آنالیز ترکیبی ژن‌های DGAT1، OPN و PPARGC1A برای صفات تولید و ترکیب شیر در جمعیت گاوهای هلشتاین ایران

Subject Areas : Camel

ح. خراتی کوپایی ¹ ( Institute of Biotechnology, Shiraz University, Shiraz, Iran )
م. پسندیده ² ( Department of Genetics and Animal Breeding, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari, Iran )
م. دادپسند ³ ( Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran )
ع. اسماعیلی‌زاده کشکوئیه ⁴ ( Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran )
م. ر. محمدآبادی ⁵ ( Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran )

Received: 2016-02-10 Accepted : 2016-06-15 Published : 2016-12-01

Keywords: هلشتاین, <i>DGAT1</i>, <i>OPN</i>, <i>PPARGC1A</i>,

Abstract :

این پژوهش با هدف بررسی ارتباط ژن‏های کاندیدای DGAT1، OPN و PPARGC1A با صفات تولید شیر در جمعیت گاوهای هلشتاین ایران انجام شد. مقالات زیادی چندشکلی‏ها و ارتباط آن‏ها را با صفات اقتصادی در گاوهای شیری مورد تحقیق قرار داده‏اند اما تاکنون اثر ترکیبی این ژن‏ها در گاوهای هلشتاین ایران بررسی نشده است. نمونه‏های خون از 398 رأس گاو هلشتاین تهیه شدند. استخراج DNA با روش نمکی انجام شد. از تکنیک PCR-RFLP برای تعیین ژنوتیپ SNP­ها استفاده شد. بیشترین فراوانی ژنوتیپی به اندازه 65/0 برایPPARGC1A (c.1892)CT و کمترین فراوانی ژنوتیپی به اندازه 09/0 برای DGAT1KK به دست آمد. فراوانی‏های آللی در دامنه 36/0 تا 64/0 به ترتیب برای آلل‏های A و C در جایگاه PPARGC1A (c.3359) تعیین شد. اثرات جایگزینی آللی با استفاده از مدل رگرسیون چندگانه تخمین زده شد. اثرات جایگزینی آللی ژن‏های DGAT1K و PPARGC1A (c.1892)Tبرای ارزش‏های اصلاحی درصد چربی شیر در سطح 01/0 معنی‏دار بودند. همچنین نتایج آنالیز چند متغیره اثر معنی‏دار DGAT1 و PPARGC1A (c.1892) روی ارزش‏های اصلاحی درصد چربی شیر را در سطح 05/0 نشان داد. اما ارتباطی بین چندشکلی‏های OPN و PPARGC1A (c.3359) با صفات مورد مطالعه وجود نداشت.

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