اثر چندشکلی‏های تک نوکلئوتیدی ژن IGF-1R روی صفات نرخ رشد در گوسفند نژاد ماکویی

Subject Areas : Camel

م. پسندیده ¹ , ق. رحیمی ² , و. همتی ³

1 - Department of Genetics and Animal Breeding, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Genetics and Animal Breeding, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Genetics and Animal Breeding, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran

Received: 2018-11-16 Accepted : 2019-01-30 Published : 2019-12-01

Keywords: PCR-SSCP, گوسفند ماکویی, صفات نرخ رشد, IGF-1R,

Abstract :

گیرنده فاکتور رشد شبه انسولین نوع I (IGF-1R) یک گیرنده اصلی از خانواده IGFs می‌باشد که نقش اساسی در رشد پس از تولد و نیز رشد ماهیچه اسکلتی در بسیاری از گونه‌ها ایفا می‌نماید. با این حال، تعداد کمی مطالعه درباره ساختار و اثر ژن IGF-1R روی صفات رشد در گوسفند وجود دارد به طوریکه تاکنون هیچ تحقیقی در مورد ارتباط این ژن با صفات رشد در نژادهای گوسفند ایرانی انجام نشده است. اهداف این تحقیق، شناسایی چندشکلی‌های ژن IGF-1R و بررسی ارتباط آنها با صفات رشد در گوسفند نژاد ماکویی بودند. از این رو، 200 رأس بره ماکویی با واکنش زنجیره‌ای پلی‌مراز چندشکلی فضایی تک رشته‌ای (PCR-SSCP) تعیین ژنوتیپ شدند. صفات مورد مطالعه شامل وزن تولد (BW)، وزن شیرگیری (WW)، وزن شش ماهگی (6MW)، میانگین رشد روزانه از تولد تا سه ماهگی (ADG0-3)، از سه ماهگی تا شش ماهگی (ADG3-6)، از تولد تا شش ماهگی (ADG0-6) و نسبت‌های کلیبر متناظر با آ‌نها (KR0-6, KR3-6, KR0-3) بودند.برای این موقعیت ژنتیکی، سه الگوی باندی AA، AB و BB با فراوانی‌های به ترتیب 69/0، 16/0 و 15/0 شناسایی شدند. در این مطالعه، ژنوتیپ‌های مختلف IGF-1R با صفات 6MW، ADG(0-6)، KR(0-3) (05/0>P) و ADG(0-3) (01/0>P) ارتباط معنی‌دار نشان دادند. در همه صفات معنی‌دار، ژنوتیپ AA با بالاترین مقادیر و ژنوتیپ BB با کمترین مقادیر مرتبط بودند. نتایج این تحقیق نشان داد که تنوع در ژن IGF-1R می‌تواند به عنوان یک نشانگر مولکولی برای بهبود صفات رشد در برنامه‌های انتخاب به کمک نشانگر در گوسفند استفاده شود.

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