B and T-Cell Epitope Prediction of the OMP25 Antigen for Developing Brucella melitensis Vaccines for Sheep
محورهای موضوعی : Camelس. یوسفی 1 , م. طهمورث‌پور 2 , م.ه. سخاوتی 3
1 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
کلید واژه: brucella, epitope prediction, OMP25,
چکیده مقاله :
Brucellosis, produced by Brucella species, is a disease that causes severe economic losses for livestock farms worldwide Due to serious economic and medical consequences of this disease, many efforts have been made to prevent the infection through the use of recombinant vaccines based on Brucella outer membrane protein (OMP) antigens. In the present study, a wide range of on-line prediction software was used to predict B and T-cells epitopes, secondary and tertiary structure and antigenicity OMP25 antigens. The bioinformatics approach used in the present study was validated by comparing its results with four available experimental epitope predictions. Bioinformatics analysis identified B-cell epitopes locations at amino acid (AA) residues 26-44, 59-79, 88-112, 146-166 and 175-202l and T-cell epitopes at AA residues 1-10, 14-22, 122-132, 154-162 and 206-213. All final B and T-cell predicted epitopes, except 1-10 and 14-22 residuals, showed antigenicity ability. Finally, a common B and T-cell epitope was identified at 154-162 of the OMP25 antigen. Bioinformatics analysis showed that this region has proper epitope characterization and so may be useful for producing recombinant vaccine.
بروسلوز یکی از رایج ترین بیماری‌های دامی است که توسط باکتری گرم منفی بروسلا ایجاد میشود. با توجه به ضررهای جدی اقتصادی و درمانی این بیماری که برای دام و انسان همواره به ارمغان دارد تلاشهای بسیاری جهت جلوگیری و درمان این بیماری توسط واکسنهای نوترکیب بر پایه آنتیژنهای غشای پروتئینی خارجی صورت میگیرد. بدین منظور هدف از مطالعه حاضر بررسی خصوصیات بیوانفورماتیکی آنتی ژن Omp25 به عنوان یکی از آنتیژنهای غالب غشای پروتئینی باکتری بروسلا بوده است. در این پژوهش از نرم افزارهای بیوانفورماتیکی مختلفی برای پیش‌بینی اپی توپهای B وT، ساختار دوم و سوم پروتئین، خصوصیات ایمنی‌زایی و ویژگیهای هضم پروتئین استفاده گردید. پیش از استفاده از نرم افزار‌ها میزان دقت آنها توسط داده‌های تجربی اعتبار سنجی گردید. نتایج آنالیز بیوانفورماتیکی نشان داد که پنج اپی توپ برای سلول‌های B در موقعیت‌های 44-26، 79-59، 112-88، 166-146، 202-175 و پنج اپی توپ برای سلول‌های T در مکان‌های 10-1، 22-14، 132-122، 162-154 و 213-206 وجود دارد. تمامی اپی توپهای شناسایی شده به جز اپی توپهای 10-1 و 22-14 دارای توانایی ایمنی‌زایی بودند. نهایتاً اپی توپ ناحیه 162-154 به عنوان یک اپی توپ مشترک بین سلول‌های B و T جهت طراحی واکسن نوترکیب پیش‌بینی گردید.
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