Effect of in ovo Injection of Calcium Carbonate Nanoparticles on Bone Post Hatched Characteristics and Broiler Chicken Performance
محورهای موضوعی : Camelج. سالاری 1 , ح.ر. همتی متین 2 , ک. غفاری 3 , ه. حاجتی 4
1 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
2 - Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
3 - Department of Animal Veterinary, Ferdowsi University of Mashhad, Mashhad, Iran
4 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
کلید واژه: broiler performance, calcium carbonate nanoparticles, <i>in ovo</i>,
چکیده مقاله :
This experiment was conducted to evaluate the effect of in ovo injection (IOI) of calcium carbonate nano-particles (CCN) on post-hatch bone and blood parameters and performance of broiler chickens. Fertile eggs (n=400) were distributed into 4 groups of 100 eggs. On the first day of incubation, 2 groups were injected with 0.5 mL of either 100 or 200 ppm CCN/egg dissolved in normal saline and two groups were used as sham control (injected with 0.5 mL normal saline) or un-injected control. The hatched chicks from each group were randomly assigned to 4 replications of 15 chickens and reared under standard condition. The results demonstrated that body weight, spleen weight, and bone Ca concentration of hatched chickens was higher by IOI of 200 ppm/egg CCN (P<0.05). The highest bursa weight was obtained by IOI of 200 ppm/egg CCN (P<0.05). The IOI of CCN at levels of 100 or 200 ppm/egg was significantly increased plasma alkaline phosphatase activity and bone Cu concentration of hatched chickens (P<0.05). Moreover, IOI had no significant effects on performance at 1 to 21 days of age (P<0.05). These preliminary results suggest that growth and development of bone may accelerate through IOI of CCN at levels of 100 or 200 ppm/egg on 1-d of incubation.
این آزمایش برای ارزیابی تأثیر تزریق داخل تخم مرغی نانوذرات کربنات کلسیم بر خصوصیات استخوان، فراسنجههای خونی و عملکرد جوجههای گوشتی بعد از تفریخ انجام شد. تخم مرغهای بارور (400 عدد) به 4 گروه هر کدام شامل 100 عدد تخم مرغ اختصاص داده شد. در روز اول انکوباسیون، نانوذرات کربنات کلسیم در سطوح 100، 200 میلیگرم/لیتر در 5/0 سیسی سرم فیزیولوژی به دو گروه تزریق شد، یک گروه فقط 5/0 سیسی سرم فیزیولوژی دریافت کرد و گروه دیگر مورد تزریق قرار نگرفت. جوجههای متولد شده از هر گروه به طور تصادفی به 4 گروه هر کدام شامل 15 جوجه اختصاص داده شد و تحت شرایط طبیعی پرورش داده شدند. نتایج نشان داد که وزن بدن، وزن طحال و کلسیم استخوان جوجههای متولد شده در گروهی که سطح 200 میلیگرم/لیتر نانوذرات کربنات کلسیم دریافت کرده بودند بیشتر شد (05/0P<). بیشترین وزن بورس در گروهی که سطح 200 میلیگرم/لیتر نانوذرات کربنات کلسیم دریافت کرده بودند مشاهده شد (05/0P<). تزریق سطوح 100 یا 200 میلیگرم/لیتر نانوذرات کربنات کلسیم به طور معنیداری سبب افزایش فعالیت آلکالین فسفاتاز پلاسما و غلظت مس استخوان جوجههای متولد شده گردید (05/0P<). تزریق داخل تخم مرغی تأثیر معنیداری بر عملکرد جوجهها در سنین 1 تا 21 روزگی نداشت (05/0P<). این نتایج اولیه نشان میدهد که تزریق 100 یا 200 میلیگرم/لیتر نانوذرات کربنات کلسیم در روز اول انکوباسیون میتواند سبب افزایش سرعت رشد و توسعه استخوان شود.
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