Effect of Different Progesterone Protocol and Low Doses of Equine Chorionic Gonadotropin (eCG) on Oestrus Synchronization in Arabian Ewes
محورهای موضوعی : Camelف. خلیلاوی 1 , م. ممویی 2 , ص. طباطبایی 3 , م. چاجی 4
1 - Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
2 - Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
3 - Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
4 - Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
کلید واژه: reproductive performance, Arabic ewes, MAP sponge, anoestrus season,
چکیده مقاله :
This experiment was conducted to determine the reproductive performance of Arabian ewes treated with short and long-term progesterone devices in addition to low doses of equine chorionic gonadotropin (eCG) during the anoestrus season. A total of 36 ewes were divided into three groups: in group I vaginal sponges (60 mg medroxy progesterone acetate; (MAP)) were applied and removed after 6 days; in group II, vaginal MAP sponges were removed 12 days following insertion, while group III served as control group. The first two groups were intramuscularly injected with of 300 IU eCG, following sponge removal. Parameters such as oestrus response rate, time to onset of oestrus, duration of oestrus, pregnancy, lambing and fecundity rates were evaluated. Blood samples were collected one day before sponge insertion and two days after sponge insertion and on day of oestrus. There were significant differences between the group I and II with control group regarding the plasma estradiol and progesterone levels. There were no significant differences in oestrus response, time to onset of oestrus, pregnancy, lambing and fecundity rates between groups I and II (P>0.05). However, differences were significant when these two treatment groups were compared with the control group. In group I, duration of oestrus was significantly higher than group II (P<0.05). In addition, other factors in group I was numerically greater than group II (P>0.05). It was concluded that short-term sponge treatment (6 days) had better performance when compared with the long-term sponge treatment (12 days) in Arabian ewes.
این آزمایش به منظور ارزیابی عملکرد تولیدمثلی میشهای عربی تیمار شده با اسفنج‌های پروژسترونی کوتاه و بلندمدت به همراه سطح پایینی از هورمون eCG، در فصل غیر تولیدمثلی انجام گرفت. بدین منظور 36 رأس میش به سه گروه تقسیم‌بندی شدند: در گروه اول و دوم اسفنج‌های پروژسترونی (60 میلی‌گرم پروژسترون استات، MAP) به ترتیب به مدت 6 و 12 روز به کار گرفته شدند، در حالیکه گروه سوم (بدون هیچ تیمار هورمونی) به عنوان گروه کنترل در نظر گرفته شد. دو گروه ابتدایی پس از حذف اسفنج پروژسترونی، 300 واحد هورمون eCG به صورت تزریق عضلانی دریافت کردند. پارامترهایی مانند نرخ پاسخ فحلی، زمان شروع فحلی، طول مدت فحلی، آبستنی، بره‌زایی و میزان تزایدگله مورد ارزیابی قرار گرفتند. نمونه‌های خون در یک روز قبل از قرارگیری اسفنج، دو روز پس از قرار دادن اسفنج و در روز فحلی گرفته شد. اختلاف آماری معنی‌داری در میان گروه اول و دوم با گروه کنترل بر اساس سطوح استروژن و پروژسترون وجود داشت. اختلاف آماری معنی‌داری در نرخ پاسخ فحلی، زمان شروع فحلی، طول مدت فحلی، آبستنی، بره‌زایی و نرخ تزاید گله در میان گروه اول و دوم وجود نداشت (05/0<P). هرچند این اختلافات زمانی که این دو گروه با گروه کنترل مقایسه گردیدند، معنی‌دار بود. در گروه اول، طول مدت فحلی به طور معنی‌داری از گروه دوم بالاتر بود (05/0>P). به‌‌‌نظر می‌رسدکه در میشهای عربی تیمار اسفنج کوتاه‌مدت (6 روزه) دارای عملکرد بهتری در مقایسه با تیمار اسفنج بلندمدت (12 روزه) بوده است.
Akoz M., Bulbul M., Ataman B. and Dere S. (2006). Induction of multiple births in Akkaraman cross-breed synchronized with short duration and different doses of progesterone treatment combined with PMSG outside the breeding season. Bull. Vet. Inst. Pulawy. 50, 97-100.
Amer H.A. and Hazzaa A.M. (2009). The effect of different progesterone protocols on the reproductive efficiency of ewes during the non-breeding season. Vet. Arhiv. 79, 19-30.
Ataman M.B., Akoz M. and Akman O. (2006). Induction of synchronized oestrus in Akkaraman cross-bred ewes during breeding and anestrus seasons: the use of short and long-term progesterone treatments. Rev. Med. Vet. 50, 257-260.
Bacha S., Khiati B., Hammoudi S.M., Kaidi R. and Ahmed M. (2014). The effects of dose of pregnant mare serum gonadotropin (PMSG) on reproductive performance of algerian Rembi ewes during seasonal anoestrus. J. Vet. Sci. Technol. 5, 190-198.
