The Effect of Cattle Seminal Plasma Crude Protein on the Cryopreservation of Goat Semen

محورهای موضوعی : Camel

ت. وایو سوپرایوگی ¹ , س. سوسیلواتی ²

1 - Department of Reproduction, Faculty of Veterinary, Airlangga University, Surabaya, East Java, Indonesia
2 - Department of Reproduction, Faculty of Veterinary, Airlangga University, Surabaya, East Java, Indonesia

تاریخ دریافت : 1396/12/03 تاریخ پذیرش : 1397/02/10 تاریخ انتشار : 1397/09/10

کلید واژه: viability, cattle seminal plasma crude protein, cytochrome C, motility,

چکیده مقاله :

This study aimed to improve the quality of cryopreserved goat semen for artificial insemination by the addition of cattle seminal plasma crude protein. Semen collected from cattle and centrifuged to obtain seminal plasma. Then it was purified to obtain the crude protein. The diluent used in this study was milk-yolk. There were three groups, control group without crude protein (P0), the addition of 2.5 mg crude protein/mL diluent (P1); and the addition of 5 mg crude protein/mL diluent (P2). The results showed that P1 had the highest percentage of progressive motility and viability. Meanwhile, P1 had the lowest percentage of cytochrome C expression. The lowest cytochrome C expression indicates that the mitochondria have a good function. So, by the addition of 2.5 mg crude protein/mL diluent, the quality of frozen goat semen in terms of sperm motility, viability, and cytochrome C expression in the spermatozoa could be improved.

چکیده انگلیسی:

این پژوهش با هدف بهبود کیفیت انجماد اسپرم بز برای تلقیح مصنوعی توسط اضافه کردن پروتئین خام پلاسمای سمینال گاوی انجام شد. اسپرم از گاوها جمع‌آوری شد و برای به‌دست آوردن پلاسمای سمینال سانتریفیوژ شد. سپس برای به‌دست آوردن پروتئین خام تخلیص شد. رقیق‌کننده استفاده شده در این پژوهش زرده‌ تخم‌ مرغ بود. سه گروه وجود داشت، گروه شاهد بدون پروتئین خام (P0)؛ اضافه کردن 2.5 میلی‌گرم پروتئین خام به ازای میلی‌لیتر رقیق­کننده (P1)؛ و اضافه کردن 5 میلی‌گرم پروتئین خام به ازای میلی‌لیتر رقیق­کننده (P2). نتایج نشان دادند که P1 بیشترین درصد حرکت پیش‌رونده و زنده‌مانی را داشت. در این بین، P1 کمترین درصد بیان سیتوکروم C را داشت. کمترین درصد بیان سیتوکروم C نشان می‌دهد که میتوکندری عملکرد خوبی دارد. بنابراین، با اضافه کردن 2.6 میلی‌گرم پروتئین خام به ازای میلی‌لیتر رقیق‌کننده، کیفیت اسپرم فریز شده بز به لحاظ جنبایی اسپرم، زنده‌مانی، و بیان سیتوکروم C در اسپرماتوزوآ می‌تواند بهبود یابد.

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