Joint Analysis of the DGAT1, OPN and PPARGC1A Genes Effects on Variation of Milk Production and Composition in Holstein Cattle Population
محورهای موضوعی : Camelح. خراتی کوپایی 1 , م. پسندیده 2 , م. دادپسند 3 , ع. اسماعیلی‌زاده کشکوئیه 4 , م. ر. محمدآبادی 5
1 - Institute of Biotechnology, Shiraz University, Shiraz, Iran
2 - Department of Genetics and Animal Breeding, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran
4 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
5 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
کلید واژه: Holstein, <i>DGAT1</i>, <i>OPN</i>, <i>PPARGC1A</i>,
چکیده مقاله :
The aim of this study was to investigate effects of DGAT1, OPN and PPARGC1A candidate genes on milk production traits in Iranian Holstein cattle. Several papers have studied single nucleotide polymorphisms (SNPs) and their association with economic traits in dairy cows, but the combined effect of these genes has not been examined in Iranian Holstein cattle population. Blood samples were collected from 398 registered Holstein cows. Total DNA was extracted using the salting out protocol. The PCR-RFLP technique was used for SNPs genotyping. The largest genotype frequency was estimated as 0.65 for PPARGC1A (c.1892)CT and the least frequency was estimated as 0.09 for DGAT1KK genotype. The allele frequencies were in the range 0.36 to 0.64 for PPARGC1A (c.3359)A and Calleles,respectively. The allelic substitution effects were estimated using a multiple regression model. The effects of allelic substitution for DGAT1K and PPARGC1A (c.1892)T were significant on estimated breeding values for fat percentage (EBVFP) (P<0.01). In addition, the results of multivariate analysis indicated the significant effect of DGAT1 and PPARGC1A (c.1892) on EBVFP (P<0.05). However, there were no association between OPN and PPARGC1A (c.3359) polymorphisms and studied traits.
این پژوهش با هدف بررسی ارتباط ژنهای کاندیدای DGAT1، OPN و PPARGC1A با صفات تولید شیر در جمعیت گاوهای هلشتاین ایران انجام شد. مقالات زیادی چندشکلیها و ارتباط آنها را با صفات اقتصادی در گاوهای شیری مورد تحقیق قرار دادهاند اما تاکنون اثر ترکیبی این ژنها در گاوهای هلشتاین ایران بررسی نشده است. نمونههای خون از 398 رأس گاو هلشتاین تهیه شدند. استخراج DNA با روش نمکی انجام شد. از تکنیک PCR-RFLP برای تعیین ژنوتیپ SNPها استفاده شد. بیشترین فراوانی ژنوتیپی به اندازه 65/0 برایPPARGC1A (c.1892)CT و کمترین فراوانی ژنوتیپی به اندازه 09/0 برای DGAT1KK به دست آمد. فراوانیهای آللی در دامنه 36/0 تا 64/0 به ترتیب برای آللهای A و C در جایگاه PPARGC1A (c.3359) تعیین شد. اثرات جایگزینی آللی با استفاده از مدل رگرسیون چندگانه تخمین زده شد. اثرات جایگزینی آللی ژنهای DGAT1K و PPARGC1A (c.1892)Tبرای ارزشهای اصلاحی درصد چربی شیر در سطح 01/0 معنیدار بودند. همچنین نتایج آنالیز چند متغیره اثر معنیدار DGAT1 و PPARGC1A (c.1892) روی ارزشهای اصلاحی درصد چربی شیر را در سطح 05/0 نشان داد. اما ارتباطی بین چندشکلیهای OPN و PPARGC1A (c.3359) با صفات مورد مطالعه وجود نداشت.
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