Bastan A. (1995). Effect of melatonin and progesterone application on reproductive performance in akkaraman ewes (Akkaraman ırkı koyunlarda melatonin ve progesterone uygulamalarının reprodu¨ktif performans u¨zerine etkileri). Ph D. Thesis. AnkaraUniv., Ankara, Turkey.
Beck N.F.G., Davies B. and Williams S.P. (1993). Oestrous synchronization in ewes-the effect of combining a prostaglandin analogue with a 5-day progesterone treatment. Anim. Prod. 56, 207-210.
Błaszczyk B., Udała J. And Gaczarzewicz D. (2004). Changes in estradiol, progesterone, melatonin, prolactin and thyroxine concentrations in blood plasma of goats following induced estrus in and outside the natural breeding season. Small Rumin. Res. 51, 209-219.
Chanvallon A., Blache D., Chadwick A., Esmaili T., Hawken P.A.R., Martin G.B,. Vinoles C. and Fabre-Nys C. (2008). Sexual experience and temperament affect the response of Merino ewes to the ram effect during the anoestrous season. Anim. Reprod. Sci. 37, 1110-1016.
Emsen E. and Yaprak M. (2006). Effect of controlled breeding on the fertility of Awassi and Red Karaman ewes and the performance of the off spring. Small Rumin. Res. 66, 230-235.
Evans A.C., Flynn J.D., Quinn K.M., Daffy P., Quinn P. and Madgwick S. (2001). Ovulation of aged follicles does not affect embryo quality or fertility after a 14-day progesterone estrus synchronization protocol in ewes. Theriogenology. 56, 923-936.
Fajt V.R. (2011). Drug laws and regulations for sheep and goats. Vet. Clin. North America. Food Anim. 27, 1-21.
Freitas V.J.F., Baril G. and Saumande J. (1996). Induction and synchronization of estrus in goats: the relative efficiency of one versus two fluorogestone acetate-impregnated vaginal sponges. Theriogenology. 46, 1251-1256.
Godfrey R.W., Collins J.R., Hensley E.L. and Wheaton J.E. (1999). Oestrus synchronization and artificial insemination of hair sheep ewes in the tropics. Theriogenology. 51, 985-997.
Greyling J.P.C., Kotze W.F., Taylor G.J., Hangendijk W.J. and Cloete F. (1994). Synchronization of oestrus in sheep: use of different doses of progesterone outside the normal breeding season. South African J. Anim. Sci. 24, 33-37.
Greyling J.P.C. and Van der Nest M. (2000). Synchronization of oestrus in goats: dose effect of progesterone. Small Rumin. Res. 36, 201-207.
Hashemi M., Safdarian M. and Kafi M. (2006). Estrous response to synchronization of estrus using different progesterone treatments outside the natural breeding season in ewes. Small Rumin. Respon. 65, 279-283.
Husein M.Q. and Haddad S.G. (2006). A new approach to enhance reproductive performance in sheep using royal jelly in comparison with equine chorionic gonadotropin. Anim. Reprod. Sci. 93, 24-33.
Husein M.Q., Kridli R.T. and Humphrey W.D. (1999). Effect of royal jelly onestrus synchronization and pregnancy rate of ewes using flourogestone acetate sponges. J. Anim. Sci. 77(1), 431-438.
Husein M.Q. and Kridli R.T. (2002). Reproductive responses following royal jelly treatment administered orally or intramuscularly into progesterone-treated Awassi ewes. Anim. Reprod. Sci. 74(2), 45-53.
Iida K., Kobayashi N., Kohno H., Miyamoto A. and Fukui Y. (2004). A comparative study of induction of estrus and ovulation by three different intravaginal devices in ewes during the non-breeding season. J. Reprod. Develop. 50, 63-69.
Knights M., Maze T.D., Bridges P.J., Lewis P.E. and Inskep E.K. (2001). Short-term treatment with a controlled internal drug releasing (CIDR) device and FSH to induce fertile estrus and increase prolificacy in anestrus ewes. Theriogenology. 55, 1181-1191.
Koyuncu M. and Alticekic S. (2010). Effects of progesterone and PMSG on estrous synchronization and fertility in Kivircik ewes during natural breeding season. Asian Australas J. Anim. 23, 308-311.
Kridli R.T. and Al-Khetib S.S. (2006). Reproductive responses in ewes treated with eCG or increasing doses of royal jelly. Anim. Reprod. Sci. 92, 75-85.
Menegatos J., Chadio S. and Kauskoura T. (2003). Endocrine event during the pre estruos period and the subsequent estrous cycle in ewes after estrous synchronization. Theriogenology. 59, 1533-1543.
