Growth Performance, Rumen and Cecum Fermentation Parameters, and Microbial Protein Synthesis in Kermani Lambs with Divergent Residual Feed Intake Fed Forage and Concentrate Diets
محورهای موضوعی : Camel
M. Gevari
1
(Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran)
M.R. Dehghani
2
(Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran)
M. Yousef Elahi
3
(Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran)
R. Hoshyar
4
(Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA|Department of Ruminant and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
کلید واژه: Sheep, feed efficiency, Fermentation, Cecum, residual feed intake,
چکیده مقاله :
< p style="text-align: justify;">It was hypothesized that some of the variations in rumen and cecum fermentation parameters can form a share of individual differences resulting in feed efficiency, which may be altered based on the type of diet. This research aimed to determine the effects of dietary effect and feed efficiency of growing lambs (Kermani lambs) on their rumen and cecum indices and also microbial protein synthesis in gastrointestinal tract. Lambs (n=40; bodyweight (BW)=16±1.5 kg) were fed either a concentrate (CONC; 11.8% crude protein (CP), 18% neutral detergent fiber (NDF), 2.66 Mcal/kg metabolizable energy (ME); n=20) or a forage-based diet (FOR; 15.6% CP, 36.8% NDF, 2.15 Mcal/kg ME; n=20). Individual intake was recorded and residual feed intake (RFI) was determined over 42 days. The 8 highest (Low-RFI) and 8 lowest efficiencies (High RFI) records of lambs from each dietary group were selected (n=16; average BW=20±2.1 kg), and the samples of rumen and cecum fluid, and also urine were collected at the end of the trial. Data were analyzed as a 2 × 2 factorial design with RFI class (high vs. low efficiency), their type of diet (FOR vs. CONC), and their interaction in the defined model. Based on the results, high-efficiency lambs had a higher level (p < 0.01) of total volatile fatty acid (VFA), proportional concentrations of acetate, propionate, and ammonia nitrogen in the rumen in comparison to low-efficiency lambs. Higher (p < 0.01) amounts of allantoin, xanthine + hypoxanthine, total purine derivative (PD), microbial nitrogen and microbial protein were observed in the high efficiency than low-efficiency lambs. The low efficiency lambs had greater (p < 0.01) proportional acetate, cecal pH and cecal ammonia N compared to high-efficiency lambs. The RFI class × diet type interaction was significant (p < 0.01) for the majority of parameters of the rumen, cecum, and microbial protein synthesis. The results of this experiment exhibited that hindgut fermentation especially cecum played a key role in the efficiency of feed utilization in lambs which have consumed larger amounts of fermentable substrates.
Ampapon T., Phesatcha K. and Wanapat M. (2019). Effects of phytonutrients on ruminal fermentation, digestibility, and microorganisms in Swamp Bualoes. Animal. 9, 1-9.
AOAC. (2005). Official Methods of Analysis. 18th Ed. Association of Official Analytical Chemists, Arlington, Washington, DC., USA.
Bach A., Calsamiglia S. and Stern M.D. (2005). Nitrogen metabolism in the rumen. J. Dairy Sci. 88(1), 9-21.
Borton R.J., Loerch S.C., McClure K.E. and Wulf D.M. (2005). Comparison of characteristics of lambs fed concentrate or grazed on ryegrass to traditional or heavy slaughter weights. I. Production, carcass, and organoleptic characteristics. J. Anim. Sci. 83, 679-685.
Carberry C.A., Kenny D.A., Han S., McCabe M.S. and Waters S.M. (2012). Effect of phenotypic residual feed intake and dietary forage content on the rumen microbial community of beef cattle. Appl. Environ. Microbiol. 78(14), 4949-4958.
Cardozo P., Calsamiglia S. and Ferret A. (2000). Effect of pH on microbial fermentation and nutrient flow in a dual flow continuous culture system. J. Dairy Sci. 83(1), 265-272.
Cardozo P., Calsamiglia S. and Ferret A. (2002). Effects of pH on nutrient digestion and microbial fermentation in a dual flow continuous culture system fed a high concentrate diet. J. Dairy Sci. 85(1), 182-190.
Chen X.B. and Gomes M.J. (1992). Estimation of Microbial Protein Supply to Sheep and Cattle based on Urinary Excretion of Purine Derivatives-an Overview of Technical Detail. Occasional Publication of the International Feed Resources Units, Rowett Research Institute, Aberdeen, United Kingdom.
