری که بیشترین بیان ژن در اطلسی با رنگ گل قرمز مشاهده شد. همچنین غلظت نارنجنین در این رنگ گل بیشترین مقدار بود. در مقایسه با مراحل مختلف گلدهی، مرحله 1 بیشترین بیان را داشت. به عبارت دیگر هنگامی که گل ها به طور کامل بسته بودند (غنچه گل)، بیان و غلظت چالکون ایزومراز بیشترین مقدار بود. نتایج آزمایش ما نشان داد که چالکون ایزومراز یکی از ژن های کلیدی در مسیر بیوسنتزی تولید رنگ در این گیاه است به طوری که در غیاب این ژن، تولید رنگ متوقف خواهد شد. شناسایی ژن های موثر در مسیر تولید متابولیت های ثانویه در انتخاب صحیح ژن ها برای تغییر ژنتیکی آن ها و تولید متابولیت های ثانویه مختلف موثر خواهد بود.
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