Allelic and Genotypic Distribution in Single Nucleotide Polymorphism (SNP) G.676A > G of Melanocortin-1 Receptor (MC1R) Gene in Indonesian Goat Breeds
محورهای موضوعی : Camel
د. ماهارانی
1
,
اس. الیسر
2
,
آ.جی.اس. بودیساتریا
3
,
آ. باتوبارا
4
,
د.ن.اچ. هاریونو
5
,
آ.پی.ز.ن.ل. ساری
6
1 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Bulaksumur, Yogyakarta 55281, Indonesia
2 - Indonesian Goat Research Institute Sei Putih, Galang 20585, North Sumatera, Indonesia
3 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Bulaksumur, Yogyakarta 55281, Indonesia
4 - Indonesian Goat Research Institute Sei Putih, Galang 20585, North Sumatera, Indonesia
5 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Bulaksumur, Yogyakarta 55281, Indonesia
6 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Bulaksumur, Yogyakarta 55281, Indonesia
کلید واژه: local goat, allele distribution, <i>MC1R</i> gene,
چکیده مقاله :
The melanocortin-1 receptor (MC1R) gene has been investigated by many studies regarding the pigmentation variation in various species. In order to determine its allelic and genotypic distribution, we sequenced the goat MC1R gene from 78 individuals in ten populations (Gembrong, Senduro, Ettawa Grade, Boerawa, Boerka, Kosta, Samosir, Muara, Boer and Kacang). Direct sequencing method was performed to detect the single nucleotide polymorphisms (SNPs). Three SNPs (g.676A>G, g.748G>T and g.801C>G) were identified in the gene target. The SNP g.676 A > G was used to genotype the investigated animals by using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method with EarI restriction enzyme. In all studied populations, the A allele had the highest frequency (83%) and the G allele had the lowest frequency (17%). The frequency of homozygous AA genotype was the highest (90%) in Kacang goats, while that of heterozygous AG genotype was fixed in Senduro and Boer goats. Analysis of observed heterozygosity, expected heterozygosity, Nei’s (1973) expected heterozygosity, and Shannon index were found to be 0.3425, 0.2858, 0.2838 and 0.4578, respectively.The distribution of MC1R gene in overall breeds confirmed with Hardy-Weinberg equilibrium (P>0.05).
ژن گیرنده ملانوکورتین-1 (MC1R) در مطالعات بسیاری در مورد تغییر رنگدانه در گونه‌های مختلف مورد بررسی قرار گرفته است. بر این اساس برای تعیین توزیع آللی و ژنوتیپی آن، ما ژن MC1R بز را از 78 فرد در 10 جمعیت (Gembrong، Senduro، Ettawa Grade، Boerawa، Boerka، Kosta، Samosir، Muara، Boer و Kacang) توالی‌یابی کردیم. روش توالی‌یابی مستقسم برای تشخیص چندشکلی‌های تک نوکلئوتیدی (SNPs) بکار گرفته شد. سه SNPs (g.676A>G، g.748G>T و g.801C>G) در ژن هدف شناسایی شدند. SNP g.676A>G برای ژنوتیپ حیوانات بررسی شده توسط روش PCR-RFLP با آنزیم محدود کننده EarI استفاده شد. در تمامی جمعیت‌های مطالعه شده، آلل A بیشترین فراوانی را داشت (83 درصد) و آلل G کمترین فراوانی را داشت (17 درصد). فراوانی ژنوتیپ هموزیگوس AA در بزهای Kacang بالاترین بود (90 درصد)، اگرچه که ژنوتیپ هتروزیگوس AG در بزهای Senduro و Boer تثبیت شده بود. آنالیز هتروزیگوسیتی مشاهده شده، هتروزیگوسیتی مورد انتظار، هتروزیگوسیتی مورد انتظار Nei’s (1973)، و شاخص شانون به ترتیب 3425/0، 2858/0، 2838/0 و 4578/0 بودند. توزیع ژن MC1R در تمامی نژادها با تعادل هاردی-واینبرگ تأیید شد (05/0<P).
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