Detecting the Active Ingredients in the Extract and Essential Oil of the Ornamnetal and Medicinal Plant Species Rue (Ruta graveolens L.) and Studying their Antimicrobial Activity
محورهای موضوعی : مجله گیاهان زینتی
Fatemeh Torabi Chafjiri
1
(Ph.D. Student, Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran)
Davood Hashemabadi
2
(Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran)
Behzad Kaviani
3
(Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran)
Leila Asadpour
4
(Department of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran)
کلید واژه: GC-MS, Clevenger, disk diffusion, Acinetobacter baumannii, Microbial resistance,
چکیده مقاله :
The research aimed to explore the active ingredients of rue extract and essential oil as an ornamental and medicinal plant species and to assess their antimicrobial activity. So, rue shoots were collected from the natural habitat of this species in the elevations of Chaboksar, Guilan province in October and November 2019. The leaves and stems were oven-dried at 75 °C for 24 hours, and their extracts and essential oils were extracted with a Clevenger. The active ingredients of the extracts and essential oils were detected with GC-MS. The most abundant compounds in the leaf essential oil, stem essential oil, leaf extract, and stem extract were found to be hexadecanoic acid, isomaturnin, 2-ethyl-1,3,4,5,6,7,8-heptamethy, and 12-methoxy-19-norpodocarpa, respectively. The microbial assay by the disk diffusion method on the strains of Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, and Escherichia coli showed that the leaf and stem extracts were most influential in inhibiting A. baumannii. The assessment of MIC and MBC revealed that S. aureus was the most sensitive bacterium to the leaf and stem essential oils with the lowest MIC and MBC and A. baumannii was the most sensitive bacterium to the leaf extract with the lowest MBC. So, rue extract and essential oil can be used as an antimicrobial compound against human pathogenic bacteria.
این پژوهش با هدف بررسی مواد موثره موجود در عصاره و اسانس گیاه زینتی-دارویی سداب و بررسی اثر ضد میکروبی آن‌ها انجام شد. بدین‌منظور در آبان ماه 1398 اندام هوایی سداب از زیستگاه طبیعی آن در ارتفاعات چابکسر-استان گیلان جمع‌آوری شد. برگ‌ها و ساقه گیاه در آون الکتریکی با دمای 75 درجه سانتی‌گراد به مدت 24 ساعت خشک و سپس توسط کلونجر عصاره و اسانس گیاه استخراج شد. جهت شناسایی ترکیبات موثره عصاره و اسانس از GC-MS استفاده شد. نتایج نشان داد که Hexadecanoic acid، Isomaturnin، 2-Ethyl-1,3,4,5,6,7,8-heptamethy و12-methoxy-19-norpodocarpa به‌ترتیب بیشترین ترکیب موجود در اسانس برگ، اسانس ساقه، عصاره برگ و عصاره ساقه سداب است. نتایج تست میکروبی به روش دیسک دیفیوژن روی سویه‌های Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli نشان داد که عصاره برگ و ساقه سداب بیشترین اثر را در برابر مهار باکتری A. baumannii دارد. بررسی نتایج MIC و MBC نشان داد که باکتری S. aureus با کمترین MIC و MBC حساس‌ترین باکتری در برابر اسانس برگ و ساقه سداب است درحالی‌که A. baumannii با کمترین MBC بیشترین حساسیت را به عصاره برگ سداب نشان داد. بنابراین می‌توان از عصاره و اسانس گیاه سداب به-عنوان یک ماده ضد میکروبی علیه باکتری‌های بیماری‌زای انسانی استفاده نمود.
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