Effect of Different Harvesting Techniques on the Recovery and Quality of Bovine Cumulus Oocyte Complexes
محورهای موضوعی : Camelاس.اچ. بهلولی 1 , اس. بزگلو 2 , اف. سدن 3
1 - Department of Animal Science, Faculty of Agriculture, University of Ankara, Ankara, Turkey
2 - Department of Animal Science, Faculty of Agriculture, University of Ankara, Ankara, Turkey
3 - Department of Animal Science, Faculty of Agriculture, University of Ankara, Ankara, Turkey
کلید واژه: cattle, aspiration, cumulus oocyte complex, oocyte, slicing,
چکیده مقاله :
This study investigated the effect of different harvesting techniques on the recovery rate and quality of bovine cumulus oocyte complexes (COCs) and their subsequent developmental competence including in vitro maturation, fertilization and blastocyst formation. The COCs were retrieved from slaughter ovaries using aspiration, slicing, or aspiration followed by slicing. The COCs were examined microscopically then classed as A (at least three layers of cumulus), B (two layers of cumulus), C (one layer of cumulus, or degraded cytoplasm) or D (abnormal). The retrieved COCs yields per ovary differed (P≤0.05) between aspiration (2.31±0.21), slicing (6.65±1.65) and aspiration followed by slicing (3.72±1.43). Differences in quality of collected COCs were observed for categories A, B and C but not for category D. Oocyte diameter and in vitro developmental competence did not differ between the retrieving methods. In conclusion, aspiration can recover moderate numbers of oocytes ovary and about half of them can not be retrieved by this method. Therefore when oocyte number is important, aspiration should be avoided and ovaries should be sliced.
در این مطالعه اثر تکنیکهای مختلف برداشت بر بازیابی و کیفیت ساختارهای اووسیت کومولوس (COC) گاوی و ویژگیهای تکاملی بعدی آنها شامل بلوغ آزمایشگاهی، باروری و تشکیل بلاستوسیست مورد بررسی قرار گرفته است. COCها از تخمدانهای دامهای ذبح شده با استفاده از مکش، برش یا مکش پس از برش برداشت شدند. COCها به صورت میکروسکوپی کنترل شده و سپس در گروههای A (حداقل سه لایه کومولوس)، B (دو لایه کومولوس)، C (یک لایه کومولوس یا سیتوپلاسم تخریب شده) و D (غیرطبیعی) دستهبندی گردیدند. انواع COCها به ازای هر تخمدان در بین سه روش مکش (21/0±31/2)، برش (65/1±65/6) و مکش پس از برش (43/1±72/3) متفاوت بودند (05/0≥P). تفاوت در کیفیت COCها بین دستههای A، B و C مشاهده گردیده ولی برای دسته D تفاوتی مشاهده نشد. قطر اووسیت و ویژگیهای تکاملی آزمایشگاهی بین روشهای مختلف برداشت تفاوتی نداشتند. نتیجهگیری میشود که مکش میتواند تعداد متوسطی از اووسیتهای تخمدانی را برداشت نموده و حدود نیمی از آنها نمیتوانند توسط این روش برداشت گردند. بنابراین در مواردی که تعداد اووسیتها اهمیت دارد، باید از مکش خودداری کرده و از برش استفاده نمود.
Alm H., Torner H., Kanitz W. and Roschlau K. (2008). Influence of oocyte recovery method, in vitro fertilization method and serum source on embryonic development of in vitro matured bovine oocytes. Arch. Tierz. 51, 224-234.
Alm H., Torner H., Kanitz W., Becker F. and Hinrichs K. (1997). Comparison of different methods for recovery of horse oocytes. Eq. Vet. J. Suppl. 25, 47-50.
Carolan C., Monaghan P., Gallagher M. and Gordon I. (1994). Effect of recovery method on yield of bovine oocytes per ovary and their developmental competence after maturation, fertilization and culture in vitro. Theriogenology. 41, 1061-1068.
