Joint Analysis of the DGAT1, OPN and PPARGC1A Genes Effects on Variation of Milk Production and Composition in Holstein Cattle Population
محورهای موضوعی : Camel
ح. خراتی کوپایی
1
(Institute of Biotechnology, Shiraz University, Shiraz, Iran)
م. پسندیده
2
(Department of Genetics and Animal Breeding, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari, Iran)
م. دادپسند
3
(Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran)
ع. اسماعیلی‌زاده کشکوئیه
4
(Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran)
م. ر. محمدآبادی
5
(Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran)
کلید واژه: Holstein, <i>DGAT1</i>, <i>OPN</i>, <i>PPARGC1A</i>,
چکیده مقاله :
The aim of this study was to investigate effects of DGAT1, OPN and PPARGC1A candidate genes on milk production traits in Iranian Holstein cattle. Several papers have studied single nucleotide polymorphisms (SNPs) and their association with economic traits in dairy cows, but the combined effect of these genes has not been examined in Iranian Holstein cattle population. Blood samples were collected from 398 registered Holstein cows. Total DNA was extracted using the salting out protocol. The PCR-RFLP technique was used for SNPs genotyping. The largest genotype frequency was estimated as 0.65 for PPARGC1A (c.1892)CT and the least frequency was estimated as 0.09 for DGAT1KK genotype. The allele frequencies were in the range 0.36 to 0.64 for PPARGC1A (c.3359)A and Calleles,respectively. The allelic substitution effects were estimated using a multiple regression model. The effects of allelic substitution for DGAT1K and PPARGC1A (c.1892)T were significant on estimated breeding values for fat percentage (EBVFP) (P<0.01). In addition, the results of multivariate analysis indicated the significant effect of DGAT1 and PPARGC1A (c.1892) on EBVFP (P<0.05). However, there were no association between OPN and PPARGC1A (c.3359) polymorphisms and studied traits.
این پژوهش با هدف بررسی ارتباط ژن‏های کاندیدای DGAT1، OPN و PPARGC1A با صفات تولید شیر در جمعیت گاوهای هلشتاین ایران انجام شد. مقالات زیادی چندشکلی‏ها و ارتباط آن‏ها را با صفات اقتصادی در گاوهای شیری مورد تحقیق قرار داده‏اند اما تاکنون اثر ترکیبی این ژن‏ها در گاوهای هلشتاین ایران بررسی نشده است. نمونه‏های خون از 398 رأس گاو هلشتاین تهیه شدند. استخراج DNA با روش نمکی انجام شد. از تکنیک PCR-RFLP برای تعیین ژنوتیپ SNP­ها استفاده شد. بیشترین فراوانی ژنوتیپی به اندازه 65/0 برایPPARGC1A (c.1892)CT و کمترین فراوانی ژنوتیپی به اندازه 09/0 برای DGAT1KK به دست آمد. فراوانی‏های آللی در دامنه 36/0 تا 64/0 به ترتیب برای آلل‏های A و C در جایگاه PPARGC1A (c.3359) تعیین شد. اثرات جایگزینی آللی با استفاده از مدل رگرسیون چندگانه تخمین زده شد. اثرات جایگزینی آللی ژن‏های DGAT1K و PPARGC1A (c.1892)Tبرای ارزش‏های اصلاحی درصد چربی شیر در سطح 01/0 معنی‏دار بودند. همچنین نتایج آنالیز چند متغیره اثر معنی‏دار DGAT1 و PPARGC1A (c.1892) روی ارزش‏های اصلاحی درصد چربی شیر را در سطح 05/0 نشان داد. اما ارتباطی بین چندشکلی‏های OPN و PPARGC1A (c.3359) با صفات مورد مطالعه وجود نداشت.
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