In vitro Evaluation of Some Fungicides and Tea Extract Against Pestalotia sp. and Colletotrichum sp., The Causal Agents of Leaf Spot and Anthracnose of Azalea
محورهای موضوعی : مجله گیاهان زینتیMahsa Moshayedi 1 , Hadi Rahanandeh 2 , Alireza Hamzeh 3
1 - Young Researchers and Elite Club, Rasht Branch, Islamic Azad University
2 - Member of Department of Agronomy and Plant Breeding, Faculty of agriculture, Islamic Azad University, Rasht branch
3 - English language department, Arak branch, Islamic Azad University, Arak, Iran
کلید واژه: Guilan, Chemical fungicides, Fungal colony, <i>Rhododendron</i> spp, Tea extracts,
چکیده مقاله :
The effect of five fungicides and tea extracts was tested against anthracnose disease and leaf spot of Azalea during summer (2015) at the laboratory in Rasht Branch, Islamic Azad University. Commercially formulated fungicides at 1000, 2000 and 3000 ppm concentrations and the regression of normalized growth rate were used to determine the EC50. The application of different fungicides on Pestalotia sp., Bavistin 50WP, Dithane M-45, Aliette, Benlate 50 WP and Topsin M 70 WP gave significant reduction in colony growth, i.e. 0.5, 0.5, 0.50, 3.38 and 2.56 mm, respectively as compared to control (88.00 mm) in 2000 ppm at recommended doses. The application of different fungicides on Colletotrichum sp. Resulted in significant reduction in colony growth. The fungicides were tested against Colletotrichum sp. at 1000, 2000 and 3000 ppm concentrations, and no growth was observed in Mancozeb, Thiophanate-methyl and Fosetyl aluminum at any concentrations. Three of the tested fungicides suppressed mycelial pathogen growth effectively. However, there were significant differences in the sensitivities exhibited by the examined pathogen isolates. Concentrations of extracts from fresh tea leaves used to control the disease agents could not prevent the growth of fungal colony.
اثر پنج قارچ کش و عصاره برگ تازه چای در برابر لکه برگی و آنتراکنوز آزالیا در طول فصل تابستان 1394 در آزمایشگاه دانشکده کشاورزی دانشگاه آزاد واحد رشت مورد آزمایش قرار گرفت. قارچ کش ها در غلظت های 1000، 2000 و 3000 پی پی ام تهیه و به منظور تعیین درصد بازدارندگی از رشد عوامل بیماری زا به کار برده شدند. اثر قارچ کش های مختلف Bavistin 50WP، Dithane M-45، Aliette، Benlate 50 WP و Topsin M 70 WP روی قارچ Pestalotia کاهش قابل توجهی در رشد پرگنه در مقایسه با شاهد به ترتیب با 5/0، 5/0، 5/0 ، 38/3 و 56/2 میلی متر و شاهد 0/88 میلی متر در دوز تجاری توصیه شده 2000 پی پی ام داشت. قارچ کش های فوق کاهش قابل توجهی در رشد پرگنه Colletotrichum داشتند و در هر سه غلظت هیچ رشدی در مقابل سه قارچ کش Mancozeb ،Thiophanate-methyl وFosetyl aluminum مشاهده نشد. با این حال حساسیت قابل توجهی در پاتوژن های جدا شده به قارچ کش های مختلف وجود داشت. غلظت های مختلف عصاره برگ تازه چای مورد استفاده، برای کنترل عوامل بیماری زا فوق هیچ کدام قادر به جلوگیری از رشد کلنی قارچ ها در سطح آزمایشگاه نبودند.
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