The Effect of Uncoupling Protein Polymorphisms on Growth, Breeding Value of Growth and Reproductive Traits in the Fars Indigenous Chicken
محورهای موضوعی : Camelآ. محمدی‌فر 1 , م.ر. محمدآبادی 2
1 - Department of Animal Science, Faculty of Agriculture, Payame Noor University, Kerman, Iran
2 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
کلید واژه: PCR-RFLP, polymorphism, Fars indigenous chicke, reproductive trait, uncoupling pro-tein,
چکیده مقاله :
The avianuncoupling protein (avUCP) is a member of the mitochondrial transporter superfamily that uncouples proton entry in the mitochondrial matrix from ATP synthesis. The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method was used to estimate the allele and genotype frequencies of the UCP/HhaI polymorphisms and to determine associations between these polymorphisms and the growth traits, breeding value of growth and reproductive traits in the Fars indigenous chicken. For this purpose phenotype information of 18 successive generations from 200 birds were analyzed using a univariate animal model in ASREML procedure. The evaluation of the association between this single nucleotide polymorphisms (SNP) with reproductive traits suggests a positive effect of TC genotype with age at first egg (ASM) compared with CC genotype. In addition, TC genotype was significantly associated with the breeding value of age at first egg compared with the CC genotype (P<0.05). In conclusion, our results suggest that the TC genotype of the UCP gene is associated with age at sexual maturity (ASM) and breeding value of age at sexual maturity and UCP polymorphisms may be used as DNA markers for selection in the breeding process of the Fars indigenous chicken.
پروتئین غیر مزدوج طیور (avUCP) عضوی از فوق خانواده ناقلین میتوکندریایی است که ورود پروتئین به ماتریکس میتوکندری را از سنتز ATP آزاد میکند. از روش PCR-RFLP برای برآورد فراوانیهای آللی و ژنوتیپی چندشکلیهای UCP/HhaI استفاده شد و همبستگی بین این چندشکلیها و صفات رشد، ارزش اصلاحی صفات رشد و تولیدمثلی در مرغ بومی فارس محاسبه شد. برای این منظور اطلاعات فنوتیپی 18 نسل متوالی از 200 پرنده با استفاده از مدل حیوانی تک صفتی با روش ASREML آنالیز شد. ارزیابی همبستگی بین این SNP با صفات تولیدمثلی اثر مثبت ژنوتیپ TC را با سن اولین تخمگذاری (ASM) در مقایسه با ژنوتیپ CC پیشنهاد میکند. به علاوه، ژنوتیپ TC همبستگی معنی‌داری با ارزش اصلاحی سن اولین تخمگذاری در مقایسه با ژنوتیپ CC نشان داد (05/0>P). در نتیجه، نتایج این پژوهش پیشنهاد میکند که ژنوتیپ TC ژن UCP با سن بلوغ جنسی (ASM) و ارزش اصلاحی سن بلوغ جنسی همبستگی دارد و چندشکلیهای UCP میتوانند به عنوان نشانگرهای DNA برای انتخاب در فرآیند اصلاح نژاد مرغ بومی فارس مورد استفاده قرار گیرند.
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