پلی ساکارید جین‌سنگ (Panax ginseng) می‌تواند در جوجه‌های گوشتی ایمنی علیه ویروس آنفلوانزای طیور H9N2 را بهبود دهد

Subject Areas : Camel

اس. کالون ¹ , آ.ی. عبدالهی ²

1 - Department of Animal Science, South China Agricultural University, Guangzhou 510642, China
2 - Department of Animal Science, Kano University of Science and Technology, Wudil, Kano, Nigeria

Received: 2014-07-27 Accepted : 2015-01-31 Published : 2015-09-01

Keywords: آنتی‌بادی‌ها, سیتوکین‌ها, ویروس آنفلوآنزایطیور H9N2, ایمنییت, پلی ساکارید جین‌سنگ,

Abstract :

ظرفیت ایجاد ایمنی هومورال پلی‌ ساکاارید جین­سنگ (GPS) علیه ویروس آنفلوآنزای طیور H9N2 در جوجه­های گوشتی بررسی شد. اثرات تیمار قبل و در طی آلودگی با ویروس آنفلوانزای طیور H9N2 در فیبروبلاست جنین جوجه­ها (CEF) توسط MTT (3(4 و 5-دی متیل تیازول)-2 و 3- دی فتییل تترالیوم­بروماید) و تجزیه کمی MHC با روش RT-PCR و بیان سیتوکین تعیین شد. درصدهای سلول­های TCD3+، CD4+ و CD8+ در لنفوسیت­های خون احشایی و تیترهای آنتی­بادی سرمی در آزمایشگاه تعیین شدند. بیان بالای MHCII و سیتوکین­های IL-2، IL-4 و IL-10 در تیمارهای CEF و GPS قبل و در طی عفونت با H9N2 مشاهده شد. این نتایج مشخص نمود که فعالیت ضد­ویروسی GPS توسط پیش تیمار CEF افزایش یافت و اینکه GPS پاسخ­های ایمنی هومورال اولیه را در جوجه­های جوان تسریع می‌کند.

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