Use of Microsatellite Polymorphisms in Ovar-DRB1 Gene for Identifying Genetic Resistance in Fat-Tailed Ghezel Sheep to Gastrointestinal Nematodes
محورهای موضوعی : Camelر. حاجی علیزاده ولیلو 1 , س.ع. رافت 2 , م. فیروزآمندی 3 , م. ابراهیمی 4
1 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 - Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
کلید واژه: gastrointestinal nematodes, Ghezel sheep, microsatellite polymorphisms, <i>Ovar-DRB1</i> gene,
چکیده مقاله :
This study was designed to identify genetically resistant animals to gastrointestinal nematode (GIN) infections using microsatellite polymorphisms of Ovar-DRB1 gene in Iranian Ghezel sheep breed lambs. In the present study 120 male Ghezel lambs were at 4 to 6 months of age randomly selected from six different sheep flocks in East Azerbaijan province (n=20 per flock). These lambs were naturally infected with GINs, and individual fecal samples were collected twice with a week interval to evaluate fecal egg counts (FEC). Blood samples were also collected for DNA isolation and PCR was performed to amplify the second exon and microsatellites within the second intron of the Ovar-DRB1 gene. The data were analyzed using a mixed modelof SAS software. The present study identified 24 genotypes and 20 alleles on Ovar-DRB1 gene. Results indicated that the presence of 510 bp (base pair) allele (called allele F) in both homozygote and heterozygote animals had a strong association (P<0.01) with lower FEC; while, presence of 506 bp allele (called allele E) in homozygote animals was significantly associated (P<0.01) with higher FEC. Thus, this study showed a strong association between microsatellite polymorphism of Ovar-DRB1 gene and resistance to GIN infections in Ghezel sheep lambs.
این پژوهش به منظور شناسایی دامهای دارای مقاومت ژنتیکی نسبت به نماتودهای دستگاه گوارش (GIN) با استفاده از چندشکلیهای ریزماهوارهای موجود در ژن Ovar-DRB1 برههای نژاد قزل ایرانی طراحی شد. در پژوهش حاضر 120 بره نر قزل 4 تا 6 ماهه به صورت تصادفی از 6 گله گوسفند متفاوت در استان آذربایجان شرقی انتخاب شدند (تعداد 20 گوسفند در هر گله). این برهها به صورت طبیعی با GINها آلوده شده و نمونههای مدفوعی هر حیوان نیز جهت شمارش تعداد تخمانگل موجود در مدفوع (FEC) به صورت دوبار در هفته و با یک هفته فاصله جمعآوری شد. نمونههای خونی نیز جهت استخراج DNA جمعآوری شدند و PCR به منظور افزودهسازی اگزون شماره 2 و توالیهای ریزماهوارهای موجود در اینترون شماره2 ژن Ovar-DRB1 انجام شد. دادهها با استفاده از رویه مختلط در نرم افزار SAS آنالیز شدند. در پژوهش حاضر 24 ژنوتیپ و 20 آلل برای ژن Ovar-DRB1 شناسایی شدند. نتایج نشان دادند که حضور آللی به طول 510 جفت باز (که آلل F نامیده شد) در هر دو دامهای هموزیگوت و هتروزیگوت دارای رابطه قابلتوجه معنیداری (01/0P<) با کاهش FEC دارد، در حالیکه حضور آللی به طول 506 جفتباز (که آلل E نامیده شد) در دامهای هموزیگوت نیز دارای رابطه معنیداری (01/0P<) با افزایش میزان FECمی باشد. بنابراین این پژوهش رابطه قابلتوجه معنیداری را بین چندشکلیهای ریزماهوارهای موجود در ژن Ovar-DRB1 و مقاومت به GIN در برههای نژاد قزل نشان داد.
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