Effects of Euphorbia hirta and Acidifiers Supplement on Resistance of Broiler Chickens against Salmonella enteritidis Infection: Oral Challenge Model
محورهای موضوعی : Camelس.ر. هاشمی 1 , آ. زولکیفلی 2 , ه. داوودی 3 , م. هیر-بجو 4 , ز. زونیتا 5
1 - Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 - Department of Animal Science, Faculty of Agriculture, University Putra Malaysia, Selangor, Malaysia
3 - Department of Microbiology and Immunology, Golestan University of Medical Science, Gorgan, Iran
4 - Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
5 - Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
کلید واژه: Broiler, herbal plant, organic acid, <i>Euphorbia hirta</i>, <i>Salmonella</i>,
چکیده مقاله :
The main purpose of this study was to investigate the effects of Euphorbia hirta and acidifiers supplement on resistance of broiler chickens against Salmonella enteritidis infection. A total of 120 day-old male broiler chicks were randomly assigned to 3 dietary treatments. 1) basal diet, 2) basal diet supplemented with 2 g/kg organic acid (OA) and 3) basal diet supplemented with 7.5 g/kg E. hirta (EH7.5). At d 3, all chicks were inoculated by 1 mL S. enteritidis (1.5×108 cfu/bird). Cloacal swabs were taken from the inoculated chicken on d 2, 10, 17, 23 and 28 post inoculation and the chicks were killed for microbiological examination on d 7, 14 and 28 after challenge. At seven and 14 days after challenge, the number of S. entritidis was significantly (P<0.05) higher in control group than the other groups and the rate of ceca enumeration dropped significantly (P<0.05) after 14 days in birds fed with OA compared to the EH7.5 diet. At 10 d after challenge, EH7.5 and OA treatments were significantly reduced in S. entritidis shedding compared with the control group (P<0.05). Cloacal S. entritidis shedding was not observed after 17 days post challenge in OA, as well as 23 days post challenge in OA and EH7.5 groups. In conclusion, this study clearly demonstrated that feeding of both EH7.5 and OA could successfully control S. enteritidis infection in broilers. However, based on S. entritidis cecal colonization and S. entritidis cloacal swabs, it is inferred that OA appeared to be more effective than EH7.5 inclusion in the diet on controlling salmonelosis in broilers.
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