Cockrum R.R., Stobart R.H., Lake S.L. and Cammack K.M. (2013). Phenotypic variation in residual feed intake and performance traits in rams. Small Rumin. Res. 113, 313-322.
De Gregorio R.M., Tucker R.E., Mitchell G.E.Jr. and Gill W.W. (1984). Acetate and propionate production in the cecum and proximal colon of lambs. J. Anim. Sci. 58, 203-207.
Dijkstra J., Ellis L., Kebreab E., Strathe A.B., Lopez S., France J. and Bannink A. (2012). Ruminal pH regulation and nutritional consequences of low pH. Anim. Feed Sci. Technol. 172, 22-33.
Dixon R.M. and Nolan J.V. (1986). Nitrogen and carbon flows between the caecum, blood and rumen in sheep given chopped lucerne (Medicago sativa) hay. Br. J. Nutr. 55, 313-332.
Elliott R. and Little D.A. (1977). Fate of cyst(e)ine synthesized by microbial activity in the ruminant caecum. Australian J. Biol. Sci. 30, 203-206.
Ellison M.J., Conant G.C., Lamberson W.R., Cockrum R.R., Austin K.J., Rule D.C. and Cammack K.M. (2017). Diet and feed efficiency status affect rumen microbial profiles of sheep. Small Rum. Res. 156, 12-19.
Eugene M., Archimede H. and Sauvant D. (2004). Quantitative meta-analysis on the effects of defaunation of the rumen on growth, intake and digestion in ruminants. Livest. Prod. Sci. 85, 81-97.
Ferrell C.L. and Jenkins T.G. (1998). Body composition and energy utilization by steers of diverse genotypes fed a high-concentrate diet during the finishing period: II. Angus, Boran, Brahman, Hereford, and Tuli steers. J. Anim. Sci. 76, 647-657.
Fujihara T., Shem M.N. and Matsui T. (2007). Urinary excretion of purine derivatives and plasma allantoin level in sheep and goats during fasting. J. Anim. Sci. 78, 129-134.
Gressley T.F., Hall M.B. and Armentano L.E. (2011). Ruminant nutrition symposium: Productivity, digestion, and health responses to hindgut acidosis in ruminants. J. Anim. Sci. 89, 1120-1130.
Guan L.L., Nkrumah J.D., Basarab J.A. and Moore S.S. (2008). Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle’s feed efficiency. FEMS Microbiol. Lett. 288, 85-91.
Hatfield P.G., Hopkins J.A., Pritchard G.T. and Hunt C.W. (1997). The effects of amounts of whole barley, barley bulk density, and form of roughage on feedlot lamb performance, carcass characteristics, and digesta kinetics. J. Anim. Sci. 75, 3353-3366.
Horadagoda A., Fulkerson W.J., Barchia I., Dobos R.C. and Nandra K.S. (2008). The effect of grain species, processing and time of feeding on the efficiency of feed utilization and microbial protein synthesis in sheep. Livest. Sci. 114, 117-126.
Hungate R.E. (1966). Quantities of carbohydrate fermentation products. Pp. 245-272 in the Rumen and Its Microbes. Academic Press, New York.
Kiyoshi T., Shozo A., Koretsugu O., Takafumi N., Hiroki M., Mutsumi N., Rustam I.A. and Yoshimi B. (2000). Rumen bacterial community transition during adaptation to high-grain diet. Anaerobe. 6, 273-284.
Koch R.M., Swiger L.A., Chambers D. and Gregory K.E. (1963). Efficiency of feed use in beef cattle. J. Anim. Sci. 22, 486-494.
Lawrence P., Kenny D.A., Earley B., Crews J.D.H. and McGee M. (2011). Grass silage intake, rumen and blood variables, ultrasonic and body measurements, feeding behavior, and activity in pregnant beef heifers differing in phenotypic residual feed intake. J. Anim. Sci. 89, 3248-3261.
Lewis S.M. and Dehority B.A. (1985). Microbiology and ration digestibility in the hindgut of the ovine. Appl. Environ. Microbiol. 50, 356-363.
