Effect of Different Harvesting Techniques on the Recovery and Quality of Bovine Cumulus Oocyte Complexes
محورهای موضوعی : Camel
اس.اچ. بهلولی
1
(Department of Animal Science, Faculty of Agriculture, University of Ankara, Ankara, Turkey)
اس. بزگلو
2
(Department of Animal Science, Faculty of Agriculture, University of Ankara, Ankara, Turkey)
اف. سدن
3
(Department of Animal Science, Faculty of Agriculture, University of Ankara, Ankara, Turkey)
کلید واژه: cattle, aspiration, cumulus oocyte complex, oocyte, slicing,
چکیده مقاله :
This study investigated the effect of different harvesting techniques on the recovery rate and quality of bovine cumulus oocyte complexes (COCs) and their subsequent developmental competence including in vitro maturation, fertilization and blastocyst formation. The COCs were retrieved from slaughter ovaries using aspiration, slicing, or aspiration followed by slicing. The COCs were examined microscopically then classed as A (at least three layers of cumulus), B (two layers of cumulus), C (one layer of cumulus, or degraded cytoplasm) or D (abnormal). The retrieved COCs yields per ovary differed (P≤0.05) between aspiration (2.31±0.21), slicing (6.65±1.65) and aspiration followed by slicing (3.72±1.43). Differences in quality of collected COCs were observed for categories A, B and C but not for category D. Oocyte diameter and in vitro developmental competence did not differ between the retrieving methods. In conclusion, aspiration can recover moderate numbers of oocytes ovary and about half of them can not be retrieved by this method. Therefore when oocyte number is important, aspiration should be avoided and ovaries should be sliced.
در این مطالعه اثر تکنیک­های مختلف برداشت بر بازیابی و کیفیت ساختارهای اووسیت کومولوس (COC) گاوی و ویژگی­های تکاملی بعدی آنها شامل بلوغ آزمایشگاهی، باروری و تشکیل بلاستوسیست مورد بررسی قرار گرفته است. COCها از تخمدان­های دام­های ذبح شده با استفاده از مکش، برش یا مکش پس از برش برداشت شدند. COCها به صورت میکروسکوپی کنترل شده و سپس در گروه­های A (حداقل سه لایه کومولوس)، B (دو لایه کومولوس)، C (یک لایه کومولوس یا سیتوپلاسم تخریب شده) و D (غیرطبیعی) دسته­بندی گردیدند. انواع COCها به­ ازای هر تخمدان در بین سه روش مکش (21/0±31/2)، برش (65/1±65/6) و مکش پس از برش (43/1±72/3) متفاوت بودند (05/0≥P). تفاوت در کیفیت COCها بین دسته­های A، B و C مشاهده گردیده ولی برای دسته D تفاوتی مشاهده نشد. قطر اووسیت و ویژگی­های تکاملی آزمایشگاهی بین روش­های مختلف برداشت تفاوتی نداشتند. نتیجه­گیری می­شود که مکش می­تواند تعداد متوسطی از اووسیت­های تخمدانی را برداشت نموده و حدود نیمی از آنها نمی­توانند توسط این روش برداشت گردند. بنابراین در مواردی که تعداد اووسیت­ها اهمیت دارد، باید از مکش خودداری کرده و از برش استفاده نمود.
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