Das G.K., Jain G.C., Solanki V.S. and Tripathi V.N. (1996). Efficacy of various collection methods for oocyte retrival in buffalo. Theriogenology. 46, 1403-1411.
Das S.K. and Santra A. (2008). Comparative the efficiency of different oocyte recovery methods from cattle ovaries. Indian J. Anim. Sci. 78, 277-278.
de Mendiburu F. (2014). Agricolae: Statistical Procedures for Agricultural Research. Lima, Peru: National Engineering University.
EL-Harairy M.A., Shamiah S.M., Abdel-Khalek A.E. and Khalil W.A. (2007). Effect of pregnancy status and harvesting technique on ecovery rate and categories of Dromedary camel oocytes. Egypt J. Basic Appl. Physiol. 2, 255-268.
Farahavar A., Shahne A., Kohram H. and Vahedi V. (2010). Effect of melatonin on in vitro maturation of bovine oocytes. African J. Biotechnol. 9, 2579-2583.
Gardner D.K., Michelle L. and Andrew J.W. (2004). A laboratory Guide to The Mammalian Embriyo. New York: Oxford University press.
Hasler J.F. (1998). The current status of oocyte recovery, in vitro embriyo production and embriyo transfer in domestic animals, with an emphasis on the bovine. J. Anim. Sci. 76, 52-74.
Hoque S.A.M., Kabiraj S.K., Khandoker M.A.M.Y., Mondal A. and Tareq K.M.A. (2011). Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes. African J. Biotechnol. 10, 9177-9181.
Mantovani R., Rota A., Penzo N. and Bittante G. (1999). Comparison among oocytes recovery methods for bovine embryo production in vitro. Pp. 235-235 in Proc. 13th A.S.P.A. Cong., Piacenza.
Nowshari M.A. (2005). The effect of harvesting technique on efficiency of oocyte collection and different maturation media on the nuclear maturation of oocytes in camels (Camelus dromedarius). Theriogenology. 63, 2471-2481.
Pawshe C.H., Totey S.M. and Jain S.K. (1994). A comparison of three methods of recovery of goat oocytes for in vitro maturation and feritilization. Theriogenology. 42, 117-125.
Pontes J.H.F., Silva K.C.F., Basso A.C., Rigo A.G., Ferreira C.R., Santos G.M.G., Sanches B.V., Porcionato J.P.F., Vieira P.H.S., Faifer F.S., Sterza F.A.M., Schenk J.L. and Seneda M.M. (2010). Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and Indicus-taurus dairy cows using sexed sperm. Theriogenology. 74, 1349-1355.
Seefeld K. and Linder E. (2007). Statistics Using R with Biological Examples. Durham: University of New Hampshire.
Shabankareh H.K., Sarsaifi K. and Mehrannia T. (2011). In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum. J. Assist. Reprod. Genet. 28, 531-537.
Takagi Y., Mori K., Takahashi T., Sugawara S. and Masaki J. (1992). Differences in development of bovine oocytes recovered by aspiration or by mincing. J. Anim. Sci. 70, 1923-1927.
Tetzner T.A.D., Saraiva N.Z.S., Perecin F., Niciura S.C.M., Ferreira C.R., Oliveira C.S. and Garcia J.M. (2011). The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. R. Brasilian Zootech. 40, 2135-2141.
Tripp M.W., Ju J.C., Hoagland T.A., Riesen J.W., Yang X. and Zinn S.A. (2000). Influence of somatotropin and nutrition on bovine oocyte retrieval and in vitro development. Theriogenology. 53, 1581-1590.
Wang Z.G., Xu Z.R. and Yu S.D. (2007). Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat. Czech J. Anim. Sci. 52, 21-25.
Wani N.A., Wani G.M., Khan M.Z. and Salahudin S. (2000). Effect of oocyte harvesting techniques on in vitro maturation and in vitro fertilization in sheep. Small Rumin. Res. 36, 63-67.