Liang Y.S., Li G.Z., Li X.Y., Lü J.Y., Li F.D., Tang D.F., Li F., Deng Y., Zhang H., Wang Z.L. and Weng X.X.(2017). Growth performance, rumen fermentation, bacteria composition, and gene expressions involved in intracellular pH regulation of rumen epithelium in finishing Hu lambs differing in residual feed intake phenotype. J. Anim. Sci. 95, 1727-1738.
Li W., Gelsinger S., Edwards A., Riehle C. and Koch D. (2019). Transcriptome analysis of rumen epithelium and meta-transcriptome analysis of rumen epimural microbial community in young calves with feed induced acidosis. Sci. Rep. 9, 1-13.
Mann S.O. and Orskov E.R. (1973). The rffect of rumen and post-rumen feeding of carbohydrates on the caecal microflora of Sheep. J. Appl. Bacteriol. 36, 475-484.
Myers L.L., Jackson H.D. and Packett L.V. (1967). Absorption of volatile fatty acids form the cecum of sheep. J. Anim. Sci. 26, 1450-1458.
Orskov E.R., Fraser C., Mason V.C. and Mann S.O. (1970). Influence of starch digestion in the large intestine of sheep on caecal fermentation, caccal microflora and faecal nitrogen excretion. British J. Nutr. 24, 671-682.
Pierce K.M., Sweeney T., Brophy P.O., Callan J.J., Fitzpatrick E., Mccarthy P. and O’Doherty J.V. (2006). The effect of lactose and inulin on intestinal morphology, selected microbial populations and volatile fatty acid concentrations in the gastro-intestinal tract of the weanling pig. J. Anim. Sci. 82, 313-318.
Rius A.G., Kittelmann S., Macdonald K.A., Waghorn G.C., Janssen P.H. and Sikkema E. (2012).Nitrogen metabolism and rumen microbial enumeration in lactating cows with divergent residual feed intake fed high-digestibility pasture. J. Dairy Sci. 95(9), 5024-5034.
SAS Institute. (2004). SAS®/STAT Software, Release 9.2. SAS Institute, Inc., Cary, NC. USA.
Sharma V.C., Kundu S.S., Prustyc S., Dattb C. and Kumar M. (2016). Nutrient utilisation, growth performance and blood metabolites in Murrah buffalo calves (Bubalus bubalis) divergently selected for residual feed intake. Arch. Anim. Nutr. 70, 455-469.
Siciliano-jones J. and Murphy M.R. (1989). Production of volatile fatty acids in the rumen and cecum-colon of steers as affected by forage:concentrate and forage physical. J. Dairy Sci. 72(2), 485-492.
Smith S.N., Davis M.E. and Loerch S.C. (2010). Residual feed intake of Angus beef cattle divergently selected for feed conversion ratio. Livest. Sci. 132, 41-47.
Steyn Y., Van Marle-Köster E. and Theron H.E. (2014). Residual feed intake as selection tool in South African Bonsmara cattle. Livest. Sci. 164, 35-38.
Van Soest P.J. (1994). Nutritional Ecology of the Ruminant. Cornell University Press, Ithaca, New York.
Van Soest P.J., Mascarenhas-Ferriera A. and Hartley R.D. (1991). Chemical properties of fiber in relation to nutritive quality of ammonia treated forages. Anim. Feed. Sci. Technol. 10, 155-159.
Morris D.L., Tebbe A.W., Weiss W.P. and Lee C. (2019). Estimating digestible energy values of feeds and diets and integrating those values into net energy systems. J. Dairy Sci. 102, 5212-5218.
Vitorino F.G., Goldfarb K.C., Karaoz U., Leal S., Amado M.A.G., Hugenholtz P., Tringe S.G., Brodie E.L. and Bello M.G.D. (2012). Comparative analyses of foregut and hindgut bacterial communities in hoatzins and cows. ISME J. 6(3), 531-541.
Zhang X., Wang W., Mo F., La Y., Li C. and Li F. (2017). Association of residual feed intake with growth and slaughtering performance, blood metabolism, and body composition in growing lambs. Sci. Rep. 7, 1-11.
Zhou M., Hernandez-Sanabria E. and Guan L.L. (2010). Characterization of variation in rumen methanogenic communities under different dietary and host feed efficiency conditions, as determined by PCR-denaturing gradient gelelectrophores is analysis. Appl. Environ. Microbiol. 76, 3776-3786.
