Effect of Single Nucleotide Polymorphisms in IGF-1R Gene on Growth Rate Traits in Makooei Sheep
محورهای موضوعی : Camelم. پسندیده 1 , ق. رحیمی 2 , و. همتی 3
1 - Department of Genetics and Animal Breeding, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Genetics and Animal Breeding, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Genetics and Animal Breeding, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
کلید واژه: Makooei sheep, PCR-SSCP, growth rate traits, <i>IGF-1R</i>,
چکیده مقاله :
Insulin-like growth factor 1 receptor (IGF-1R) is a main receptor of IGFs family which plays a critical role in the postnatal growth and skeletal growth in many species. However, there are few reports of IGF-1R gene structure and its effects on growth traits in sheep. The objectives of this study were detection of IGF-1R polymorphisms and assessment of their associations with growth traits in Iranian Makooei Sheep. Hence, 200 Makooei lambs were genotyped through polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP). The studied traits were birth weight (BW), weaning weight (WW), 6 months weight (6MW), average daily gains from birth to 3 months (ADG0-3), from 3 months to 6 months (ADG3-6), from birth to 6 months (ADG0-6) and corresponding Kleiber ratios (KR0-3, KR3-6, KR0-6).For this genetic position, three types of banding patterns (AA, AB and BB) were identified with the frequencies of 0.69, 0.16 and 0.15, respectively. In this study, IGF-1R genotypes indicated the significant associations with 6MW, ADG0-6, KR0-3 (P<0.05) and ADG0-3 (P<0.01). In all of the significant traits, The AAgenotype was linked to the highest values, while the BBgenotype was linked to the lowest values. The results of this study indicated that single nucleotide polymorphism (SNP) variation in IGF-1R gene can be used as a molecular marker for improving of growth traits in marker assisted selection programs in sheep.
گیرنده فاکتور رشد شبه انسولین نوع I (IGF-1R) یک گیرنده اصلی از خانواده IGFs می‌باشد که نقش اساسی در رشد پس از تولد و نیز رشد ماهیچه اسکلتی در بسیاری از گونه‌ها ایفا می‌نماید. با این حال، تعداد کمی مطالعه درباره ساختار و اثر ژن IGF-1R روی صفات رشد در گوسفند وجود دارد به طوریکه تاکنون هیچ تحقیقی در مورد ارتباط این ژن با صفات رشد در نژادهای گوسفند ایرانی انجام نشده است. اهداف این تحقیق، شناسایی چندشکلی‌های ژن IGF-1R و بررسی ارتباط آنها با صفات رشد در گوسفند نژاد ماکویی بودند. از این رو، 200 رأس بره ماکویی با واکنش زنجیره‌ای پلی‌مراز چندشکلی فضایی تک رشته‌ای (PCR-SSCP) تعیین ژنوتیپ شدند. صفات مورد مطالعه شامل وزن تولد (BW)، وزن شیرگیری (WW)، وزن شش ماهگی (6MW)، میانگین رشد روزانه از تولد تا سه ماهگی (ADG0-3)، از سه ماهگی تا شش ماهگی (ADG3-6)، از تولد تا شش ماهگی (ADG0-6) و نسبت‌های کلیبر متناظر با آنها (KR0-6, KR3-6, KR0-3) بودند.برای این موقعیت ژنتیکی، سه الگوی باندی AA، AB و BB با فراوانی‌های به ترتیب 69/0، 16/0 و 15/0 شناسایی شدند. در این مطالعه، ژنوتیپ‌های مختلف IGF-1R با صفات 6MW، ADG(0-6)، KR(0-3) (05/0>P) و ADG(0-3) (01/0>P) ارتباط معنی‌دار نشان دادند. در همه صفات معنی‌دار، ژنوتیپ AA با بالاترین مقادیر و ژنوتیپ BB با کمترین مقادیر مرتبط بودند. نتایج این تحقیق نشان داد که تنوع در ژن IGF-1R می‌تواند به عنوان یک نشانگر مولکولی برای بهبود صفات رشد در برنامه‌های انتخاب به کمک نشانگر در گوسفند استفاده شود.
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