Moghaddam G.H., Olfati A., Daghigh Kia H. and Rafat S.A. (2012). Study of reproductive performance of crossbred ewes treated with GnRH and PMSG during breeding season. Iranian J. Appl. Anim. Sci. 2(4), 351-356.
Moradi Kor N. and Ziaei N. (2012). Effect of PGF2 administration and subsequent eCG treatments on the reproductive performance in mature Raieni goats during the breeding season. Asian J. Anim. Vet. Adv. 7, 94-99.
Nasser S.O., Wahid H., Aziz A.S., Zuki A.B., Azam M.K., Jabbar A.G. and Mahfoz M.A. (2012). Effect of different oestrus synchronizations protocols on the reproductive efficiency of Dammar ewes in Yemen during winter. African J. Biotechnol. 11, 9156-9162.
Noel B., Bister J.L., Pierquin B. and Paquay R. (1994). Effect of FGA and PMSG on follicular growth and LH secretion in Suffolk ewes. Theriogenology. 41, 719-727.
Ozturkler Y., Colak A., Baykal A. and Guven B. (2003). Combined effect of a prostaglandin analogue and a progesterone treatment for 5 days on oestrus synchronization in Tushin ewes. Indian Vet. J. 80, 917-920.
Ozyurtlu N., AY S.S., KucukaslanI., Gungor O. and Aslan S. (2011). Effect of subsequent two short-term, short-term and long-term progesterone treatments on fertility of Awassi ewes out of the breeding season. Ankara Univ. Vet. Fak. Derg. 58, 105-109.
Ozyurtlu N., KucukaslanI. and Cetin Y. (2010). Characterization of oestrous induction response, oestrous duration, fecundity and fertility in Awassi ewes during the non-breeding season utilizing both CIDR and intravaginal sponge treatments. Reprod. Domest. Anim. 4, 464-467.
Robinson T.J., Moore N.W., Holst P.J. and Smith J.F. (1967). The evaluation of several progestogens administered in intravaginal sponges for the synchronization of estrus in the entire cyclic Merino ewe. Pp. 76-91 in Control of the Ovarian Cycle in the Sheep. T.J. Robinson, Ed. White and Bull PTY Ltd., Australia.
Romano J.E. (1996). Comparison of fluorgestone and medroxyprogesterone intravaginal pessaries for estrus synchronization in dairy goats. Small Rumin. Res. 22, 216-223.
Romano J.E. (1998). The effect of continuous presence of bucks on hastening the onset of estrus in synchronized does during the breeding season. Small Rumin. Res. 30, 99-103.
Sanchez E.J. (2005). Melatonin-estrogen interaction in breast cancer. J. Pineal. Res. 38, 217-222.
Saribay M.K., Karaca F., Dogruer G., Ergun Y., Yavas I. and Ates C.T. (2011). Oestrus synchronization by short and long-term intravaginal sponge treatment in lactating goats during the breeding season: the effects of GnRH administrations immediately after matings on fertility. J. Anim. Vet. Adv. 10, 3134-3139.
SAS Institute. (1998). SAS®/STAT Software, Release 6. SAS Institute, Inc., Cary, NC. USA.
Timurkan H. and Yildiz H. (2005). Synchronization of oestrus in Hamedani ewes: the use of different PMSG dose. Bull. Vet. Pulawy. 49, 311-314.
Ungerfeld R. and Rubianes E. (2002). Short term primings with different progestogen intravaginal devices (MAP, FGA and CIDR) for eCG oestrous induction in anestrus ewes. Small Rumin. Res. 46, 63-66.
Ustuner B., GunayU., NurU. and UstunerU. (2007). Effects of long and short-term progestogens treatment combined with PMSG on oestrus synchronization and fertility in Awassi ewes during the breeding season. J. Acta Vet. Brno. 76, 391-397.
Vinoles C., Forsberg M., Banchero G. and Rubianes E. (2001). Effect of long-termand short termprogesterone treatment on follicular development and preganancy rate in cyclic ewes. Theriogenology. 55, 993-1001.
Vinoles C., Meikle A., Forsberg M. and Rubianes E. (1999). The effect of subluteal levels of exogenous progesterone on follicular dynamics and endocrine patterns during the early luteal phase of the ewe. Theriogenology. 51, 1351-1361.
Wildeus S. (1999). Current concepts in synchronization of oestrus: sheep and goats. Proc. Am. Soc. Anim. Sci. 39, 1-14.
Zeleke M., Greyling L.M.J. and Schwalbach T. (2005). Effect of progesterone and PMSG on oestrous synchronization and fertility in dorper ewes during the transition period. Small Rumin. Res. 56(1), 47-53.
Zonturlu A.K., Aral F., Ozyurtlu N. and Yavuzer U. (2008). Syncronization of estrous using FGA and CIDR intervaginal pessaries during the transition period in Awassi ewes. J. Anim. Vet. Adv. 7, 1093-1069.
