Mutation Induction in Chrysanthemum Cut Flowers Using Gamma Irradiation Method
محورهای موضوعی : مجله گیاهان زینتیMohammad Reza shafiei 1 , Abdollah Hatamzadeh 2 , Pejman Azadi 3 , Habibollah Samizadeh Lahiji 4
1 - PH.D. Student in Horticultuer Department of Horticultural Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran. P.O. Box: 41635-1314
2 - Department of Horticultural Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran. P.O. Box: 41635-1314
3 - Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Agricultural Research, Education and Extension Organization (AREEO), Iran| Ornamental Plant Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Iran
4 - Department of Agronomy Plant Breeding, Faculty of Agriculture Science, University of Guilan
کلید واژه: Gamma ray, Chrysanthemum, Mutagenesis, Radiation,
چکیده مقاله :
Chrysanthemum is one of the ornamental plants that cultivated widely in worldwide. This flower is one of the most important cut and pot flowers in the world due to its high diversity of colors and shapes. The creation of mutation is an important method for the production of new cultivars, and many cultivars have been produced through spontaneous and induced mutations. In this study, explants of leaf pieces from three most cultivated chrysanthemum varieties were irradiated with different gamma rays. This experiment was conducted as factorial in randomized complete block design with three replications. The results showed that an appropriate dose of gamma ray to produce a mutation in the varieties used in this experiment, is 25 G-ray. The obtained results showed that the 25 G-ray in purple cultivar produced the greatest change in petal color with a mutation rate of 54.56%. Meanwhile, the maximum number of new colors belonged to the purple group. Also, in the pink cultivar, the highest number of colored flowers was observed with a change of 32.11% in the 25 G-ray treatment. Based on the results of this study, four new cultivars will be introduced to the Iranian flower industry as a new cultivar.
داوودی یکی از انواع گیاهان زینتی است که به صورت بسیار گسترده‌ای در سطح جهان کشت می‌شود. این گل به لحاظ تنوع بالای رنگ و شکل دومین گل شاخه بریده و گلدانی در دنیا به شمار می‌آید. ایجاد موتاسیون روشی مهم برای تولید ارقام جدید است و ارقام بسیاری از طریق جهش‌های خودبه‌خودی و القاء شده تولید شده است. در این پژوهش ریزنمونه‌ی قطعات برگی از سه رقم مهم داوودی با دوزهای مختلف اشعه‌ی گاما مورد پرتوتابی قرار گرفتند. این آزمایش به صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی در 3 تکرار اجرا شد. بررسی نتایج نشان داد که دوز مناسب پرتو گاما، برای ایجاد جهش در ارقام مورد استفاده در این آزمایش 25 گری است. نتایج بدست آمده نشان داد که دوز 25 گری در رقم بنفش بیشترین تغییر رنگ گلبرگ‌ها با نرخ جهش 54/26 درصد را ایجاد کرد. در این بین بیشترین تعداد رنگ جدید متعلق به گروه بنفش کم‌رنگ بود. همچنین در رقم صورتی خالدار نیز بیشترین تعداد گل‌های تغییر رنگ یافته با مقدار تغییر 11/32 درصد در تیمار 25 گری مشاهد شد. بر اساس نتایج بدست آمده در این پژوهش چهار رقم جدید برای ارائه به صنعت گل ایران به عنوان رقم جدید معرفی خواهد شد.
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