Joint Analysis of the DGAT1, OPN and PPARGC1A Genes Effects on Variation of Milk Production and Composition in Holstein Cattle Population
الموضوعات :ح. خراتی کوپایی 1 , م. پسندیده 2 , م. دادپسند 3 , ع. اسماعیلیزاده کشکوئیه 4 , م. ر. محمدآبادی 5
1 - Institute of Biotechnology, Shiraz University, Shiraz, Iran
2 - Department of Genetics and Animal Breeding, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran
4 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
5 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
الکلمات المفتاحية: Holstein, <i>DGAT1</i>, <i>OPN</i>, <i>PPARGC1A</i>,
ملخص المقالة :
The aim of this study was to investigate effects of DGAT1, OPN and PPARGC1A candidate genes on milk production traits in Iranian Holstein cattle. Several papers have studied single nucleotide polymorphisms (SNPs) and their association with economic traits in dairy cows, but the combined effect of these genes has not been examined in Iranian Holstein cattle population. Blood samples were collected from 398 registered Holstein cows. Total DNA was extracted using the salting out protocol. The PCR-RFLP technique was used for SNPs genotyping. The largest genotype frequency was estimated as 0.65 for PPARGC1A (c.1892)CT and the least frequency was estimated as 0.09 for DGAT1KK genotype. The allele frequencies were in the range 0.36 to 0.64 for PPARGC1A (c.3359)A and Calleles,respectively. The allelic substitution effects were estimated using a multiple regression model. The effects of allelic substitution for DGAT1K and PPARGC1A (c.1892)T were significant on estimated breeding values for fat percentage (EBVFP) (P<0.01). In addition, the results of multivariate analysis indicated the significant effect of DGAT1 and PPARGC1A (c.1892) on EBVFP (P<0.05). However, there were no association between OPN and PPARGC1A (c.3359) polymorphisms and studied traits.
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