Lack of Association between Somatotropin Receptor Gene Polymorphism and Birth Weight of Iranian Indigenous Sistani Cattle

محورهای موضوعی : Camel

آ. جوانمرد ¹ , س. ر. میرایی آشتیانی ² , آ. ترکمن زهی ³ , م. مرادی شهر بابک ⁴

1 - Department of Animal Science, University of Tehran, Karaj, Iran
2 - Department of Animal Science, University of Tehran, Karaj, Iran
3 - Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran
4 - Department of Animal Science, University of Tehran, Karaj, Iran

تاریخ دریافت : 1391/10/27 تاریخ پذیرش : 1392/01/27 تاریخ انتشار : 1392/09/10

کلید واژه: PCR-RFLP, candidate gene, indigenous Sistani cattle, somatotropin receptor genes,

چکیده مقاله :

The objective of the present study was to determine polymorphism within the promoter region of somatotropin receptor genes in indigenous Sistani cattle (Bos indicus) and associations between this polymorphism and breeding value of birth weight. The pedigree structure was included by considering 1173 animals with 600 progeny birth weight data obtained from a Zhark breeding station in Sistan and Baluchistan. Heritability was estimated for birth weight using different univariate models with the derivative-free approach of restricted maximum likelihood algorithm (DFREML). The average weight of each birth was 23.9 ± 3.16 kg. The effects of non-genetic factors were significant (P<0.01) for birth weight. Direct heritabilities (h2) in single trait analyses were 0.31 ± 0.06. Genomic DNA was isolated from blood and semen using conventional methods. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays were used to genotype this candidate gene in 72 individuals with the birth weight record. The observed allele size was similar to that reported in the literature. The Sistani cattle showed higher frequency of alleles ALuI (+) than ALuI (-) in population. Statistical analysis was conducted to test the association of this polymorphism with the breeding value of birth weight data. There was not significant association between producing genotypes and birth weight trait. Future research on another candidate gene and growth trait strongly is encouraged to deep in the understanding of growth pattern in this breed.

چکیده انگلیسی:

تحقیق حاضر برای بررسی ارتباط چند‌شکلی موجود در ناحیه پروموتور ژن‌ گیرنده‌ رشد با وزن ‌تولد در گاوهای سیستانی انجام شد. به این منظور ساختار شجره موجود، با تعداد کل 1173 حیوان و 600 گوساله حاوی رکورد دقیق وزن ‌تولد، متعلق به ایستگاه اصلاح‌نژاد زهک سیستان و بلوچستان مورد مطالعه قرار گرفت. در گام اولیه برآورد مولفه‌های واریانس ژنتیکی و وراثت‌پذیری وزن ‌تولد و با استفاده از مدل حیوانی مناسب و روش حداکثر درستنمایی محدود شده و به طریق تک صفته مورد برآورد قرار گرفت. میانگین وزن ‌تولد در گاو سیستانی صرفه ‌نظر از لحاظ جنسیت گوساله، حدود 16/3 ± 9/23 کیلو‌گرم برآورد شد. البته این برآورد، با تصحیح اثر عوامل ثابت مانند جنسیت و تعداد شکم بر روی این صفت صورت گرفت. وراثت‌پذیری وزن ‌تولد 06/0 ± 31/0 برآورد شد. استخراج دی‌ان‌آ، از خون کامل ورید وداجی و اسپرم‌های موجود به روش‌های متفاوت صورت پذیرفته و سپس چند شکلی قطعات حاصل از هضم آنزیمی متعاقب پی‌سی‌آر بر روی این ژن کاندیدا مورد بررسی قرار گرفت. نتایج حاصل از این تحقیق نشان داد اندازه قطعات حاصل از پی‌سی‌آر و هضم آنزیمی با تحقیقات مشابه همخوانی داشته و پس از محاسبه فراوانی ژنوتیپی و اللی، سه ژنوتیپ مشاهده شده هیچ ارتباط معنی‌داری را با وزن تولد نشان ندادند. بررسی چند شکلی ناحیه‌های دیگر ژن کاندیدای مذکور یا سایر ژن‌های مرتبط با رشد برای درک مکانیزم کنترل کننده رشد در گاو سیستانی پیشنهاد می‌شود.

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