Immunity Against Avian Influenza Viruspanax Ginseng Polysaccharide (GPS) Can Improve Immunity Against H9N2 Avian Influenza Virus in Chickens
محورهای موضوعی : Camel
اس. کالون
1
(Department of Animal Science, South China Agricultural University, Guangzhou 510642, China)
آ.ی. عبدالهی
2
(Department of Animal Science, Kano University of Science and Technology, Wudil, Kano, Nigeria)
کلید واژه: immunity, antibodies, cytokines, H9N2 avian influenza virus, panax ginseng polysac-charide,
چکیده مقاله :
The humoral immunization potential of panax ginseng polysaccharide (GPS) against H9N2 avian influenza virus (H9N2 AIV) in chickens was investigated. The effects of GPS treatment before and during H9N2 AIV infection were determined in chicken embryo fibroblast (CEF) by MTT (3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide) assays and quantitative RT-PCR analysis of MHC and cytokine expression. The percentages of CD3+, CD4+ and CD8+ T cells in peripheral blood lymphocytes and serum antibody titers were examined in vivo. High expressions of MHCII and the cytokines IL-2, IL-4, and IL-10 were observed in CEF treated with GPS before and during H9N2 infection. These results indicated that the antiviral activity of GPS was enhanced by pretreatment of CEF and that GPS promotes early humoral immune responses in young chickens
ظرفیت ایجاد ایمنی هومورال پلی ساکاارید جین­سنگ (GPS) علیه ویروس آنفلوآنزای طیور H9N2 در جوجه­های گوشتی بررسی شد. اثرات تیمار قبل و در طی آلودگی با ویروس آنفلوانزای طیور H9N2 در فیبروبلاست جنین جوجه­ها (CEF) توسط MTT (3(4 و 5-دی متیل تیازول)-2 و 3- دی فتییل تترالیوم­بروماید) و تجزیه کمی MHC با روش RT-PCR و بیان سیتوکین تعیین شد. درصدهای سلول­های TCD3+، CD4+ و CD8+ در لنفوسیت­های خون احشایی و تیترهای آنتی­بادی سرمی در آزمایشگاه تعیین شدند. بیان بالای MHCII و سیتوکین­های IL-2، IL-4 و IL-10 در تیمارهای CEF و GPS قبل و در طی عفونت با H9N2 مشاهده شد. این نتایج مشخص نمود که فعالیت ضد­ویروسی GPS توسط پیش تیمار CEF افزایش یافت و اینکه GPS پاسخ­های ایمنی هومورال اولیه را در جوجه­های جوان تسریع می‌کند.
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