Proline and Arginine Improves the Vase Life of Cut Alstroemeria‘Mars’ Flowers by Regulating Some Postharvest Physiochemical Parameters
محورهای موضوعی : مجله گیاهان زینتی
Dina Yaghoobi Kiaseh
1
(
Ph.D. Student, Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
)
Davood Hashemabadi
2
(
Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
)
Behzad Kaviani
3
(
Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
)
کلید واژه: Senescence, amino acid, Proline, polyphenol oxidase, Protein demolition,
چکیده مقاله :
To study the effect of “cycloheximide” and amino acids of arginine and proline on post-harvest longevity of cut Alstroemeria × hybrid ‘Mars’ flowers, a factorial experiment was conducted based on a randomized complete design with 3 replications and 20 treatments. The experiment includes CHI at 4 levels (0, 50, 100, 1000 µM), amino acid (AA) at 5 levels [0, 5, 10 mM (Arg) and 5, 10 mM proline] in terms of 24 h pulse. The results showed that flowers treated with “CHI50 x Pro10” had the most vase-life (9.3 days), water uptake (1.771 ml g-1 F.W.), and dry material (13.56 %), the least MDA aggregation (1.32 nmol g-1 F.W.), and the most CAT enzyme activity (5.68 nmol g-1 F.W. min-1). The treatment of “CHI10 x Arg5” was one of the most successful treatments in water uptake, wet weight, dry material, and total protein preservation, and MDA aggregation decrease, SOD enzyme activity increase, and took the second place in vase-life increase (9.08 days). Therefore, the 2 treatments of “CHI0 x Arg5” and “CHI50 x Pro10” are presented as the most successful treatments in quantitative and qualitative trait’s improvement of cut Alstroemeria flowers. The lowest positive impact on the majority of analyzed traits belongs to the treatment of 1000 µM CHI. Therefore, the application of high levels of CHI (1000 µM) on vase-life solution of cut Alstroemeria × hybrid ‘Mars’ is not recommended due to negative and toxic impact on post-harvest traits.
بهمنظور بررسی اثر سیکلوهگزماید و اسیدهای آمینه آرژنین و پرولین بر ماندگاری پس از برداشت گلهای شاخه بریده آلسترومریا رقم ’مارس‘ آزمایشی فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار با 20 تیمار اجرا شد. در این آزمایش از دو فاکتور شامل سیکلوهگزماید (CHI) (0، 50، 100 و 1000 میکرومولار) و اسید آمینه (AA) ]0، 5 و 10 میلیمولار آرژنین (Arg) و 5 و 10 میلیمولار پرولین (Pro)[ بهصورت تیمار پالس 24 ساعته استفاده شد. نتایج نشان داد که گلهای تیمار شده با "CHI50×Pro10" بیشترین عمر گلجایی (3/9 روز)، بیشترین جذب آب (771/1 میلیلیتر در هر گرم وزن تر)، بیشترین ماده خشک (56/13 درصد)، کمترین تجمع مالوندی آلدهید (32/1 نانومول در هر گرم وزن تر) و بیشترین فعالیت آنزیم کاتالاز (68/5 نانومول در هر گرم وزن تر در دقیقه) را داشتند. تیمار "CHI0×Arg5" در حفظ جذب آب، وزن تر، ماده خشک، پروتئین کل، کاهش تجمع مالوندیآلدهید و افزایش فعالیت آنزیم سوپراکسید دیسموتاز از موفقترین تیمارها بود و رتبه دوم را در افزایش عمر گلجایی (08/9 روز) بخود اختصاص داد. بنابراین دو تیمار "CHI50×Pro10" و "CHI0×Arg5" بهعنوان موفقترین تیمارها در بهبود صفات کمی و کیفی گل شاخه بریده آلسترومریا معرفی میشوند. کمترین اثر مثبت در اکثر صفات ارزیابی شده متعلق به تیمار 1000 میکرومولار سیکلوهگزماید بود. بنابراین کاربرد سطوح بالای سیکلوهگزماید (1000 میکرومولار) در محلول گلجایی گلهای شاخه بریده آلسترومریا رقم ’مارس‘ به دلیل اثر منفی و سمی بر شاخصهای پس از برداشت توصیه نمیشود.
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